1887

Abstract

Summary

A panel of mouse monoclonal antibodies (MAbs) against potato virus X (PVX) was obtained and three of these which had high affinity to the antigen were characterized in detail. These three antibodies defined two epitopes on PVX and recognized native virus, viral coat protein and denatured viral coat protein in various immunological assays. Two of the MAbs and rabbit anti-PVX polyclonal antibodies bound to the 68 amino acid N-terminal peptide of the PVX coat protein. This implies that the N terminus of the PVX coat protein is exposed at the virus surface and forms a highly immunogenic antigenic determinant. In double antibody sandwich (DAS) ELISA, MAbs and their horseradish peroxidase conjugates reacted with PVX at 10 to 20 ng/ml. Monoclonal antibodies to PVX reacted with virus in potato leaves and tubers and detected the virus in DAS ELISA in various combinations, including in combination with polyclonal antibodies.

Keyword(s): antigenic determinants , MAbs and PVX
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1988-08-01
2021-09-24
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References

  1. AL MOUDALLAL Z., BRIAND J. P., VAN REGENMORTEL M. H. V. 1982; Monoclonal antibodies as probes of the antigenic structure of tobacco mosaic virus. EMBO Journal 1:1005–1010
    [Google Scholar]
  2. BANTTARI E. E., GOODWIN B. H. 1985; Detection of potato viruses S, X and Y by enzyme-linked immunosorbent assay on nitrocellulose membrane (Dot-ELISA). Plant Disease 69:202–205
    [Google Scholar]
  3. BOBKOVA A. F., NACVLISHVILI N. M., SOROKINA N. B., EGOROV A. M., ATABEKOV J. G. 1983; Application of immunoferment method to diagnosis of potato X-virus diseases in potato plants and tubers. Sel’sko-plant viruses. Doklady VASHNIL 12:15–18 in Russian
    [Google Scholar]
  4. BURGERMEISTER W., KOENIG R. 1984; Electro-blot immunoassay - a means for studying serological relationships among plant viruses?. Phytopathologische Zeitschrift 111:15–25
    [Google Scholar]
  5. CLARK M. F., ADAMS A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  6. CLARK M. F., LISTER P. M., BAR-JOSEPH M. 1986; ELISA techniques. Methods in Enzymology 111:742–766
    [Google Scholar]
  7. FRIQUET B., CHAFFOTTE A. F., DJAVADI-OHANIANCE L., GOLDBERG M. E. 1985; Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. Journal of Immunological Methods 77:305–319
    [Google Scholar]
  8. GOODWIN P. H., BANTTARI E. E. 1984; Increased sensitivity of ELISA for potato viruses S, X, and Y by polystyrene pretreatments, additives, and a modified assay procedure. Plant Disease 68:944–947
    [Google Scholar]
  9. GUGERLI P., FRIES P. 1983; Characterization of monoclonal antibodies to potato virus Y and their use for virus detection. Journal of General Virology 64:2471–2477
    [Google Scholar]
  10. HALK E. L., DE BOER S. H. 1985; Monoclonal antibodies in plant disease research. Annual Review of Phytopathology 23:321–350
    [Google Scholar]
  11. HAWKES R., NIDAY E., GORDON J. 1982; A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119:142–147
    [Google Scholar]
  12. JÄRVEKÜLG L., SOBER J., SINUÄRV R., SAARMA M. 1987; Use of time-resolved fluoroimmunoassay for testing of plant viruses. Doklady VASHNIL 12:15–18 in Russian
    [Google Scholar]
  13. KOENIG R. 1972; Anomalous behavior of the coat proteins of potato virus X and cactus virus X during electrophoresis in dodecylsulfate-containing Polyacrylamide gels. Virology 50:263–266
    [Google Scholar]
  14. KOENIG R. 1978; ELISA in the study of homologous and heterologous reactions of plant viruses. Journal of General Virology 40:309–318
