1887

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Volume 69, Issue 8

The IgG Subclass Responses Induced by Wild-type, Cold-adapted and Purified Haemagglutinin from Influenza Virus A/Queensland/6/72 in CBA/CaH Mice Free

Abstract

Summary

The IgG subclass and IgA responses were investigated in CBA/CaH mice after inoculation with wild-type (wt) and cold-adapted (ca) derivatives of influenza A/Queensland/6/72 virus, and with purified haemagglutinin (H3) derived from the wt strain of the same virus. Intranasal inoculation of the wt and ca viruses resulted in responses dominated by IgG2a in serum, saliva and lung secretions, whereas an intramuscular injection of purified H3 elicited the production of all four IgG subclasses in serum and IgG2b and IgG3 in saliva and lung secretions. The source of IgG on mucosal surfaces was from local production and was not a transudate from serum, as demonstrated by the lack of albumin in saliva and lung secretions, and by the appearance in saliva and lung samples of IgG subclasses not present in serum at the time of sampling. The level of IgA on mucosal surfaces was influenced by the growth restrictions of intranasally inoculated ca virus, resulting in higher levels of IgA in saliva, whereas wt virus, able to replicate at higher temperatures, induced higher levels of IgA in lung secretions. The purified H3 inoculated by the intramuscular route elicited lower levels of IgA in serum, saliva and lung secretions than either the wt or ca viruses.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cold-adapted , IgG subclasses and influenza A virus
© Society for General Microbiology 1988
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1988-08-01
2024-04-20
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References

