1887

Journal of General Virology header logo

Volume 69, Issue 6

Genome Homology and Host Range of Some SPβ-related Bacteriophages of and Free

Abstract

Summary

Temperate bacteriophages SBβ, ø3T and SPR and phage H2 were compared with respect to DNA-DNA homology by Southern blot analysis to each other and to members of the genus . The results show that H2 is a distantly related member of the group III phages. Detectable homology to group III phages could be found in the DNA of several other species, including and , demonstrating the widespread occurrence of this group of phages. The host ranges for the phages SPβ, SPR and H2 were determined by adsorption efficiency and by the ability of erythromycin resistance- and chloramphenicol resistance-transducing phages to convert susceptible host strains. Of the three phages examined, only H2 was capable of infecting . Based on these results we propose that group III phages should be divided into three subgroups: SPβ, ø3T, Rho11, IG1, IG3 and Z (subgroup 1), SPR (subgroup 2) and H2 (subgroup 3).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus sp. , genome homology and phage
© Society for General Microbiology 1988
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-6-1307
1988-06-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/6/JV0690061307.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-6-1307&mimeType=html&fmt=ahah

References

  1. BEHRENS B., NOYER-WEIDNER M., PAWLEK B., LAUSTER R., BALGANESH T. S., TRAUTNER T. A. 1987; Organization of multispecific DNA methyltransferases encoded by temperate Bacillus subtilis phages. EMBO Journal 6:1137–1142
     [Google Scholar]
  2. CHIN T., BURGER M. M., GLASER L. 1966; Synthesis of teichoic acids. VI. The formation of multiple wall polymers in Bacillus subtilis W23. Archives of Biochemistry and Biophysics 116:358–367
     [Google Scholar]
  3. DEAN D. H., FORT C. L., HOCH J. A. 1978; Characterization of temperate phages of Bacillus subtilis. Current Microbiology 1:213–217
     [Google Scholar]
  4. ESTRELA A. I., LENCASTRE H., ARCHER L. J. 1986; Resistance of a Bacillus subtilis mutant to a group of temperate phages. Journal of General Microbiology 132:411–415
     [Google Scholar]
  5. FERNANDES R., LENCASTRE H., ARCHER L. 1986; Three new temperate phages of Bacillus subtilis. Journal of General Microbiology 132:661–668
     [Google Scholar]
  6. FINK P. S., ZAHLER S. A. 1982; Specialized transduction of the ilvD-thyB-ilvA region mediated by Bacillus subtilis bacteriophage SPβ. Journal of Bacteriology 150:1274–1279
     [Google Scholar]
  7. FINK P. S., KORMAN R. Z., ODEBRALSKI I. M., ZAHLER S. A. 1981; Bacillus subtilis phage SPβcl is a deletion mutant of SPβ. Molecular and General Genetics 182:514–515
     [Google Scholar]
  8. FUCHS R., BLAKESLEY R. 1983; Guide to the use of type II restriction endonucleases. Methods in Enzymologv 100:3–38
     [Google Scholar]
  9. GLASER L., IONESCO H., SCHAEFFER P. 1966; Teichoic acids as components of a specific phage receptor in Bacillus subtilis. Biochimica et biophysica acta 124:415–417
     [Google Scholar]
  10. GAGEÜNTHERT U., RELNERS L. 1987; Bacillus subtilis phage SPR codes for a DNA methyltransferase with triple sequence specificity. Nucleic Acids Research 15:3689–3702
     [Google Scholar]
  11. GÜNTHERT U., TRAUTNER T. A. 1984; DNA methyltransferases of Bacillus subtilis and its phages. Current Topics in Microbiology and Immunology 108:11–22
     [Google Scholar]
  12. GÜNTHERT U., LAUSTER R., REINERS L. 1986; Multispecific DNA methyltransferases from Bacillus subtilis phages. European Journal of Biochemistry 159:485–492
     [Google Scholar]
  13. HANAHAN D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
     [Google Scholar]
  14. HEMPHILL H. E., GAGE L, ZAHLER S. A., KORMAN R. Z. 1980; Prophage-mediated production of a bacteriocin-like substance by SP/Î lysogens of Bacillus subtilis. Canadian Journal of Microbiology 26:1328–1333
     [Google Scholar]
  15. HOLMES D. S., QUIGLEY M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
     [Google Scholar]
  16. HORINOUCHI S., WEISBLUM B. 1982; Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. Journal of Bacteriology 150:815–825
     [Google Scholar]
  17. LIPSKY R. H., ROSENTHAL R., ZAHLER S. A. 1981; Defective specialized SPβ transducing phages of Bacillus subtilis that carry the sup-3 or sup-44 gene. Journal of Bacteriology 148:1012–1015
     [Google Scholar]
  18. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  19. MACKEY C. J., ZAHLER S. A. 1982; Insertion of phage SPβ into the citF gene of Bacillus subtilis, and specialized transduction of the ilvBC-leu genes. Journal of Bacteriology 151:1222–1229
     [Google Scholar]
