Exudation of polymorphonuclear leukocytes (PMN) from the infected mucosa is a characteristic feature of influenza virus infection. Since reactive oxygen species generated by PMN can be strong mutagens, the possibility of production of antigenic variants of the virus by virus-PMN interaction was investigated. Cloned influenza A NWS (H1N1) virus multiplying in Madin-Darby canine kidney cells was treated with human peripheral PMN. Assays in the presence and absence of monoclonal antibody to the cloned virus showed a seven- to ten-fold increase in the frequency of variants in the presence of PMN. The mutagenic effect was abolished by addition of superoxide dismutase to the culture.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error