  1. ADA G. L., JONES P. D. 1986; The immune response to influenza infection. Current Topics in Microbiology and Immunology 128:1–54
     [Google Scholar]
  2. BURK R. R., ESCHENBRUCH M., LEUTHARD P., STECK G. 1983; Sensitive detection of proteins and peptides in Polyacrylamide gels after formaldehyde fixation. Methods in Enzymology 91:247–254
     [Google Scholar]
  3. BURTON D. R. 1985; Review: immunoglobulin G: functional sites. Molecular Immunology 22:161–206
     [Google Scholar]
  4. CLEMENTS M. L., MURPHY B. R. 1986; Development and persistence of local and systemic antibody responses in adults given live-attenuated or inactivated influenza A virus vaccine. Journal of Clinical Microbiology 23:66–72
     [Google Scholar]
  5. COCKRELL R. M., MONSTRATOS A. 1982; Concentration of influenza virus using polyethylene glycol. Acta virologica 26:292–294
     [Google Scholar]
  6. COUTELIER J.-P., VAN DER LOGT J. T. M., HEESSEN F. W. A., WARNIER G., VAN SNICK J. 1987; IgG2a restriction of murine antibodies elicited by viral infections. Journal of Experimental Medicine 165:64–69
     [Google Scholar]
  7. CUATRECASAS P., ILLIANO G. 1971; Purification of neuraminidase from Vibrio cholerae, Clostridium perfringens and influenza virus by affinity chromatography. Biochemical and Biophysical Research Communications 44:178–184
     [Google Scholar]
  8. EV P. L., PROWSE S. J., JENKIN C. R. 1978; Isolation of pure IgG1, IgG2a, and IgG2b immunoglobulins from mouse serum using Protein A-Sepharose. Immunochemistry 15:429–436
     [Google Scholar]
  9. EZEKOWITZ R. A. B., BAMPTON M., GORDON S. 1983; Macrophage activation selectively enhances expression of Fc receptors for IgG2a. Journal of Experimental Medicine 157:807–812
     [Google Scholar]
  10. FAHEY J. L., WUNDERLICH J., MISHELL R. 1964; The immunoglobulins of mice. 1. Four major classes of immunoglobulins: 7S gamma 2-, 7S gamma 1-, gamma 1A (beta 2A)-, and 18S gamma 1M-globulins. Journal of Experimental Medicine 120:223–242
     [Google Scholar]
  11. FREESTONE D. S., HAMILTON-SMITH S., SCHILD G. C, BUCKLAND R., CHINN S., TYRRELL D. A. J. 1972; Antibody responses and resistance to challenge in volunteers vaccinated with live attenuated, detergent split and oil adjuvant A2/Hong Kong/68 (H3N2) influenza vaccines. Journal of Hygiene 70:531–543
     [Google Scholar]
  12. FUJISAWA H., TSURU S., TANIGUCHI M., ZINNAKA Y., NOMOTO K. 1987; Protective mechanisms against pulmonary infection with influenza virus. I. Relative contribution of polymorphonuclear leukocytes and of alveolar macrophages to protection during the early phase of intranasal infection. Journal of General Virology 68:425–432
     [Google Scholar]
  13. HASHIMOTO O., WRIGHT P. T., KARZON D. T. 1983; Antibody-dependent cell-mediated cytotoxicity against influenza virus-infected cells. Journal of Infectious Diseases 148:785–794
     [Google Scholar]
  14. HICKS J. T., ENNIS F. A., KIM E., VERBONITZ M. 1978; The importance of an intact complement pathway in recovery from a primary viral infection: influenza in decomplemented and in C5-deficient mice. Journal of Immunology 121:1437–1444
     [Google Scholar]
  15. HOLLOWAY P. W. 1973; A simple procedure for removal of Triton X-100 from protein samples. Analytical Biochemistry 53:403–408
     [Google Scholar]
  16. HOLT P. G., REID M., BRITTEN D., SEDGWICK J., BAZIN H. 1987; Suppression of IgE responses by passive antigen inhalation: dissociation of local (mucosal) and systemic immunity. Cellular Immunology 104:434–439
     [Google Scholar]
  17. JONES P. D., ADA G. L. 1986; Influenza virus specific antibody-secreting cells in the murine lung during primary influenza infection. Journal of Virology 60:614–619
     [Google Scholar]
  18. LEMERCIER G., NEVEU D., MOREL J., FONTANGES R. 1977; Local immune response in mouse experimental airborne influenza: immunoglobulin concentrations, antibody levels of immunoglobulin classes and anamnestic response in bronchial washings. Archives of Virology 53:45–53
     [Google Scholar]
  19. LIEW F. Y., RUSSELL S. M., APPLEYARD G., BRAND C. M., BEALE J. 1984; Cross-protection in mice influenza A virus by the respiratory route is correlated with local IgA antibody rather than serum antibody or cytotoxic T cell reactivity. European Journal of Immunology 14:350–356
     [Google Scholar]
  20. LOPEZ A. F., STRATH M., SANDERSON C. J. 1983; Mouse immunoglobulin isotypes mediating cytotoxicity of target cells by eosinophils and neutrophils. Immunology 48:503–509
     [Google Scholar]
  21. MAASSAB H. F. 1967; Adaptation and growth characteristics of influenza virus at 25 °C. Nature, London 213:612–614
     [Google Scholar]
  22. MCDERMOTT M. R., BEFUS A. D., BIENENSTOCK J. 1982; The structural basis for immunity in the respiratory tract. International Review of Experimental Pathology 23:47–112
     [Google Scholar]
  23. MACKENZIE J. S. 1977; The potential advantages and requirements of live attenuated influenza virus vaccines. Australian and New Zealand Journal of Medicine 7:431–437
     [Google Scholar]
  24. MACKENZIE J. S., MACKENZIE I., LLOYD J., DENT V. 1975; Comparative trials of live attenuated and detergent split influenza virus vaccines. Journal of Hygiene 75:425–443
     [Google Scholar]
  25. MAK N. K., SCHILTKNECHT E., ADA G. L. 1983; Protection of mice against influenza infection: enhancement of non-specific cellular responses by Corynebacterium parvum. Cellular Immunology 78:314–325
     [Google Scholar]
  26. NAI-KI M., YONG-HE Z., ADA G. L., TANNOCK G. A. 1982; Humoral and cellular responses of mice to infection with a cold-adapted influenza A virus variant. Infection and Immunity 38:218–225