  20. MCLAUGHLIN J. R., WONG H. C, TING Y. E., VAN ARSDELL J. N., CHANG S. 1986; Control of lysogeny and immunity of Bacillus subtilis temperate bacteriophage SPβ by its d gene. Journal of Bacteriology 167:952–959
     [Google Scholar]
  21. NOYER-WEIDNER M., JENTSCH S., KUPSCH J., BERGBAUER M., TRAUTNER T. 1985; DNA methyltransferase genes of Bacillus subtilis phages: structural relatedness and gene expresson. Gene 35:143–150
     [Google Scholar]
  22. ODEBRALSKI J. M., ZAHLER S. A. 1982; Specialized transduction of the kauA and citK genes of Bacillus subtilis by phage ϕ3T. Abstracts of the American Society for Microbiology130
     [Google Scholar]
  23. ROSENTHAL R., TOYE P. A., KORMAN R. Z., ZAHLER S. A. 1979; The prophage of SPβc2drirÄ:-l, a defective specialized transducing phage of Bacillus subtilis. Genetics 92:721–739
     [Google Scholar]
  24. RUTBERG L. 1982 Temperate phages of Bacillus subtilis. Molecular Biology of the Bacilli246–268 Edited by Dubnau D. New York & London: Academic Press;
     [Google Scholar]
  25. SARGENT M. G., DAVIES S., BENNETT M. 1985; Potentiation of a nucleolytic activity in Bacillus subtilis. Journal of General Microbiology 131:2795–2804
     [Google Scholar]
  26. SPANCAKE G. A., DAIGNAULT S. D., HEMPHILL H. E. 1987; Genome homology and divergence in the SPβ-related phages of Bacillus subtilis. Canadian Journal of Microbiology 33:249–255
     [Google Scholar]
  27. STROYNOWSKI I. T. 1978 Molecular biology of thymidylate synthetase genes in Bacillus subtilis and its phage <j>3T Ph.D. thesis, Stanford University
    [Google Scholar]
  28. STROYNOWSKI I. T. 1981; Distribution of phage ϕ3T homologous deoxyribonucleic sequences in Bacillus subtilis 168, related phages, and other Bacillus species. Journal of Bacteriology 148:91–100
     [Google Scholar]
  29. TUCKER R. G. 1969; Acquisition of a thymidylate synthetase activity by a thymine-requiring mutant of Bacillus subtilis following infection by the temperate phage </>3. Journal of General Virology 4:489–504
     [Google Scholar]
  30. WARNER F. D., KITOS G. A., ROMANO M. P., HEMPHILL H. E. 1977; Characterization of SPβ: a temperate phage from Bacillus subtilis 168M. Canadian Journal of Microbiology 23:45–51
     [Google Scholar]
  31. WEINER M. P. 1986 Characterization of phage H2 Ph.D. thesis, Cornell University
    [Google Scholar]
  32. YAMAMOTO K. R., ALBERTS B. M., BENZINGER R., LAWTHORNE L., TREIBER G. 1970; Rapid phage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
     [Google Scholar]
  33. YASBIN R. E., WILSON G. A., YOUNG F. E. 1975; Transformation and transfection in lysogenic strains of Bacillus subtilis: evidence for selective induction of prophage in competent cells. Journal of Bacteriology 121:296–304
     [Google Scholar]
  34. YASBIN R. E., MAINO V. C., YOUNG F. E. 1976; Bacteriophage resistance in Bacillus subtilis 168, W23 and interstrain transformants. Journal of Bacteriology 125:1120–1126
     [Google Scholar]
  35. YOUNGMAN P., ZUBER P., PERKINS J. B., SANDMAN K., IGO M., LOSICK R. 1985; New ways to study developmental genes in spore-forming bacteria. Science 228:285–291
     [Google Scholar]
  36. ZAHLER S. A. 1988 Temperate bacteriophages of Bacillus subtilis. The Viruses 8 The Bacteriophages 559–592 Edited by Calendar R. New York & London: Plenum Press;
     [Google Scholar]
  37. ZAHLER S. A., KORMAN R. Z., ROSENTHAL R., HEMPHILL H. E. 1977; Bacillus subtilis phage SPβ: localization of the prophage attachment site, and specialized transduction. Journal of Bacteriology 129:556–558
     [Google Scholar]
  38. ZAHLER S. A., KORMAN R. Z., ODEBRALSKI J. M., FINK P. S., MACKEY C. J., POUTRE C. G., LIPSKY R. H., YOUNGMAN P. S. 1982 Genetic manipulations with phage SPβ. Molecular Cloning and Gene Regulation in Bacillus41–50 Edited by Hoch J. A., Chang S., Ganesan A. T. New York & London: Academic Press;
     [Google Scholar]
  39. ZAHLER S. A., KORMAN R. Z., THOMAS C., ODEBRALSKI J. M. 1987a; Temperate bacteriophages of Bacillus amyloliquefaciens. Journal of General Microbiology 133:2933–2935
     [Google Scholar]
  40. ZAHLER S. A., KORMAN R. Z., THOMAS C, FINK P. S., WEINER M. P., ODEBRALSKI J. M. 1987b; H2, a temperate bacteriophage isolated from Bacillus amyloliquefaciens strain H. Journal of General Microbiology 133:2937–2944
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-6-1307
Loading
Genome Homology and Host Range of Some SPβ-related Bacteriophages of Bacillus Subtilis and Bacillus amyloliquefaciens
J. Gen. Virol. 69, 1307 (1988); https://doi.org/10.1099/0022-1317-69-6-1307
/content/journal/jgv/10.1099/0022-1317-69-6-1307
/content/journal/jgv/10.1099/0022-1317-69-6-1307
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error