@article{mbs:/content/journal/jgv/10.1099/0022-1317-69-6-1331, author = "Dore, Jean-Michel and Pinck, Lothaire", title = "Plasmid DNA Containing a Copy of RNA3 Can Substitute for RNA3 in Alfalfa Mosaic Virus RNA Inocula", journal= "Journal of General Virology", year = "1988", volume = "69", number = "6", pages = "1331-1338", doi = "https://doi.org/10.1099/0022-1317-69-6-1331", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-69-6-1331", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "AlMV", keywords = "plasmid DNA", keywords = "oligonucleotide priming", abstract = "Summary The infectivity of a DNA copy of RNA3 from strain S of alfalfa mosaic virus inserted in a pT7-1 plasmid vector (clone 3pT7-1) was tested as circular DNA by co-inoculation with RNAs 1, 2 and 4 from strain B (mix I0) on young tobacco leaves. Virus multiplication was monitored by serological detection of the coat protein. Progeny RNA3 derived from circular plasmid DNA 3pT7-1 was of the same length as naturally occurring RNA3-S. Analysis by primer extension demonstrated that it had the 5′-terminal sequence of RNA3-S and not that of RNA3-B. Mix I0 also became infectious when mixed with M13 single-stranded DNA carrying RNA3-S inserted in the (-) strand orientation, probably because traces of RNA3-B present in mix I0 were protected from degradation by base-pairing and were therefore active.", }