1887

Abstract

Summary

Five cosmids containing inserts that comprise the complete genome of the multicapsid nuclear polyhedrosis virus of were mapped with four restriction enzymes (II, I, I, I). From these cosmid maps, composite maps of the complete genome were constructed for each restriction enzyme. A region containing repeats of the sequence GGC downstream of the polyhedrin gene was used to probe the genome. It cross-hybridized with a region which, upon sequence analysis, was found to be a highly repetitive GC-rich region of nearly 500 nucleotides. The two GC-rich regions appeared to be evolutionarily unrelated.

Keyword(s): baculovirus , genome map and OpMNPV
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/content/journal/jgv/10.1099/0022-1317-69-6-1375
1988-06-01
2022-06-29
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References

  1. ANON 1980 M13 Cloning/Dideoxy Sequencing Manual Gaithersburg: Bethesda Research Laboratories;
    [Google Scholar]
  2. ARIF B., DOERFLER W. 1984; Identification and localization of reiterated sequences in the Choristoneura fumiferana MNPV genome. EMBO Journal 3:525–529
    [Google Scholar]
  3. BICKNELL J. N., LEISY D. J., ROHRMANN G. F., BEAUDREAU G. S. 1987; Comparison of the p26 gene region of two baculoviruses. Virology 161:589–592
    [Google Scholar]
  4. BURGESS S. 1977; Molecular weights of lepidopteran baculovirus DNAs: derivation by electron microscopy. Journal of General Virology 37:501–510
    [Google Scholar]
  5. COCHRAN M. A., FAULKNER P. 1983; Location of homologous DNA sequences interspersed at five regions in the baculovirus AcMNPV genome. Journal of Virology 45:961–970
    [Google Scholar]
  6. FLAVELL A. J., DYSON J., ISH-HOROWICZ D. 1987; A novel GC-rich dispersed repeat sequence in Drosophila melanogaster. Nucleic Acids Research 15:4035–4048
    [Google Scholar]
  7. GUARINO L. A., SUMMERS M. D. 1986; Interspersed homologous DNA of Autographa californica nuclear polyhedrosis virus enhances delayed early gene expression. Journal of Virology 60:215–223
    [Google Scholar]
  8. GUARINO L. A., GONZALEZ M. A., SUMMERS M. D. 1986; Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus. Journal of Virology 60:224–229
    [Google Scholar]
  9. HAYNES S. R., REBBERT M. L., MOZER B. A., FORQUIGNON F., DAWID I. B. 1987; pen repeat sequences are GGN clusters and encode a glycine-rich domain in a Drosophila cDNA homologous to the rat helix destabilizing protein. Proceedings of the National Academy of Sciences, U.S.A 84:1819–1823
    [Google Scholar]
  10. HENIKOFF S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  11. KNAUF V. C., NESTER E. W. 1982; Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti Plasmid. Plasmid 8:45–54
    [Google Scholar]
  12. KUZIO J., FAULKNER P. 1984; Regions of repeated DNA in the genome of Choristoneura fumiferana nuclear polyhedrosis virus. Virology 139:185–188
    [Google Scholar]
  13. LEISY D. J., ROHRMANN G. F., BEAUDREAU G. S. 1984; Conservation of genome organization in two multicapsid nuclear polyhedrosis viruses. Journal of Virology 52:699–702
    [Google Scholar]
  14. LEISY D., ROHRMANN G., BEAUDREAU G. 1986; The nucleotide sequence of the polyhedrin gene region of the multicapsid baculovirus of Orgyia pseudotsugata. Virology 153:280–288
    [Google Scholar]
  15. LIU A., QIN J., RANKIN C, HARDIN S. E., WEAVER R. F. 1986; Nucleotide sequence of a portion of the Autographa californica nuclear polyhedrosis virus genome containing the EcoRI site-rich region (hr5) and an open reading frame just 5′ to the plO gene. Journal of General Virology 67:2565–2570
    [Google Scholar]
  16. OELLIG C, HAPP B., MULLER T., DOERFLER W. 1987; Overlapping sets of viral RNAs reflect the array of polypeptides in the EcoRI J and N fragments (map positions 81·2 to 85·0) of the Autographa californica nuclear polyhedrosis virus. Journal of Virology 61:3048–3057
    [Google Scholar]
  17. PARKS C. L., JONES T. R., GONZALES I. L., SCHMICKEL R. D., HYMAN R. W., SPECTOR D. J. 1986; A simple repetitive sequence common to herpes simplex virus type 1 and human ribosomal DNAs. Virology 154:381–388
    [Google Scholar]
  18. PENNOCK G. D., SHOEMAKER C., MILLER L. K. 1984; Strong and regulated expression of Escherichia coli β-galactosidase in insect cells with a baculovirus vector. Molecular and Cellular Biology 4:399–406
    [Google Scholar]
  19. QUANT-RUSSELL R. L., PEARSON M. N., ROHRMANN G. F., BEAUDREAU G. S. 1987; Characterization of baculovirus plO synthesis using monoclonal antibodies. Virology 160:9–19
    [Google Scholar]
  20. ROHRMANN G. F., MARTIGNONI M. E., BEAUDREAU G. S. 1982; DNA sequence homology between Autographa californica and Orgyia pseudotsugata nuclear polyhedrosis viruses. Journal of General Virology 62:137–143
    [Google Scholar]
  21. SMITH G. E., SUMMERS M. D., FRASER M. J. 1983; Production of human beta interferon in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 3:2156–2165
    [Google Scholar]
  22. SPECTOR D. J., JONES T. R., PARKS C. L., DECKHUT A. M., HYMAN R. W. 1987; Hybridization between a repeated region of herpes simplex virus type 1 DNA containing the sequence [GGC]n and heterodisperse cellular DNA and RNA. Virus Research 7:69–82
    [Google Scholar]
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