1887

Abstract

Summary

Polyadenylated RNA was isolated from plants infected with tomato golden mosaic virus (TGMV). Northern hybridization with a strand-specific probe of cloned TGMV DNA A revealed a 0.9 kb transcript with the same orientation as virion DNA. The positions of the 5′ and 3′ termini of the transcript, which were mapped by nuclease protection and primer extension techniques, indicated that it corresponded to the virus coat protein mRNA and enabled the likely functional promoter and polyadenylation signals to be identified.

Keyword(s): coat protein gene , geminivirus and TGMV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-6-1359
1988-06-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/6/JV0690061359.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-6-1359&mimeType=html&fmt=ahah

References

  1. BAILEY J. M., DAVIDSON N. 1976; Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70:75–85
    [Google Scholar]
  2. BISARO D. M., HAMILTON W. D. O., COUTTS R. H. A., BUCK K. W. 1982; Molecular cloning and characterisation of the two DNA components of tomato golden mosaic virus. Nucleic Acids Research 10:4913–4922
    [Google Scholar]
  3. BREATHNACH R., CHAMBON P. 1981; Organisation and expression of eukaryotic split genes coding for proteins. Annual Review of Biochemistry 50:349–383
    [Google Scholar]
  4. CILIBERTO G., RANGEI G., COSTANZO F., DENTE L., CORTESE R. 1983; Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase III. Cell 32:725–733
    [Google Scholar]
  5. FAVALORO J., FREISMAN R., KAMEN R. 1980; Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods in Enzymology 65:718–749
    [Google Scholar]
  6. GAL A., NAHON J.-L., SALA-TREPAT J. M. 1983; Detection of rare mRNA species in a complex RNA population by blot hybridisation techniques. A comparative survey. Analytical Biochemistry 132:190–194
    [Google Scholar]
  7. HAMILTON W. D. O., SANDERS R. C, COUTTS R. H. A., BUCK K. W. 1981; Characterisation of tomato golden mosaic virus as a geminivirus. FEMS Microbiology Letters 11:263–267
    [Google Scholar]
  8. HAMILTON W. D. O., BISARO D. M., COUTTS R. H. A., BUCK K. W. 1983; Demonstration of the bipartite nature of the genome of a single-stranded DNA plant virus by infection with the cloned DNA components. Nucleic Acids Research 11:7387–7396
    [Google Scholar]
  9. HAMILTON W. D. O., STEIN V. E., COUTTS R. H. A., BUCK K. W. 1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus. Potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
    [Google Scholar]
  10. HAYES R. J., BROUGH C. L., PRINCE V. E., COUTTS R. H. A., BUCK K. W. 1988; Infection of Nicotiana benthamiana with uncut cloned tandem dimers of tomato golden mosaic virus DNA. Journal of General Virology 69:209–218
    [Google Scholar]
  11. HOWARTH A. J., CATON J., BOSSERT M., GOODMAN R. M. 1985; Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in gemini viruses. Proceedings of the National Academy of Sciences, U.S.A 82:3572–3576
    [Google Scholar]
  12. HU N., MESSING J. 1982; The making of strand-specific M13 probes. Gene 17:271–277
    [Google Scholar]
  13. JOSHI C. P. 1987; Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucleic Acids Research 15:9627–9640
    [Google Scholar]
  14. JOST J.-P., PEHLING G. 1976; Immunochemical isolation and characterisation of vitellogenin mRNA from liver of estradiol-treated chicks. European Journal of Biochemistry 66:339–346
    [Google Scholar]
  15. KALLENDER H., PETTY I. T. D., STEIN V. E., PANICO M., BLENCH I. P., ETIENNE A. T., MORRIS H. R., COUTTS R. H. A., BUCK K. W. 1988; Identification of the coat protein gene of tomato golden mosaic virus. Journal of General Virology 69:1351–1357
    [Google Scholar]
  16. KOZAK M. 1983; Comparison of initiation of protein synthesis in procaryotes, eucaryotes and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  17. KOZAK M. 1984; Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Research 12:857–872
    [Google Scholar]
  18. KOZAK M. 1987; An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Research 15:8125–8148
    [Google Scholar]
  19. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. MATTHEWS R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  21. MESSING J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–78
    [Google Scholar]
  22. MORINAGA T., IKEGAMI M., MIURA K. 1983; Infectivity of cloned DNAs from multiple components of bean golden mosaic virus. Proceedings of the Japan Academy B59:363–366
    [Google Scholar]
  23. NEVINS J. R. 1983; The pathway of eukaryotic mRNA formation. Annual Review of Biochemistry 52:441–466
    [Google Scholar]
  24. PETTY I. T. D., COUTTS R. H. A., BUCK K. W. 1986; Geminivirus coat protein gene promoter sequences can function in Escherichia coli. Nucleic Acids Research 14:5113
    [Google Scholar]
  25. PROUDFOOT N. J., BROWNLEE G. G. 1976; 3′ non-coding sequences in eukaryotic messenger RNA. Nature, London 263:211–214
    [Google Scholar]
  26. SANGER F., COULSON A. R. 1978; The use of thin acrylamide gels for DNA sequencing. FEBS Letters 87:107–110
    [Google Scholar]
  27. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  28. SEALEY P. G., SOUTHERN E. 1982 Gel electrophoresis of DNA. Gel Electrophoresis of Nucleic Acids: A Practical Approach39–76 Edited by Rickwood D., Hames B. D. Oxford: IRL Press;
    [Google Scholar]
  29. STANLEY J. 1983; Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature, London 305:643–645
    [Google Scholar]
  30. STANLEY J., GAY M. R. 1983; Nucleotide sequence of cassava latent virus DNA. Nature, London 301:260–262
    [Google Scholar]
  31. STEIN V. E., COUTTS R. H. A., BUCK K. W. 1983; Serological studies on tomato golden mosaic virus, a geminivirus. Journal of General Virology 64:2493–2498
    [Google Scholar]
  32. THOMAS P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
    [Google Scholar]
  33. TOWNSEND R., STANLEY J., CURSON S. J., SHORT M. N. 1985; Major polyadenylated transcripts of cassava latent virus and location of the gene encoding coat protein. EMBO Journal 4:33–37
    [Google Scholar]
  34. TULLIS R. H., RUBIN H. 1980; Calcium protects DNase I from proteinase K: a new method for the removal of contaminating RNase from DNase. Analytical Biochemistry 107:260–264
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-6-1359
Loading
/content/journal/jgv/10.1099/0022-1317-69-6-1359
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error