The nucleotide sequence of an 11·2 kilobase fragment of the fowlpox virus genome is presented. The fragment comes from near one end of the genome and contains part of the terminal inverted repeat. Twenty open reading frames (ORFs) are predicted from the sequence and are classified into 13 major and seven minor ORFs. The 100 base pairs immediately upstream of each ORF are up to 83% AT-rich, with some motifs similar to those seen in vaccinia virus early gene promoters. The TTTTTNT element which has been identified as a termination signal for vaccinia virus early genes is also found downstream of several ORFs. Three ORFs are predicted to specify polypeptides with significant homology to proteins coded by genes near termini of orthopoxvirus genomes: the vaccinia virus 42K early gene and 32·5K host range gene, and the cowpox virus 38K red pock gene. In addition, there are two families of ORFs within the fragment which potentially encode related polypeptides. One of these, family B, contains three ORFs which are related to those of chicken and rat hepatic lectins.
ARCHARDL. C, MACKETTM., BARNESD. E., DUMBELLK. R.1984; The genome structure of cowpox virus white pock variants. Journal of General Virology 65:875–886
BANKIERA. T., BARRELLB. G.1983 Shotgun DNA sequencing. Techniques in the Life Sciences (BiochemistryB5Techniques in Nucleic Acid Biochemistry1–34 Edited by FlavellR. A. Amsterdam: Elsevier;
BIGGINM. D., GIBSONT. J., HONGG. F.1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A 80:3963–3965
BINNSM. M., STENZLERL., TOMLEYF. M., CAMPBELLI., BOURSNELLM. E. G.1987; Identification by a random sequencing strategy of the fowlpoxvirus DNA polymerase gene, its nucleotide sequence and comparison with other viral DNA polymerases. Nucleic Acids Research 15:6563–6573
DRICKAMERK.1981; Complete amino acid sequence of a membrane receptor for glycoproteins. Sequence of the chicken hepatic lectin. Journal of Biological Chemistry 256:5827–5839
DRILLIENR., SPEHNERD., VILLEVALD., LECOCQJ. P.1987; Similar genetic organization between a region of fowlpoxvirus DNA and the vaccinia virus HindIII J fragment despite divergent location of the thymidine kinase gene. Virology 160:203–209
DUMBELLK. R., ARCHARDL. C.1980; Comparison of white pock (h) mutants of monkeypox virus with parental monkeypox and with variola-like viruses isolated from animals. Nature, London 286:29–32
GILLARDS., SPEHNERD., DRILLIENR., KIRNA.1986; Localization and sequence of a vaccinia virus gene required for multiplication in human cells. Proceedings of the National Academy of Sciences, U.S.A 83:5573–5577
HOLLANDE. C, LEUNGJ. O., DRICKAMERK.1984; Rat liver asialoglycoprotein receptor lacks a cleavable NH2-terminal signal sequence. Proceedings of the National Academy of Sciences, U.S.A 81:7338–7342
MACKETTM., SMITHG. L., MOSSB.1982; Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proceedings of the National Academy of Sciences, U.S.A 79:7415–7419
MOCKETTA. P. A., SOUTHEED., TOMLEYF. M., DEUTERA.1987; Fowlpoxvirus: its structural proteins and immunogens and the detection of viral-specific antibodies by ELISA. Avian Pathology 16:493–504
MOYERR. W., GRAVESR. L., ROTHEC. T.1980; The white pock (u) mutants of rabbit poxvirus. III. Terminal DNA sequence duplication and transposition in rabbit poxvirus. Cell 22:545–553
MüLLERH. K., WITTEKR., SCHAFFNERW., SCHÜMPERLID., MENNAA., WYLERR.1978; Comparison of five poxvirus genomes by analysis with restriction endonucleases HindIII, Bami and EcoRI. Journal of General Virology 38:135–147
PANICALID., PAOLETTIE.1982; Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proceedings of the National Academy of Sciences, U.S.A 79:4927–4931
PICKUPD. I., BASTIAD., STONEH. O., JOKLIKW. K.1984; Sequence of terminal regions of cowpox virus DNA: arrangement of repeated and unique sequence elements. Proceedings of the National Academy of Sciences, U.S.A 81:7112–7116
PICKUPD. J., INKB. S., HUW., RAYC. A., JOKLIKW. K.1986; Hemorrhage in lesion caused by cowpox virus is induced by a viral protein that is related to plasma protein inhibitors of serine proteases. Proceedings of the National Academy of Sciences, U.S.A 83:7698–7702
PLUCIENNICZAKA., SCHROEDERE, ZETTLEMEISSLG., STREEKR. E.1985; Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome. Nucleic Acids Research 13:985–998
ROHRMANNG., YUENL., MOSSB.1986; Transcription of vaccinia virus early genes by enzymes isolated from vaccinia virions terminates downstream of a regulatory sequence. Cell 46:1029–1035
ROSELJ. L., EARLP. L., WEIRJ. P., MOSSB.1986; Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment. Journal of Virology 60:436–449
SANGERF., NICKLENS., COULSONA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
STADENR.1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
STADENR.1984c; Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes. Nucleic Acids Research 12:551–567
UPTONC., MCFADDENG.1986; Tumorigenic poxviruses: analysis of viral DNA sequences inplicated in the tumorigenicity of Shope fibroma virus and malignant rabbit virus. Virology 152:308–321
UPTONC, MACENJ. L., MCFADDENG.1987; Mapping and sequencing of a gene from myxoma virus that is related to those encoding epidermal growth factor and transforming growth factor. Journal of Virology 61:1271–1275
VENKATESANS., GERSHOWITZA., MOSSB.1982; Complete nucleotide sequence of two adjacent early vaccinia virus genes located within the inverted terminal repetition. Journal of Virology 44:637–646
YUENL., MOSSB.1986; Multiple 3′ ends of mRNA encoding vaccinia virus growth factor occur within a series of repeated sequences downstream of T clusters. Journal of Virology 60:320–323