1887

Abstract

Summary

A temperature-sensitive mutant of herpes simplex virus type 1, Q26, was shown to contain an amino acid substitution in glycoprotein H (gH). The mutant entered cells efficiently at the non-permissive temperature and replicated to give nearly normal yields of intracellular infectivity. The intracellular virions contained, predominantly, an immature form of gH and no gH was found on the surface of infected cells. Excreted virions were devoid of gH and were not infectious. Virions excreted at the permissive temperature were infectious and contained gH and no loss of gH resulted from incubation of these virions at the non-permissive temperature. The temperature-sensitive phenotype apparently results from the loss of gH from virions during their transport to the cell surface, and since loss of gH is accompanied by loss of infectivity we conclude that gH is an essential component of the infectious virion.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-6-1147
1988-06-01
2022-10-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/6/JV0690061147.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-6-1147&mimeType=html&fmt=ahah

References

  1. BANKIER A. T., BARRELL B. G. 1983 Shotgun DNA sequencing. Techniques in the Life Sciences B5081–34 Edited by Flavell R. A. Amsterdam: Elsevier;
    [Google Scholar]
  2. BUCKMASTER E. A., GOMPELS U., MINSON A. C. 1984; Characterization and physical mapping of an HSV-1 glycoprotein of approximately 115 × 103 molecular weight. Virology 139:408–413
    [Google Scholar]
  3. CAI W., PERSON S., WARNER S. C, ZHOU J., DELUCA N. 1987; Linker-insertion nonsense and restriction site-deletion mutations of the gB glycoprotein gene of herpes simplex virus type 1. Journal of Virology 61:714–721
    [Google Scholar]
  4. CAMPADELLI-FIUME G., POLETTI L., DALL’OLIO F., SERAFINI-CESSI F. 1982; Infectivity and glycoprotein processing of herpes simplex virus type 1 grown in a ricin-resistant cell line deficient in N-acetylglucosaminetransferase I. Journal of Virology 43:1061–1071
    [Google Scholar]
  5. CHU C-T., PARRIS D. S., DIXON R. A. F., FARBER F. E., SCHAFFER P. A. 1979; Hydroxylamine mutagenesis of HSV DNA and DNA fragments: introduction of mutations into selected regions of the viral genome. Virology 98:168–181
    [Google Scholar]
  6. GOMPELS U., MINSON A. C. 1986; The properties and sequence of glycoprotein H of herpes simplex virus type 1. Virology 153:230–247
    [Google Scholar]
  7. HEINE J. W., HONESS R. W., CASSAI E., ROIZMAN B. 1974; The proteins specified by herpes simplex virus. XII. The virion polypeptides specified by type 1 strains. Journal of Virology 14:640–651
    [Google Scholar]
  8. HOLLAND T. C, HOMA F. C, MARLIN S. D., LEVINE M., GLORIOSO J. 1984; Herpes simplex virus type 1 glycoprotein C-negative mutants exhibit multiple phenotypes including secretion of truncated glycoproteins. Journal of Virology 52:566–574
    [Google Scholar]
  9. JOHNSON D. C, SPEAR P. G. 1982; Monensin inhibits the processing of herpes simplex virus glycoproteins, their transport to the cell surface and the egress of virions from infected cells. Journal of Virology 43:1102–1112
    [Google Scholar]
  10. KELLER P. M., DAVISON A. J., LOWE R. S., RIEMEN M. W., ELLIS R. W. 1987; Identification and sequence of the gene encoding gpIII, a major glycoprotein of varicella-zoster virus. Virology 157:526–533
    [Google Scholar]
  11. LITTLE S. P., JOFFRE J. T., COURTNEY R. J., SCHAFFER P. A. 1981; A virion associated glycoprotein essential for infectivity of herpes simplex virus type 1. Virology 115:149–160
    [Google Scholar]
