1887

Abstract

Summary

Two monoclonal antibodies (MAbs) with molecular specificities for either the viral envelope glycoprotein (MAb 541) or the non-structural NS1 glycoprotein (MAb 109) were derived using West Nile and yellow fever (YF) viruses respectively. Their antigenic reactivity with a large number of flaviviruses was tested by indirect immunofluorescence microscopy. Both produced cytoplasmic fluorescent staining patterns with the homologous virus against which they were raised. Additionally, MAb 541 reacted with two substrains of YF virus whereas MAb 109 reacted with Bussuquara, YF and Ntaya viruses. These reactions were exclusively cytoplasmic. Two unexpected patterns of fluorescent labelling were observed when the antibodies were tested with Zika and Langat viruses. MAb 541 produced fluorescent staining of the nuclei, but not the cytoplasm, of cells infected with Zika virus and MAb 109 labelled only the nucleoli of cells infected with Langat virus. Double-labelling experiments showed that the nuclear fluorescent label was confined to virus-infected cells, and antibody absorption experiments with virus-infected cell packs confirmed the virus specificity of the nuclear antigen. The unexpected presence of virus-specific antigen in the nuclei or nucleoli of Zika or Langat virus-infected cells brings into question the role of the nucleus in flavivirus replication.

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/content/journal/jgv/10.1099/0022-1317-69-8-1913
1988-08-01
2022-01-26
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References

  1. BELL D. 1974; DNA synthesis in nuclear monolayers from BSC-1 cells infected with herpes virus. Nature, London 248:505–508
    [Google Scholar]
  2. BRINTON M. A. 1986 Replication of flaviviruses. Togaviridae and Flaviviridae327–374 Edited by Schlesinger S., Schlesinger M. J. New York & London: Plenum Press;
    [Google Scholar]
  3. CARDIFF R. D., RUSS S. B., BRANDT W. E., RUSSELL P. K. 1973; Cytological localization of dengue-2 antigens: an immunological study with ultrastructural correlation. Infection and Immunity 8:1017–1025
    [Google Scholar]
  4. CARDIFF R. D., LUND J. K. 1976; Distribution of dengue-2 antigens by electron immunocytochemistry. Infection and Immunity 13:1699–1709
    [Google Scholar]
  5. GOULD E. A., GHANAS A. C, BUCKLEY A., CLEGG J. C. S. 1983; Monoclonal IgM antibody to JE virus that can react with a nuclear antigen in mammalian cells. Infection and Immunity 41:774–779
    [Google Scholar]
  6. GOULD E. A., BUCKLEY A., CAMMACK N., BARRETT A. D. T., CLEGG J. C. S., ISHAK R., VARMA M. G. R. 1985a; Examination of the immunological relationships between flaviviruses using yellow fever virus monoclonal antibodies. Journal of General Virology 66:1369–1382
    [Google Scholar]
  7. GOULD E. A., BUCKLEY A., CAMMACK N. 1985b; Use of the biotin-streptavidin interaction to improve flavivirus detection by immunofluorescence and ELISA tests. Journal of Virological Methods 11:41–48
    [Google Scholar]
  8. MURPHY F. A. 1980 Morphology and morphogenesis. St Louis Encephalitis65–103 Edited by Monath T. P. Washington: American Public Health Association;
    [Google Scholar]
  9. PFEFFERKORN E. R., SHAPIRO D. 1974 Reproduction of Togavirus. Comprehensive Virology 3171–230 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  10. TIKHOMIROVA T. I., KARPOVICH L. G., REINGOLD V. N., LEVKOVICH E. N., SHESTOPALOVA N. M. 1968; Comparative study of Langat and tick-borne encephalitis viruses in cultured cells. Acta virologica 12:529–534
    [Google Scholar]
  11. VARMA M. G. R., PUDNEY M., LEAKE C. J. 1973; Cell lines from larvae of Aedes (Stegomyia) malayensis (Colless) and Aedes (S.) pseudoscutellaris (Theobald) and their infection with some arboviruses. Transactions of the Royal Society of Tropical Medicine and Hygiene 68:374–382
    [Google Scholar]
  12. WESTAWAY E. G. 1980 Replication of flaviviruses. The Togaviruses531–577 Edited by Schlesinger R. W. New York & London: Academic Press;
    [Google Scholar]
  13. WESTAWAY E. G., BRINTON M. A., GAIDAMOVICH S. Y. A., HORZINEK M. C, IGARASHI A., KÄÄRIÄINEN L., LVOV D. K., PORTERFIELD J. S., RUSSELL P. K., TRENT D. W. 1985; Flaviviridae. Intervirology 24:183–192
    [Google Scholar]
  14. YASUZUMI G., TSUBO I. 1965; Analysis of the development of Japanese B encephalitis (JBE) virus. III. Electron microscope studies on inclusion bodies appearing in neurons and microglial cells infected with JBE virus. Journal of Ultrastructurel Research 12:317–327
    [Google Scholar]
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