1887

Abstract

Summary

The infectivity of a DNA copy of RNA3 from strain S of alfalfa mosaic virus inserted in a pT7-1 plasmid vector (clone 3pT7-1) was tested as circular DNA by co-inoculation with RNAs 1, 2 and 4 from strain B (mix I) on young tobacco leaves. Virus multiplication was monitored by serological detection of the coat protein. Progeny RNA3 derived from circular plasmid DNA 3pT7-1 was of the same length as naturally occurring RNA3-S. Analysis by primer extension demonstrated that it had the 5′-terminal sequence of RNA3-S and not that of RNA3-B. Mix I also became infectious when mixed with M13 single-stranded DNA carrying RNA3-S inserted in the (-) strand orientation, probably because traces of RNA3-B present in mix I were protected from degradation by base-pairing and were therefore active.

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1988-06-01
2024-04-23
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References

  1. AHLQUIST P., FRENCH R., JANDA M., LOESCH-FRIES L. S. 1984; Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proceedings of the National Academy of Sciences, U.S.A 81:7066–7070
    [Google Scholar]
  2. ANSCRGE W., LABEIT S. 1984; Field gradients improve resolution on DNA sequencing gels. Journal of Biochemical and Biophysical Methods 10:237–243
    [Google Scholar]
  3. BERNA A., GODEFROY-COLBURN T., STUSSI-GARAUD C. 1985; Preparation of an antiserum against an in vitro translation product of alfalfa mosaic virus RNA 3. Journal of General Virology 66:1669–1678
    [Google Scholar]
  4. BOSELEY P. G., TUYNS A., BIRNSTIEL M. L. 1978; Mapping of Xenopus laevis 5-8S rDNA by restriction and DNA sequencing. Nucleic Acids Research 5:1121–1137
    [Google Scholar]
  5. COLLMER C. W., KAPER J. M. 1986; Infectious transcripts from cloned cDNAs of cucumber mosaic viral satellites. Biochemical and Biophysical Research Communications 135:290–296
    [Google Scholar]
  6. CRESS D. E. M., KIEFER M. C., OWENS R. A. 1983; Construction of infectious potato spindle tuber viroid cDNA clones. Nucleic Acids Research 11:6821–6825
    [Google Scholar]
  7. DASMAHAPATRA B., DASGUPTA R., SAUNDERS K., SELLING T., GALLAGHER T., KAESBERG P. 1986; Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus. Proceedings of the National Academy of Sciences, U.S.A 83:63–66
    [Google Scholar]
  8. DAWSON W. O., BECK D. L., KNORR D. A., GRANTHAM G. L. 1986; cDNA cloning of the complete genome of tobacco virus and production of infectious transcripts. Proceedings of the National Academy of Sciences, U.S.A 83:1832–1836
    [Google Scholar]
  9. DEVOS R., VAN EMMELO J., SEURINCK-OPSOMER C, GILLIS E., FIERS W. 1976; Addition of ATP: RNA adenylyltransferase from Escherichia coli of 3′-linked oligo(A) to bacteriophage Qβ RNA and its effect on RNA replication. Biochimica et biophysica acta 447:319–327
    [Google Scholar]
  10. GERLACH W. L., BUZAYAN J. M., SCHNEIDER I. R., BRUENING G. 1986; Satellite tobacco ringspot virus RNA: biological activity of DNA clones and their in vitro transcripts. Virology 151:172–185
    [Google Scholar]
  11. GUSTAFSON G. D., MILNER J. J., MCFARLAND J. E., PEDERSEN K., LARKINS B. A., JACKSON A. O. 1982; Investigation of the complexity of barley stripe mosaic virus RNAs with recombinant DNA clones. Virology 120:182–193
    [Google Scholar]
  12. HEIDECKER G., MESSING J. 1983; Sequence analysis of zein cDNAs obtained by an efficient mRNA cloning method. Nucleic Acids Research 11:4891–4906
    [Google Scholar]
  13. JACKSON A. O., LARKINS B. A. 1976; Influence of ionic strength, pH, and chelation of divalent metals on isolation of polyribosomes from tobacco leaves. Plant Physiology 57:5–10