    [Google Scholar]
  15. KOENIG R., TORRANCE L. 1986; Antigenic analysis of potato virus X by means of monoclonal antibodies. Journal of General Virology 67:2145–2151
    [Google Scholar]
  16. KOENIG R., TREMAINE J. H., SHEPARD J. F. 1978; In situ degradation of the protein chain of potato virus X at the N- and C-termini. Journal of General Virology 38:329–337
    [Google Scholar]
  17. KURPPA A. 1983; Potato viruses in Finland and their identification. Journal of the Scientific Agricultural Society of Finland 55:183–301
    [Google Scholar]
  18. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  19. MASSALSKI P. R., HARRISON B. D. 1987; Properties of monoclonal antibodies to potato leafroll luteovirus and their use to distinguish virus isolates differing in aphid transmissibility. Journal of General Virology 68:1813–1821
    [Google Scholar]
  20. MOROZOV S. YU., ZAKHARIEV V. M., CHERNOV B. K., PRASOLOV V. S., KOZLOV YU. V., ATABEKOV J. G., SKRJABIN K. G. 1983; The primary structure and location of the coat protein gene in the genomic RNA of potato virus X. Doklady Akademii nauk SSSR 271:211–215 in Russian
    [Google Scholar]
  21. PURCIFULL D. E., EDWARDSON J. R. 1981 Handbook of Plant Virus Infections and Comparative Diagnosis943 Edited by Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  22. RADAVSKY J. L., GRAMA D. L., ZAIKIN A. A., WITER S. S., TUROVA I. P., BOBKOVA A. F., GOLDSTEIN M. I., JÄRVEKÜLG L. V., SOBER J. P., SAARMA M. J. 1988; The antigenic structure of potato virus X coat protein. I. The immunochemical analysis of cyanogen bromide fragments. Bioorganicheskaya Khimiya 14:20–26 in Russian
    [Google Scholar]
  23. RICH A. E. 1983 Potato Diseases New York & London: Academic Press;
    [Google Scholar]
  24. SAARMA M. J., NEUMAN T. E., TALPSEPP T. E., TOOTS I. E., SIIGUR E. P., SIIGUR J. P. 1984; Monoclonal antibodies against Vipera lebetina venom nerve growth factor. Doklady Akademii nauk SSSR 277:1267–1269 in Russian
    [Google Scholar]
  25. SHALLA T. A., SHEPARD J. F. 1970; An antigenic analysis of potato virus X and of its degraded protein. II. Evidence for a conformational change associated with the depolymerization of structural protein. Virology 42:835–847
    [Google Scholar]
  26. STOCKER J. W., FOSTER H. K., MIGGIANO V., STAHLI C, STAIGER G., TAKACS B., STAEHELIN T. 1982; Generation of two new myeloma cell lines “PAI” and “PAI-O” for hybridoma production. Research Disclosure 217:155–157
    [Google Scholar]
  27. TORRANCE L., LARKINS A. P., BUTCHER G. W. 1986; Characterization of monoclonal antibodies against potato virus X and comparison of serotypes with resistance groups. Journal of General Virology 67:57–67
    [Google Scholar]
  28. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of protein from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  29. TREMAINE J. H., MACKENZIE D. J., RONALD W. P. 1985; Monoclonal antibodies as structural probes of southern bean mosaic virus. Virology 144:80–87
    [Google Scholar]
  30. TUNG I. S., KNIGHT C A. 1972; The coat protein subunits of potato virus X and white clover mosaic virus. A comparison of methods for determining their molecular weights and some in situ degradation products of potato virus X protein. Virology 49:214–223
    [Google Scholar]
  31. WILSON M. B., NAKANE P. K. 1978 Developments in the periodate method of conjugating horseradish peroxidase to antibodies. Immunofluorescence and Related Staining Techniques215–244 Edited by Knapp W., Holubar K., Wick G. Amsterdam & New York: Elsevier/North-Holland;
    [Google Scholar]
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