1887

Abstract

Summary

Exudation of polymorphonuclear leukocytes (PMN) from the infected mucosa is a characteristic feature of influenza virus infection. Since reactive oxygen species generated by PMN can be strong mutagens, the possibility of production of antigenic variants of the virus by virus-PMN interaction was investigated. Cloned influenza A NWS (H1N1) virus multiplying in Madin-Darby canine kidney cells was treated with human peripheral PMN. Assays in the presence and absence of monoclonal antibody to the cloned virus showed a seven- to ten-fold increase in the frequency of variants in the presence of PMN. The mutagenic effect was abolished by addition of superoxide dismutase to the culture.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-8-1841
1988-08-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/8/JV0690081841.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-8-1841&mimeType=html&fmt=ahah

References

  1. BIRNBOIM H. C. 1982; DNA strand breakage in human leukocytes exposed to a tumor promoter, phorbol myristate acetate. Science 215:1247–1249
    [Google Scholar]
  2. BUONAGURIO D. A., NAKADA S., PARVIN J. D., CRYSTAL M., PALESE P., FITCH W. M. 1986; Evolution of human influenza A viruses over 50 years: rapid, uniform rate of change in NS gene. Science 232:980–982
    [Google Scholar]
  3. CAMPBELL R. C. 1967 Statistics for Biologists Cambridge: Cambridge University Press;
    [Google Scholar]
  4. DOWDLE W. R., COLEMAN M. T. 1974 Influenza virus. Manual of Clinical Microbiology, 2nd edn.678–685 Edited by Lennette E. H., Spaulding E. H., Truant J. P. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  5. FARR S. B., D’ARI R., TOUATI D. 1986; Oxygen-dependent mutagenesis in Escherichia coli lacking superoxide dismutase. Proceedings of the National Academy of Sciences, U.S.A 83:8268–8272
    [Google Scholar]
  6. MATSUYAMA T., SWEET C, COLLIE M. H., SMITH H. 1980; Aspects of virulence in ferrets exhibited by influenza virus recombinants of known genetic constitution. Journal of Infectious Diseases 141:351–361
    [Google Scholar]
  7. MILLS E. L., DEBETS-OSSENKOPP Y., VERBRUGH H. A., VERHOEF J. 1981; Initiation of the respiratory burst of human neutrophils by influenza virus. Infection and Immunity 32:1200–1205
    [Google Scholar]
  8. PORTNER A., WEBSTER R. G., BEAN W. J. 1980; Similar frequencies of antigenic variants in Sendai, vesicular stomatitis, and influenza A viruses. Virology 104:235–238
    [Google Scholar]
  9. REPINE J. E., PFENNINGER O. W., TALMAGE D. W., BERGER E. M., PETTIJOHN D. E. 1981; Dimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or ion/hydrogen peroxide-generated hydroxyl radical. Proceedings of the National Academy of Sciences, U.S.A 78:1001–1003
    [Google Scholar]
  10. SWEET C., SMITH H. 1980; Pathogenicity of influenza virus. Microbiological Reviews 44:303–330
    [Google Scholar]
  11. SWEET C, BIRD R. A., CAVANAGH D., TOMS G. L., COLLIE M. H., SMITH H. 1979; The local origin of the febrile response induced in ferrets during respiratory infection with a virulent influenza virus. British Journal of Experimental Pathology 60:300–308
    [Google Scholar]
  12. TOBITA K., SUGIURA A., ENOMOTO C., FURUYAMA M. 1975; Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Medical Microbiology and Immunology 162:9–14
    [Google Scholar]
  13. TOMS G. L., DAVIES J. A., WOODWARD C. G., SWEET C., SMITH H. 1977; The relation of pyrexia and nasal inflammatory response to virus levels in nasal washings of ferrets infected with influenza viruses of differing virulence. British Journal of Experimental Pathology 58:444–458
    [Google Scholar]
  14. WEITBERG A. B., WEITZMAN S. A., DESTREMPES M., LATT S. A., STOSSEL T. P. 1983; Stimulated human phagocytes produce cytogenetic changes in cultured mammalian cells. New England Journal of Medicine 308:26–30
    [Google Scholar]
  15. WEITZMAN S. A., STOSSEL T. P. 1981; Mutation caused by human phagocytes. Science 212:546–547
    [Google Scholar]
  16. WEITZMAN S. A., STOSSEL T. P. 1982; Effects of oxygen radical scavengers and antioxidants on phagocyte-induced mutagenesis. Journal of Immunology 128:2770–2772
    [Google Scholar]
  17. WEITZMAN S. A., WEITBERG A. B., CLARK E. P., STOSSEL T. P. 1985; Phagocytes as carcinogens: malignant transformation produced by human neutrophils. Science 227:1231–1233
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-69-8-1841
Loading
/content/journal/jgv/10.1099/0022-1317-69-8-1841
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error