1887

Abstract

Summary

A bacterial expression vector was constructed to encode a fusion protein which had, at its carboxy terminus, a polypeptide encoded within the 5′ proximal open reading frame of the coronavirus MHV-JHM mRNA 4. This polypeptide was isolated and used to produce an antiserum. The antiserum reacted specifically with a 15000 polypeptide synthesized in MHV-JHM-infected cells, or translations of infected cell poly(A) RNA enriched for mRNA 4. These results demonstrate the translational activity of mRNA 4 during infection, identify conclusively the translation product and provide a means to investigate the synthesis and function of this protein.

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/content/journal/jgv/10.1099/0022-1317-69-5-1041
1988-05-01
2019-10-21
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-5-1041
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