1887

Abstract

Summary

Monoclonal antibodies (MAbs) against the major core protein p26 of equine infectious anaemia virus (EIAV) were produced and characterized. Sensitive enzyme-linked immunosorbent assay and Western blot immunoassay were employed to confirm the specificity of these MAbs. Western blot analysis also indicated that MAbs to p26 reacted with another EIAV protein of 55000 apparent (designated here as Pr55) present in density gradient-purified virus preparations. Rabbit antiserum prepared against p26 as well as MAbs to p26 detected Pr55 and several other intermediate cleavage products in detergent-soluble lysates of virus-infected cells in Western blot and immunoprecipitation assays. The results suggest that Pr55 is the polyprotein of EIAV.

Keyword(s): EIAV , monoclonal antibodies , p26 and protein
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/content/journal/jgv/10.1099/0022-1317-69-7-1719
1988-07-01
2022-01-23
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References

  1. BALL J. M., RAO V. S. V., ROBEY W. G., ISSEL C. J., MONTELARO R. C. 1988; Lentivirus antigen purification and characterization: isolation of equine infectious anemia virus gag and env proteins in one step by reverse phase HPLC and application to human immunodeficiency virus glycoproteins. Journal of Virological Methods 19:265–277
    [Google Scholar]
  2. COGGINS L., NORCROSS N. L. 1970; Immunodiffusion reaction in equine infectious anemia. Cornell Veterinarian 60:330–335
    [Google Scholar]
  3. HAHN B. H., SHAW G. M., TAYLOR M. E., REDFIELD R. R., MARKHAM P. D., SALAHUDDIN S. Z., WONG-STAAL F., GALLO R. C, PARKS E. S., PARKS W. P. 1986; Genetic variation in HTLV-III/LAV over time in patients with AIDS or at risk for AIDS. Science 232:1548–1553
    [Google Scholar]
  4. HENDERSON L. E., SOWDER R. C, SMYTHERS G. W., OROSZLAN S. 1987; Chemical and immunological characterizations of equine infectious anemia virus gag-encoded proteins. Journal of Virology 61:1116–1124
    [Google Scholar]
  5. HUSSAIN K. A., ISSEL C. J., SCHNORR K. L., RWAMBO P. M., MONTELARO R. C. 1987; Antigenic analysis of equine infectious anemia virus (EIAV) variants by using monoclonal antibodies: epitopes of glycoprotein gp90 of EIAV stimulate neutralizing antibodies. Journal of Virology 61:2956–2961
    [Google Scholar]
  6. ISSEL C. J., COGGINS L. 1979; Equine infectious anemia: current knowledge. Journal of the American Veterinary Medical Association 174:727–733
    [Google Scholar]
  7. ISSEL C. J., MONTELARO R. C., FOIL L. D. 1986 Virology of equine retroviruses. Animal Models of Retrovirus Infection and Their Relationship to AIDS95–105 Edited by Salzman L. A. Orlando & London: Academic Press;
    [Google Scholar]
  8. JAMJOOM G., KARSHIN W. L., NASO R. B., ARCEMENT L. J., ARLINGHAUS R. B. 1975; Proteins of Rauscher murine leukemia virus: resolution of a 70,000 dalton, nonglycosylated polypeptide containing p30 peptide sequences. Virology 68:135–145
    [Google Scholar]
  9. MONTELARO R. C, WEST M., ISSEL C. J. 1981; High-performance gel permeation chromatography of proteins in denaturing solvents and its application to the analysis of enveloped virus polypeptides. Analytical Biochemistry 114:398–406
    [Google Scholar]
  10. MONTELARO R. C, LOHREY N., PAREKH B., BLAKENEY E. W., ISSEL C. J. 1982; Isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus. Journal of Virology 42:1029–1038
    [Google Scholar]
  11. MONTELARO R. C, ISSEL C. J., PAYNE S., SALINOVICH O. 1986 Antigenic variation during persistent infections by equine infectious anaemia virus. Antigenic Variation In Infectious Diseases41–56 Edited by Birkbeck T. H., Penn C. W. Oxford & Washington D.C.: IRL Press;
    [Google Scholar]
  12. PAREKH B., ISSEL C. J., MONTELARO R. C. 1980; Equine infectious anemia virus, a putative lentivirus, contains polypeptides analogous to prototype-C oncornaviruses. Virology 107:520–525
    [Google Scholar]
  13. PAYNE S. L., SALINOVICH O., NAUMAN S. M., ISSEL C I., MONTELARO R. C. 1987; Course and extent of variation of equine infectious anemia virus during parallel persistent infections. Journal of Virology 61:1266–1270
    [Google Scholar]
  14. SALINOVICH O., PAYNE S. L., MONTELARO R. C, HUSSAIN K. A., ISSEL C J., SCHNORR K. L. 1986; Rapid emergence of novel antigenic and genetic variants of equine infectious anemia virus during persistent infection. Journal of Virology 57:71–80
    [Google Scholar]
  15. STEPHENS R. M., CASEY J. W., RICE L. R. 1986; Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus. Science 231:589–594
    [Google Scholar]
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