1887

Journal of General Virology header logo

Volume 69, Issue 8

Comparison between Parainfluenza Virus Type 2 and Simian Virus 5: Monoclonal Antibodies Reveal Major Antigenic Differences Free

Abstract

Summary

Marked differences in the apparent s of the HN, NP and F proteins of simian virus 5 (SV5) and parainfluenza virus type 2 (PF-2) were revealed by SDS-PAGE. To examine the antigenic relationships between SV5, PF-2 and other paramyxoviruses, monoclonal antibodies (MAbs) specific to PF-2 were isolated. These antibodies had specificities for the HN, NP and P proteins and together with 54 MAbs to SV5 were tested for their ability to react with SV5, PF-2, PF-3, mumps and measles virus proteins. Most of these MAbs (55 out of 60) reacted with homologous virus only. However, five reacted with both SV5 and PF-2. These antibodies had reactivities to the NP, M and P proteins. Furthermore, one of the antibodies with reactivity to the P protein also reacted with mumps virus. Although none of the 21 MAbs with specificities for the HN protein of either SV5 or PF-2 cross-reacted with heterologous virus, some antigenic similarities between the HN protein of SV5 and PF-2 could be detected. This was demonstrated by raising a series of polyclonal antisera to purified preparations of SV5 or PF-2 HN proteins in BALB/c mice, and testing for their ability to neutralize both SV5 and PF-2 and also to immunoprecipitate the HN proteins of these viruses. Surprisingly, while low levels of cross-neutralizing antibody could be detected in some sera (e.g. neutralization of SV5 1:1600 and of PF-2 1:80), other sera with similar neutralization titres against homologous virus failed to neutralize heterologous virus. Furthermore, only a minority of the anti-HN antisera showed any immune-precipitating activity against the heterologous HN protein.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antigenic differences , paramyxoviruses , PF-2 and SV5
© Society for General Microbiology 1988
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-8-2051
1988-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/8/JV0690082051.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-8-2051&mimeType=html&fmt=ahah

References

  1. COWLEY J. A., BARRY R. D. 1983; Characterization of human parainfluenza viruses. 1. The structural proteins of parainfluenza virus 2 and their synthesis in infected cells. Journal of General Virology 64:2117–2125
     [Google Scholar]
  2. GOSWAMI K. K. A., RUSSELL W. C. 1982; A comparison of paramyxoviruses by immunoprecipitation. Journal of General Virology 60:177–183
     [Google Scholar]
  3. GOSWAMI K. K. A., RUSSELL W. C. 1983; Monoclonal antibodies against human paramyxovirus type 3 and against SV5 virus: preparation and preliminary characterization. Journal of General Virology 64:1663–1672
     [Google Scholar]
  4. GOSWAMI K. K. A., LANGE L. S., MITCHELL D. N., CAMERON K. R., RUSSELL W. C. 1984; Does simian virus 5 infect humans?. Journal of General Virology 65:1295–1303
     [Google Scholar]
  5. HERRLER G., COMPANS R. W. 1982; Synthesis of mumps virus polypeptides in infected Vero cells. Virology 100:433–449
     [Google Scholar]
  6. HSIUNG G. D. 1972; Parainfluenza-5 virus. Infection of man and animals. Progress in Medical Virology 14:241–274
     [Google Scholar]
  7. ITO Y., TSURUDOME M., HISHIYAMA M., YAMADA A. 1987; Immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. Journal of General Virology 68:1289–1297
     [Google Scholar]
  8. KINGSBURY D. W., BRATT M. A., CHOPPIN P. W., HANSON R. P., HOSAKA Y., TER MEULEN V., NORRBY E., PLOWRIGHT W., ROTT R., WUNNER W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
     [Google Scholar]
  9. ÖRVELL C, RYDBECK R., LOVE A. 1986; Immunological relationships between mumps virus and parainfluenza viruses studied with monoclonal antibodies. Journal of General Virology 67:1929–1939
     [Google Scholar]
  10. PATERSON R. G., HARRIS T. J. R., LAMB R. A. 1984; Analysis and gene assignment of mRNAs of a paramyxovirus, simian virus 5. Virology 138:310–320
     [Google Scholar]
  11. PRINGLE C. R. 1987 Paramyxoviruses and disease. The Molecular Basis of Virus Disease Society for General Microbiology, Symposium 4051–90 Edited by Russell W. C., Almond J. W. Cambridge: Cambridge University Press;
     [Google Scholar]
  12. RANDALL R. E., DINWOODIE N. 1986; Intranuclear localization of herpes simplex virus immediate-early and delayed-early proteins: evidence that ICP 4 is associated with progeny virus DNA. Journal of General Virology 67:2163–2177
     [Google Scholar]
  13. RANDALL R. E., YOUNG D. F., GOSWAMI K. K. A., RUSSELL W. C. 1987; Isolation and characterization of monoclonal antibodies to simian virus 5 and their use in revealing antigenic differences between human, canine and simian isolates. Journal of General Virology 68:2769–2780
     [Google Scholar]
  14. WAXHAM M. N., SERVER A. C, GOODMAN H. M., WOLINSKY J. S. 1987; Cloning and sequencing of the mumps virus fusion protein gene. Virology 159:381–388
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-8-2051
Loading
Comparison between Parainfluenza Virus Type 2 and Simian Virus 5: Monoclonal Antibodies Reveal Major Antigenic Differences
J. Gen. Virol. 69, 2051 (1988); https://doi.org/10.1099/0022-1317-69-8-2051
/content/journal/jgv/10.1099/0022-1317-69-8-2051
/content/journal/jgv/10.1099/0022-1317-69-8-2051
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error