1887

Abstract

Summary

Double-stranded cDNA of potato virus X (PVX) genomic RNA has been cloned and sequenced. The sequence [6435 nucleotides excluding the poly(A) tract] revealed five open reading frames (ORFs) which were numbered one to five starting at the 5′ terminus of the RNA. They encoded proteins of 165588 (166K), 24622 (25K), 12324 (12K), 7595 (8K) and 25080 (coat protein), respectively. ORFs 1 and 2 were inphase coding regions. The ORF 1 product contained domains of homology with the tobacco mosaic virus 126K and 183K products. The ORF 2 and 3 products showed homologies with the barley stripe mosaic virus 58K and 14K proteins, the beet necrotic yellow vein virus 42K and 13K products and the white clover mosaic virus 26K and 13K products, respectively. The significance of these homologies with respect to putative functions of the PVX-encoded proteins are discussed.

Keyword(s): nucleotide sequence , potexvirus and PVX
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-8-1789
1988-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/8/JV0690081789.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-8-1789&mimeType=html&fmt=ahah

References

  1. AHLQUIST P., STRAUSS E. C, RICE C. M., STRAUSS J. H., HASELOFF J., ZIMMERN D. 1985; Sindbis virus proteins nsPl and nsP2 contain homology to non-structural proteins from several RNA plant viruses. Journal of Virology 53:536–542
    [Google Scholar]
  2. BENDENA W. G., BANCROFT J. B., MACKIE G. A. 1987; Molecular cloning of clover yellow mosaic virus RNA: identification of coat protein encoding sequences in vivo and in vitro. Virology 157:276–284
    [Google Scholar]
  3. BERCKS R. 1970; Potato virus X. CMI/AAB Descriptions of Plant Viruses 4:
    [Google Scholar]
  4. BIGGIN M. D., GIBSON T. J., HONG G. F. 1983; Buffer gradient gels and [35S] label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A 80:3963–3965
    [Google Scholar]
  5. BOUZOUBAA S., ZIEGLER V., BECK D., GUILLEY H., RICHARDS K., JONARD G. 1986; Nucleotide sequence of beet necrotic yellow vein virus RNA-2. Journal of General Virology 67:1689–1700
    [Google Scholar]
  6. BOUZOUBAA S., QUILLET L., GUILLEY H., JONARD G., RICHARDS K. 1987; Nucleotide sequence of beet necrotic yellow vein virus RNA-1. Journal of General Virology 68:615–626
    [Google Scholar]
  7. CORNELISSEN B. J. C., BOL J. F. 1984; Homology between the proteins encoded by tobacco mosaic virus and two tricornaviruses. Plant Molecular Biology 3:379–384
    [Google Scholar]
  8. CORNELISSEN B. J. C., VAN VLOTEN-DOTING L. 1988 Molecular biology of RNA viruses. Cell Culture and Somatic Cell Genetics of Plants 7 Molecular Biology of Plant Nuclear Genes Edited by Schell J., Vasil I. K. New York & London: Academic Press; in press
    [Google Scholar]
  9. DAGERT M., EHRLICH S. D. 1979; Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
    [Google Scholar]
  10. DAVIS L. G., DIBNER M. D., BATTEY J. F. 1986 Basic Methods in Molecular Biology Amsterdam: Elsevier;
    [Google Scholar]
  11. DAVIS R. W., THOMAS H., CAMERON J., JOHN T. P. S., SCHERER S., PADGETT R. A. 1980; Rapid DNA isolations for enzymatic and hybridization analysis. Methods in Enzymology 65:404–411
    [Google Scholar]
  12. DOLJA V. V., GRAMA D. P., MOROZOV S. YU., ATABEKOV J. G. 1987; Potato virus X-related single- and double-stranded RNAs. Characterization and identification of terminal structures. FEBS Letters 214:308–312
    [Google Scholar]
  13. FORSTER R. L. S., GUILFORD P. J., FAULDS D. V. 1987; Characterization of the coat protein subgenomic RNA of white clover mosaic virus. Journal of General Virology 68:181–190
    [Google Scholar]
  14. FORSTER R. L. S., BEVAN M. W., HARBISON S.-A., GARDNER R. C. 1988; The complete nucleotide sequence of the potexvirus white clover mosaic virus. Nucleic Acids Research 16:291–303
    [Google Scholar]
  15. GUBLER U., HOFFMAN B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  16. GUILFORD P. J., FORSTER R. L. S. 1986; Detection of polyadenylated subgenomic RNAs in leaves infected with the potexvirus daphne virus X. Journal of General Virology 67:83–90
    [Google Scholar]
  17. GUSTAFSON G., ARMOUR S. L. 1986; The complete sequence of RNA β from the type strain of barley stripe mosaic virus. Nucleic Acids Research 14:3895–3909
    [Google Scholar]
  18. HARBISON S.-A., FORSTER R. L. S., GUILFORD P. J., GARDNER R. C. 1988; Organization and interviral homologies of the coat protein gene of white clover mosaic virus. Virology 162:459–465
