1887

Abstract

Summary

5-Azacytidine (5-AZC) reduces cytosine methylation in DNA and has been reported to activate quiescent virus genes. Treatment of explant cultures of latently herpes simplex virus type 2 (HSV-2)-infected guinea-pig dorsal root ganglia and spinal cords with 5-AZC significantly enhanced the rate of HSV recovery. Both the number of isolates from ganglia ( < 0.001) and the rate of recovery ( < 0.001) were significantly increased with the addition of 50 µ-5-AZC to explant cultures. Increased virus recovery appeared to be due to the induction of reactivation of latent virus, rather than an increase in replication, since 5-AZC inhibited HSV replication. These data support a role for methylation in HSV latency and reactivation.

Keyword(s): 5-azacytidine , HSV-2 , latency and methylation
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-5-1079
1988-05-01
2019-10-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-5-1079
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error