%0 Journal Article %A Yoon, Ji-Won %A Ko, William %A Bae, Yong-Soo %A Pak, Chin Y. %A Amano, Kazuhiko %A Eun, Hyone-Myong %A Kim, Myung K. %T Identification of Antigenic Differences Between the Diabetogenic and Non-diabetogenic Variants of Encephalomyocarditis Virus Using Monoclonal Antibodies %D 1988 %J Journal of General Virology, %V 69 %N 5 %P 1085-1090 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-69-5-1085 %K antigenic differences %K EMC virus %K cardiovirus %I Microbiology Society, %X Summary The M variant of encephalomyocarditis (EMC) virus consists of two biologically distinct variants: one, diabetogenic D variant (EMC-D) and the other, non-diabetogenic B variant (EMC-B). These two variants cannot be distinguished by hyperimmune sera. Monoclonal antibodies were generated against EMC-D or EMC-B to identify antigenic differences between these two variants. Fourteen independent hybrid cell lines, selected from seven separate fusions of mouse myeloma cells to spleen cells isolated from mice immunized with EMC-D, consisted of 12 hybrids which produced monoclonal antibodies that neutralized both EMC-D and EMC-B, and two hybrids (ED-HJ-23 and ED-HJ-31) which produced monoclonal antibodies that neutralized EMC-D but not EMC-B. Similarly, 16 independent hybrid cell lines, selected from eight separate fusions using spleen cells prepared from mice immunized with EMC-B, consisted of 15 hybrids which produced monoclonal antibodies neutralizing both EMC-D and EMC-B, and one hybrid (EB-48A-F1) which produced antibody that neutralized EMC-B, but not EMC-D. The specificities of these monoclonal antibodies (ED-HJ-23, ED-HJ-31, EB-48A-F1) were further confirmed using an immunofluorescent technique. The D variant-specific monoclonal antibodies reacted with cells infected with EMC-D but not EMC-B. In contrast, the B variant-specific monoclonal antibody reacted with the cells infected with EMC-B but not EMC-D. It is concluded that the EMC-D- and EMC-B-specific monoclonal antibodies are able to identify antigenic differences between diabetogenic and non-diabetogenic variants of EMC virus which cannot be distinguished by hyperimmune sera. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-69-5-1085