1887

Abstract

Summary

Complexes of substructural elements of bacteriophage T4 (baseplates, baseplate-core complexes) with long tail fibres were obtained for the first time by complementation . A study of the organization of the complexes was carried out by PAGE, electron microscopy and sedimentation analysis. About 90% of baseplates and baseplate-core complexes were combined with fibres. However, the number of the attached fibres varied from one to six. On the basis of the data obtained, we proposed that the attachment of long tail fibres can occur before the assembly of the whole bacteriophage.

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1988-05-01
2021-10-17
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References

  1. BARAN G. J., BLOOMFIELD V. A. 1978; Tail fibre attachment in bacteriophage T4D studied by quasi-elastic light scattering-band electrophoresis. Biopolymers 17:2015–2028
    [Google Scholar]
  2. BRENNER S., HORNE R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochimica et biophysica acta 34:103–110
    [Google Scholar]
  3. CONLEY M. P., WOOD W. B. 1975; Bacteriophage T4 whiskers: a rudimentary environment-sensing device. Proceedings of the National Academy of Sciences, U.S.A 72:3701–3705
    [Google Scholar]
  4. CRAWFORD J. T., GOLDBERG E. B. 1980; The function of tail fibres in triggering baseplate expansion of bacteriophage T4. Journal of Molecular Biology 139:679–690
    [Google Scholar]
  5. DICKSON R. C. 1973; Assembly of bacteriophage T4 tail fibres. IV. Subunit composition of tail fibres and fibre precursors. Journal of Molecular Biology 79:633–647
    [Google Scholar]
  6. DUDA R. L., EISERLING F. A. 1982; Evidence for an internal component of the bacteriophage T4D tail core: a possible length-determining template. Journal of Virology 43:714–720
    [Google Scholar]
  7. EDGAR R. S., LIELAUSIS I. 1968; Some steps in the assembly of bacteriophage T4. Journal of Molecular Biology 32:263–271
    [Google Scholar]
  8. KELLENBERGER E., BOLLE A., BOY DE LA TOUR E., EPSTEIN R. H., FRANKLIN N. C, JERNE N. K., REALE-SCAFATI A., SECHAUD J., BENDET I., GOLDSTEIN D. 1965; Functions and properties related to the tail fibres of bacteriophage T4. Virology 26:419–440
    [Google Scholar]
  9. KING J. 1971; Bacteriophage T4 tail assembly: four steps in core formation. Journal of Molecular Biology 58:693–709
    [Google Scholar]
  10. NASHIMOTO H., UCHIDA H. 1969; Indole as an activator for in vitro attachment of tail fibres in the assembly of bacteriophage T4D. Virology 37:1–7
    [Google Scholar]
  11. SELIVANOV N. A., GOLICHINA N. L., RUSTEMBEKOV O. S., MESYANZHINOV V. V. 1981; Regulation of assembly and attachment of the long fibres of bacteriophage T4. Doklady Akademii nauk SSSR 260:1262–1266 In Russian
    [Google Scholar]
  12. SHCHERBUKHIN V. V., GUERMANT C. 1975; Anomalous sedimentation behaviour of the E. coli ribosomal system. Biophysical Chemistry 3:21–34
    [Google Scholar]
  13. TERZAGHL E., TSUGITA A. 1966; In vitro assembly of T4 phage. Japanese Journal of Genetics 41:484–490
    [Google Scholar]
  14. WAGENKNECHT T., BLOOMFIELD V. A. 1977; In vitro polymerization of bacteriophage T4D tail core subunits. Journal of Molecular Biology 116:347–359
    [Google Scholar]
  15. WOOD W. B., CONLEY M. P. 1979; Attachment of tail fibres in bacteriophage T4 assembly: role of the phage whiskers. Journal of Molecular Biology 127:15–29
    [Google Scholar]
  16. WOOD W. B., HENNINGER E. 1969; Attachment of tail fibres in bacteriophage T4 assembly: some properties of the reaction in vitro and its genetic control. Journal of Molecular Biology 39:603–618
    [Google Scholar]
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