  12. LONGNECKER R., ROIZMAN B. 1986; Generation of an inverting HSV-1 mutant lacking the L-S junction a sequences, an origin of DNA synthesis, and several genes including those specifying glycoprotein E and the a47 gene. Journal of Virology 58:583–591
    [Google Scholar]
  13. LONGNECKER R., ROIZMAN B. 1987; Clustering of genes dispensable for growth in culture in the S-component of the HSV-1 genome. Science 236:573–576
    [Google Scholar]
  14. LONGNECKER R., CHATTERJEE S., WHTTLEY R. J., ROIZMAN B. 1987; Identification of a HSV-1 glycoprotein gene within a gene cluster dispensable for growth in cell culture. Proceedings of the National Academy of Sciences, U.S.A 84:4303–4307
    [Google Scholar]
  15. MCGEOCH D. J., DAVISON A. J. 1986; DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein H and identification of homologues in the genomes of varicella-zoster virus and Epstein-Barr virus. Nucleic Acids Research 14:4281–4292
    [Google Scholar]
  16. MCLEAN C, BUCKMASTER A., HANCOCK D., BUCHAN A., FULLER A., MINSON A. C. 1982; Monoclonal antibodies to three non-glycosylated antigens of herpes simplex virus type 2. Journal of General Virology 63:297–305
    [Google Scholar]
  17. MESSING J., VIEIRA J. 1982; A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276
    [Google Scholar]
  18. METSIKKO K., SIMONS K. 1986; The budding mechanism of spikeless vesicular stomatitis virus particles. EMBO Journal 5:1913–1920
    [Google Scholar]
  19. MINSON A. C, HODGMAN T. C, DIGARD P., HANCOCK D. C, BELL S. E., BUCKMASTER E. A. 1986; An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization. Journal of General Virology 67:1001–1013
    [Google Scholar]
  20. NOBLE A. G., LEE G. T. Y., SPRAGUE R., PARISH M. L., SPEAR P. G. 1983; Anti-gD monoclonal antibodies inhibit cell fusion induced by herpes simplex virus type I. Virology 129:218–244
    [Google Scholar]
  21. RICHMAN D. D., BUCKMASTER A., BELL S., HODGMAN C., MINSON A. C. 1986; Identification of a new glycoprotein of herpes simplex virus type 1 and genetic mapping of the gene that codes for it. Journal of Virology 57:647–655
    [Google Scholar]
  22. RIGBY P. W. J., DIECKMANN M., RHODES C., BERG P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  23. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  24. SHOWALTER S. D., ZWEIG M., HAMPAR B. 1981; Monoclonal antibodies to herpes simplex virus type 1 proteins including the immediate early protein ICP4. Infection & Immunity 34:684–692
    [Google Scholar]
  25. SIMINOFF P., MENEFEE M. G. 1966; Normal and 5-bromo-deoxyuridine-inhibited development of herpes simplex virus. An electron microscope study. Experimental Cell Research 44:241–255
    [Google Scholar]
  26. SOUTHERN E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  27. SPEAR P. G. 1984 Glycoproteins specified by herpes simplex viruses. The Herpesviruses 3315–356 Edited by Roizman B. New York & London: Plenum Press;
    [Google Scholar]
  28. SPINDLER K. R., ROSSER D. S. E., BERK A. J. 1984; Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in E. coli. Journal of Virology 49:132–141
    [Google Scholar]
  29. WEBER P. C, LEVINE M., GLORIOSO I. C. 1987; Rapid identification of non-essential genes of herpes simplex virus type 1 by Tn5 mutagenesis. Science 236:576–579
    [Google Scholar]
  30. WELLER S. K., ASCHMAN D. P., SACKS W. R., COEN D. M., SCHAFFER P. A. 1983; Genetic analysis of temperature sensitive mutants of HSV-1: the combined use of complementation and physical mapping for cistron assignment. Virology 130:290–305
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-6-1147
Loading
/content/journal/jgv/10.1099/0022-1317-69-6-1147
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error