    [Google Scholar]
  19. HASELOFF J., GOELET P., ZIMMERN D., AHLQUIST P., DASGUPTA R., KAESBERG P. 1984; Striking similarities in amino acid sequence among nonstructural proteins encoded by RNA viruses that have dissimilar genomic organization. Proceedings of the National Academy of Sciences, U.S.A 81:4358–4362
    [Google Scholar]
  20. HOLMES D. S., QUIGLEY M. 1981; Rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  21. JANDA M., FRENCH R., AHLQUIST P. 1987; High efficiency T7 polymerase synthesis of infectious RNA from cloned brome mosaic virus cDNA and effects of 5′ extensions on transcript infectivity. Virology 158:259–262
    [Google Scholar]
  22. KAMER G., ARGOS P. 1984; Primary structural comparison of RNA-dependent RNA polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  23. KIENY M. P., LATHE R., LECOCQ J. P. 1983; New versatile cloning and sequencing vectors based on bacteriophage M13. Gene 26:91–99
    [Google Scholar]
  24. LOK S., ABOUHAIDAR M. G. 1986; The nucleotide sequence of the 5′ end of papaya mosaic virus RNA: site of in vitro assembly initiation. Virology 153:289–296
    [Google Scholar]
  25. MESHI T., WATANABE Y., SAITO T., SUGIMOTO A., MAEDA T., OKADA Y. 1987; Function of the 30Kd protein of tobacco mosaic virus: involvement in cell-to-cell movement and dispensability for replication. EMBO Journal 6:2557–2563
    [Google Scholar]
  26. MOROZOV S. YU., ZAKCHARIEV V. M., CHERNOW B. K., PRASOLOV V. S., KOZLOV YU. V., ATABEKOV J. G., SKRYABIN K. G. 1983; The analysis of the primary structure and localization of the coat protein gene on the genomic RNA of potato virus X. DokladyAkademii nauk SSSR 271:211–215 in Russian
    [Google Scholar]
  27. MOROZOV S. YU., LUKASHEVA L. I., CHERNOV B. K., SKRYABIN K. G., ATABEKOV J. G. 1987; Nucleotide sequence of the open reading frames adjacent to the coat protein cistron in the potato virus X genome. FEBS Letters 213:438–442
    [Google Scholar]
  28. NISHIGUCHI M., MOTOYOSHI F., OSHIMA N. 1978; Behaviour of a temperature sensitive strain of tobacco mosaic virus in tomato leaves and protoplasts. Journal of General Virology 39:53–61
    [Google Scholar]
  29. NISHIGUCHI M., MOTOYOSHI F., OSHIMA N. 1980; Further investigation of a temperature sensitive strain of tobacco mosaic virus: its behaviour in tomato leaf epidermis. Journal of General Virology 46:497–500
    [Google Scholar]
  30. OHNO T., TAKAMATSU N., MESHI T., OKADA Y., NISHIGUCHI M., KIHO Y. 1983; Single amino acid substitution in 30K protein of TMV defective in virus transport function. Virology 131:255–258
    [Google Scholar]
  31. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  32. SAWYER L., TOLLIN P., WILSON H. R. 1987; A comparison between the predicted secondary structures of potato virus X and papaya mosaic virus coat proteins. Journal of General Virology 68:1229–1232
    [Google Scholar]
  33. SHORT M. N., DAVIES J. W. 1983; Narcissus mosaic virus: a potexvirus with an encapsidated subgenomic messenger RNA for coat protein. Bioscience Reports 3:837–846
    [Google Scholar]
  34. SHORT M. N., TURNER D. S., MARCH J. F., PAPPIN D. J. C, PARENTE A., DAVIES J. W. 1986; The primary structure of papaya mosaic virus coat protein. Virology 152:280–283
    [Google Scholar]
  35. SONENBERG N., SHATKIN A. J., RICCARDI R. P., RUBIN M., GOODMAN R. M. 1978; Analysis of terminal structures of RNA from potato virus X. Nucleic Acids Research 5:2501–2521
    [Google Scholar]
  36. STOLS A. L. H., HILL-VAN DER MEULEN G. W., TOEN M. K. I. 1970; Electron microscopy of Nicotianaglutinosa leaf cells infected with potato virus X. Virology 40:168–170
    [Google Scholar]
  37. TALIANSKY M. E., MALYSHENKO S. O., PSHENNIKOVA E. S., KAPLAN E. B., ULANOVA E. F., ATABEKOV J. G. 1982; Plant virus-specific transport function. I. Virus genetic control required for systemic spread. Virology 122:318–326
    [Google Scholar]
  38. WODNAR-FILIPOWICZ A., SKRZECZKOWSKI L. J., FILIPOWICZ W. 1980; Translation of potato virus X RNA into high molecular weight proteins. FEBS Letters 109:151–155
    [Google Scholar]
  39. ZIMMERN D. 1987 Evolution of RNA viruses. RNA Genetics Edited by Holland J., Domingo E., Ahlquist P. Boca Raton: CRC Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-8-1789
Loading
/content/journal/jgv/10.1099/0022-1317-69-8-1789
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error