1887

Abstract

Summary

Single-stranded circular DNA associated with foliar decay disease of coconut palm in Vanuatu (FDD-DNA) has been purified and three fragments have been cloned in plasmid pUC19. Clones labelled with P by nick translation were used as specific probes for FDD-DNA in dot blot and Southern transfer hybridization assays. These assays were more sensitive than the polyacrylamide gel electrophoresis assay developed previously for diagnosis of FDD. Hybridization tests showed that FDD-DNA had no detectable sequence similarity to the geminivirus infecting which grows alongside FDD-infected coconut palms; that , which is the host plant of the vector of FDD, , is not infected with FDD-DNA; and that the symptomless coconut variety, Vanuatu Tall, is susceptible to infection with FDD-DNA. Native FDD-DNA migrates as two distinct bands in polyacrylamide gels, and both hybridize to the probe. Previous estimates and data presented here show that FDD-DNA is approximately half the size of the geminivirus DNA (approx. 2350 nucleotides) and support the view that FDD is caused by a virus not typical of any plant virus taxonomic group.

Keyword(s): coconut palm , FDD-DNA and geminivirus
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/content/journal/jgv/10.1099/0022-1317-69-6-1323
1988-06-01
2024-03-29
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References

  1. CALVEZ C, RENARD J. L., MARTY G. 1980; Tolerance of the hybrid coconut Local × Rennell to New Hebrides disease. Oléagineux 35:443–451
    [Google Scholar]
  2. CHU P. W. G., HELMS K. 1987; Purification and characterization of a novel single-stranded DNA virus associated with subterranean clover stunt virus in Australia. Proceedings, 7th International Congress of Virology233
    [Google Scholar]
  3. DOLLET M., ACCOTTO G. P., LISA V., MENISSIER J., BOCCARDO G. 1986; A geminivirus, serologically related to maize streak virus, from Digitaria sanguinalis from Vanuatu. Journal of General Virology 67:933–937
    [Google Scholar]
  4. DONSON J., ACCOTTO G. P., BOULTON M. I., GUNN H., MARKHAM P. G., MULLINEUX P. M., WOOLSTON C., DAVIES J. W. 1987; The organization and expression of a geminivirus serologically related to maize streak virus, from Digitaria sanguinalis. Proceedings, 7th International Congress of Virology234
    [Google Scholar]
  5. FRANCKI R. I. B., HATTA T. 1980; Chloris striate mosaic virus. CMI/AAB Descriptions of Plant Viruses 221:
    [Google Scholar]
  6. HARRISON B. D. 1985; Advances in geminivirus research. Annual Review of Phytopathology 23:55–82
    [Google Scholar]
  7. JULIA J. F. 1982; Myndus taffini (Homoptera Cixiidae), vector of foliar decay of coconuts in Vanuatu. Oléagineux 37:409–414
    [Google Scholar]
  8. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. MAXAM A. M., GILBERT W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  10. RANDLES J. W., JULIA J. F., CALVEZ C., DOLLET M. 1986; Association of single-stranded DNA with the foliar decay disease of coconut palm in Vanuatu. Phytopathology 76:889–894
    [Google Scholar]
  11. RANDLES J. W., HANOLD D., JULIA J. F. 1987; Small circular single-stranded DNA associated with foliar decay disease of coconut palm in Vanuatu. Journal of General Virology 68:273–280
    [Google Scholar]
  12. SOUTHERN E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  13. TISCHER J., GELDERBLOM H., VETTERMAN W., KOCH M. A. 1982; A very small porcine virus with circular single-stranded DNA. Nature, London 295:64–66
    [Google Scholar]
  14. VIEIRA J., MESSING J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
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