     [Google Scholar]
  27. NATUK R. J., WELSH R. M. 1987; Accumulation and Chemotaxis of natural killer/large granular lymphocytes at sites of virus replication. Journal of Immunology 138:877–883
     [Google Scholar]
  28. O’FARRELL P. H. 1974; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
     [Google Scholar]
  29. OI V. T., VUONG T. M., HARDY R., REIDLER J., DONGL J., HENZENBERG L. A., STRYER L. 1984; Correlation between segmental flexibility and effector functions of antibodies. Nature, London 307:136–140
     [Google Scholar]
  30. OWENS S. L., OSEBOLD J. W., ZEE Y. C. 1981; Dynamics of B-lymphocytes in the lungs of mice exposed to aerosolised influenza virus. Infection and Immunity 33:231–238
     [Google Scholar]
  31. PHELAN M. A., MAYNER R. E., BÛCHER D. J., ENNIS F. A. 1980; Purification of influenza virus glycoproteins for the preparation and standardization of immunological potency testing reagents. Journal of Biological Standardization 8:233–242
     [Google Scholar]
  32. REALE M. A., BONA C. A., SCHULMAN J. L. 1985; Isotype profiles of anti-influenza antibodies in mice bearing the xid defect. Journal of Virology 53:425–429
     [Google Scholar]
  33. RODBARD D., HUTT D. M. 1974 Statistical analysis of radioimmunoassays and immunoradiometric (labelled antibody) assays: generalised, weighted, iterative least squares method for logistic curve fitting. Proceedings of the Symposium on Radioimmunoassay and Related Procedures in Medicine165–192 New York: International Atomic Energy Agency;
     [Google Scholar]
  34. RODGERS B. C., MIMS C. A. 1981; Interaction of influenza virus with mouse macrophages. Infection and Immunity 31:751–757
     [Google Scholar]
  35. RODGERS B. C., MIMS C. A. 1982; Role of macrophage activation and interferon in the resistance of alveolar macrophages from infected mice to influenza virus. Infection and Immunity 36:1154–1159
     [Google Scholar]
  36. SCHULMAN J. L. 1975 Immunology of influenza. The Influenza Viruses and Influenza373–393 Edited by Kilbourne E. D. New York & London: Academic Press;
     [Google Scholar]
  37. SLACK J., DER-BALIAN G. P., NAHM M., DAVIE J. M. 1980; Subclass restriction of murine antibodies. II. The IgG plaque-forming cell response to thymus-independent type 1 and type 2 antigens in normal mice and mice expressing an X-linked immunodeficiency. Journal of Experimental Medicine 151:853–862
     [Google Scholar]
  38. SPIELBERG H. L. 1974; Biological activities of immunoglobulins of different classes and subclasses. Advances in Immunology 19:259–294
     [Google Scholar]
  39. TANNOCK G. A., PAUL A. P. 1987; Homotypic and heterotypic immunity of influenza A viruses induced by recombinants of the cold-adapted master strain A/Ann Arbor/6/60-ca. Archives of Virology 92:121–133
     [Google Scholar]
  40. TANNOCK G. A., PAUL J. A., BARRY R. D. 1984; Relative immunogenicity of the cold-adapted influenza virus A/Ann Arbor/6/60 (A/AA/6/60-ca), recombinants of A/AA/6/60-ca, and parental strains with similar surface antigens. Infection and Immunity 43:457–462
     [Google Scholar]
  41. TAYLOR H. P., DIMMOCK N. J. 1985a; Mechanism of neutralization of influenza virus by secretory IgA is different from that of monomeric IgA or IgG. Journal of Experimental Medicine 161:198–209
     [Google Scholar]
  42. TAYLOR H. P., DIMMOCK N. J. 1985b; Mechanisms of neutralization of influenza virus by IgM. Journal of General Virology 66:903–907
     [Google Scholar]
  43. TSURU S., FUJISAWA H., TANIGUCHI M., ZINNAKA Y., NOMOTO K. 1987; Mechanism of protection during the early phase of a generalized viral infection. II. Contribution of polymorphonuclear leukocytes to protection against intravenous infection with influenza virus. Journal of General Virology 68:419–424
     [Google Scholar]
  44. VIRELIZIER J. L. 1975; Host defences against influenza virus: the role of anti-hemagglutinin antibody. Journal of Immunology 115:434–439
     [Google Scholar]
  45. VIRELIZIER J. L., ALLISON A. C, SCHILD G. C. 1979; Immune responses to influenza in the mouse and their control of the infection. British Medical Bulletin 35:65–68
     [Google Scholar]
  46. VOLLER A., BIDWELL D., BARTLETT A. 1976 Microplate enzyme immunoassays for the immunodiagnosis of virus infections. Manual of Clinical Immunology506–512 Edited by Rose N. R., Friedman H. Washington, D.C.: American Society for Microbiology;
     [Google Scholar]
  47. WAGNER D. K., CLEMENTS M. L., REIMER C. B., SYNDER M., NELSON D. L., MURPHY B. R. 1987; Analysis of immunoglobulin G antibody responses after administration of live and inactivated influenza A vaccine indicates that nasal wash immunoglobulin G is a transudate from serum. Journal of Clinical Microbiology 25:559–562
     [Google Scholar]
  48. WEBSTER R. G., ASKONAS B.A. 1980; Cross-protection and cross-reactive cytotoxic T cells induced by influenza virus vaccines in mice. European Journal of Immunology 10:396–401
     [Google Scholar]
  49. WINKELHAKE J. L. 1978; Review: immunoglobulin structure and effector functions. Immunochemistry 15:695–714
     [Google Scholar]
  50. YAP K. L., BRACIALE J., ADA G. L. 1979; Role of T-cell function in recovery from murine influenza infection. Cellular Immunology 43:341–351
     [Google Scholar]
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The IgG Subclass Responses Induced by Wild-type, Cold-adapted and Purified Haemagglutinin from Influenza Virus A/Queensland/6/72 in CBA/CaH Mice
J. Gen. Virol. 69, 1873 (1988); https://doi.org/10.1099/0022-1317-69-8-1873
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