1887

Abstract

Summary

Particles of tobacco ringspot nepovirus from purified preparations were trapped on grids by immunosorbent electron microscopy and then either negatively stained, or freeze-dried and shadowed with uranium, for structural studies. Particle dimensions differed considerably with different stains and methods of preparation, but no obvious substructure was apparent. In contrast, particles which were freeze-dried and then shadowed exhibited either fivefold or threefold symmetry and had a structure resembling that of models made up of 60 units in clusters of five arranged in a T = 1 lattice. Both chemical and morphological evidence are compatible with this structure.

Keyword(s): electron microscopy , structure and TRSV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-8-1831
1988-08-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/8/JV0690081831.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-8-1831&mimeType=html&fmt=ahah

References

  1. ADOLPH K. W., BUTLER P. J. G. 1974; Studies on the assembly of a spherical plant virus. I. States of aggregation of the isolated protein. Journal of Molecular Biology 88:327–341
    [Google Scholar]
  2. CHAMBERS T. C, FRANCKI R. I. B., RANDLES J. W. 1965; The fine structure of gladiolus virus. Virology 25:15–21
    [Google Scholar]
  3. CHU P. W. G., FRANCKI R. I. B. 1979; The chemical subunit of tobacco ringspot virus coat protein. Virology 93:398–412
    [Google Scholar]
  4. CROWTHER R. A., GEELEN J. L. M. C., MELLEMA J. E. 1974; A three dimensional image reconstruction of cowpea mosaic virus. Virology 57:20–27
    [Google Scholar]
  5. DUNCAN G. H., ROBINSON D. J. 1981; Effects of specimen preparation on measurements of virus particles. Annual Report of Scottish Horticultural Research Institute, 1980107–108
    [Google Scholar]
  6. FABERGÉ A. C., OLIVER R. M. 1974; Methylamine tungstate, a new negative stain. Journale de Microscopie 20:241–246
    [Google Scholar]
  7. FINCH J. T., KLUG A. 1966; Arrangement of protein subunits and the distribution of nucleic acid in turnip yellow mosaic virus. II. Electron microscopic studies. Journal of Molecular Biology 15:344–364
    [Google Scholar]
  8. FINCH J. T., KLUG A., LEBERMAN R. 1970; The structure of turnip crinkle and tomato bushy stunt viruses. II. The surface structure: dimer clustering patterns. Journal of Molecular Biology 50:215–222
    [Google Scholar]
  9. FINCH J. T., CROWTHER R. A., HENDRY D. A., STRUTHERS J. K. 1974; The structure of Nudaurelia capensis B virus: the first example of a capsid with icosahedral surface symmetry T = 4. Journal of General Virology 24:191–200
    [Google Scholar]
  10. HALL C. E., MACLEAN E. C., TESSMAN I. 1959; Structure and dimensions of bacteriophage ϕX174 from electron microscopy. Journal of Molecular Biology 1:192–194
    [Google Scholar]
  11. HÖRNE R. W. 1961 The examination of small particles. Techniques in Electron Microscopy311–355 Edited by Kay D. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  12. HORNE R. W. 1979; The formation of virus crystalline and paracrystalline arrays for electron microscopy and image analysis. Advances in Virus Research 24:173–221
    [Google Scholar]
  13. HORNE R. W., WILDY P. 1961; Symmetry in virus architecture. Virology 15:348–373
    [Google Scholar]
  14. MAYO M. A., MURANT A. F., HARRISON B. D. 1971; New evidence on the structure of nepoviruses. Journal of General Virology 12:175–178
    [Google Scholar]
  15. MAYO M. A., BARKER H., HARRISON B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  16. ROBERTS I. M. 1977; Negative staining in electron microscope serology. Micron 8:215–216
    [Google Scholar]
  17. ROBERTS I. M. 1982; Examination and printing of micrographs using a microfiche reader. Annual Report of the Scottish Crop Research Institute, 1981196
    [Google Scholar]
  18. ROBERTS I. M. 1984 Freeze-drying and high resolution shadowing of plant virus particles for structural studies. Proceedings of the Eighth European Congress on Electron Microscopy 31805–1806 Budapest: Hungary;
    [Google Scholar]
  19. ROBERTS I. M. 1986a Practical aspects of handling, preparing and staining samples containing plant virus particles for electron microscopy. Developments and Applications in Virus Testing213–243 Edited by Jones R. A. C., Torrance L. Wellesbourne: Association of Applied Biologists;
    [Google Scholar]
  20. ROBERTS I. M. 1986b Immunoelectron microscopy of extracts of virus-infected plants. Electron Microscopy of Proteins 5 Viral Structure 294–357 Edited by Harris J. R., Horne R. W. Orlando & London: Academic Press;
    [Google Scholar]
  21. ROBERTS I. M., DUNCAN G. H. 1981; A simple device for freeze-drying electron microscope specimens. Journal of Microscopy 124:295–303
    [Google Scholar]
  22. ROBERTS I. M., HARRISON B. D. 1979; Detection of potato leafroll and potato mop-top viruses by immunosorbent electron microscopy. Annals of Applied Biology 93:289–297
    [Google Scholar]
  23. ROBERTS I. M., MAYO M. A. 1980; Electron microscope studies of the structure of the disk aggregate of tobacco rattle virus protein. Journal of Ultrastructure Research 71:49–59
    [Google Scholar]
  24. STACE-SMITH R. 1985; Tobacco ringspot virus. AAB Descriptions of Plant Viruses 309:
    [Google Scholar]
  25. WRIGLEY N. G. 1968; The lattice spacing of crystalline catalase as ah internal standard of length in electron microscopy. Journal of Ultrastructure Research 24:454–464
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-8-1831
Loading
/content/journal/jgv/10.1099/0022-1317-69-8-1831
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error