    [Google Scholar]
  14. JANDA M., FRENCH R., AHLQUIST P. 1987; High efficiency T7 polymerase synthesis of infectious RNA from cloned brome mosaic virus cDNA and effects of 5′ extensions on transcript infectivity. Virology 158:259–262
    [Google Scholar]
  15. JASPARS E. M. J. 1985 Interaction of alfalfa mosaic virus nucleic acid and protein. Molecular Plant Virology 1156–221 Edited by Davies J. W. Boca Raton: CRC Press;
    [Google Scholar]
  16. KAPLAN K. M., LUBINSKI J., DASGUPTA A., RACANIELLO V. R. 1985; In vitro synthesis of infectious poliovirus RNA. Proceedings of the National Academy of Sciences, U.S.A 82:8424–8428
    [Google Scholar]
  17. LANGEREIS K., NEELEMAN L., BOL J. F. 1986a; Biologically active transcripts of cloned DNA of the coat protein messenger of two plant viruses. Plant Molecular Biology 6:281–288
    [Google Scholar]
  18. LANGEREIS K., MUGNIER M. A., CORNELISSEN B. J. C, PINCK L., BOL J. F. 1986b; Variable repeats and poly(A)-stretches in the leader sequence of alfalfa mosaic virus RNA3. Virology 154:409–414
    [Google Scholar]
  19. LOESCH-FRIES S., JARVIS N. P., KRAHN K. J., NELSON S. E., HALL T. C. 1985; Expression of alfalfa mosaic virus RNA4 cDNA transcripts in vitro and in vivo. Virology 146:177–187
    [Google Scholar]
  20. MELTON D. A., KRIEG P. A., REBAGLIATI M. R., MANIATIS T., ZINN K., GREEN M. R. 1984; Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Research 12:7035–7056
    [Google Scholar]
  21. MESHI T., ISHIKAWA M., OHNO T., OKADA Y., SANO T., UEDA I., SHIKATA E. 1984; Double-stranded cDNAs of hop stunt viroid are infectious. Journal of Biochemistry 95:1521–1524
    [Google Scholar]
  22. MESHI T., ISHIKAWA M., MOTOYOSHI F., SEMBA K., OKADA Y. 1986; In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus. Proceedings of the National Academy of Sciences, U.S.A 83:5043–5047
    [Google Scholar]
  23. MIZUTANI S., COLONNO R. J. 1985; In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14. Journal of Virology 56:628–632
    [Google Scholar]
  24. PATERSON B. M., ROBERTS B. E., KUFF E. L. 1977; Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation. Proceedings of the National Academy of Sciences, U.S.A 74:4370–4374
    [Google Scholar]
  25. PINCK L., FAUQUET C. 1975; Analysis of pancreatic ribonuclease digestion products of alfalfa mosaic virus ribonucleic acids. European Journal of Biochemistry 57:441–451
    [Google Scholar]
  26. QU L. H., MICHOT B., BACHELLERIE J. P. 1983; Improved methods for structure probing in large RNAs: a rapid ‘heterologous’ sequencing approach is coupled to direct mapping of nuclease accessible sites. Nucleic Acids Research 11:5903–5920
    [Google Scholar]
  27. RACANIELLO V. R., BALTIMORE D. 1981; Cloned poliovirus complementary DNA is infectious in mammalian cells. Science 214:916–919
    [Google Scholar]
  28. RAVELONANDRO M., GODEFROY-COLBURN T., PINCK L. 1983; Structure of the 5′-terminal region of the genomic RNAs from two strains of alfalfa mosaic virus. Nucleic Acids Research 11:2815–2826
    [Google Scholar]
  29. VAN EMMELO J., AMELOOT P., FIERS W. 1987; Expression in plants of the cloned satellite tobacco necrosis virus genome and of derived insertion mutants. Virology 157:480–487
    [Google Scholar]
  30. VISVADER J. E., FORSTER A. C., SYMONS R. H. 1985; Infectivity and in vitro mutagenesis of monomeric cDNA clones of citrus exocortis viroid indicates the site of processing of viroid precursors. Nucleic Acids Research 13:5843–5856
    [Google Scholar]
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