Infection of Vero cells with Tacaribe virus stocks containing a high ratio of standard (plaque-forming) viruses to defective interfering particles (DIP) induced inhibition of the host cell Ca2+ ATPase (Ca2+ pump) and the ouabain-sensitive Na+/K+ ATPase (Na+/K+ pump). The Mg2+ ATPase which is not involved in cation transport was not affected. The presence of DIP in the inocula protected the cells from alteration of the transport-associated ATPases induced by standard viruses.
BAKERP. L. F.1972 Transport and metabolism of calcium ions in nerve. Progress in Biophysics and Molecular Biology24177–223 Edited by ButlerJ. A., NobleD. Oxford & New York: Pergamon Press;
DUTKOF. J., PFAUC. J.1978; Arenavirus defective interfering particles mask the cell-killing potential of standard virus. Journal of General Virology 38:195–208
FORMBYB., CAPITOK., EGEBERGJ., HEDESKOVC. J.1976; Ca2+-activated ATPase activity in subcellular fractions of mouse pancreatic islets. American Journal of Physiology 230:441–448
GIETZENK., ADAMCZYK-ENGELMANP., WÜTHRICHA., KONSTANTINOVAA., BAKERH.1983; Compound 48/80 is a selective and a powerful inhibitor of calmodulin-regulated functions. Biochimica et biophysica acta 736:109–118
GLYNNI. M.1985 The Na+, K+ transporting adenosine triphosphatase. The Enzymes of Biological Membranes, 2nd edn.35–144 Edited by MartonosiA. N. New York & London: Plenum Press;
GRONDAC, ROSSIJ. P. F. C, GAGLIARDINOJ. J.1987; Relationship between insulin secretion and rat islet plasma membrane Ca2+-ATPase during short-term fasting. Acta physiologica et pharmacologica latinoameri-cana 37:245–256
HAMLYNJ. M., SENIORA. E.1983; Evidence that Mg2+ or Ca2+ adenosine triphosphatase in rat pancreas is a plasma membrane ectoenzyme. Biochemical Journal 214:59–68
JORGENSENP. L., SKRIVERE., HEBERTH., MALINSBACHA. B.1982; Structure of the Na, K pump: crystallization of pure membrane-bound Na+, K+ ATPase and identification of functional domains of the α-subunit. Annals of the New York Academy of Sciences 402:207–225
LóPEZR., FRANZE-FERNÂNDEZM. T.1985; Effect of Tacaribe virus infection on host cell protein and nucleic acid synthesis. Journal of General Virology 66:1753–1761
LóPEZR., GRAUO., FRANZE-FERNÂNDEZM. T.1986; Effect of actinomycin D on arenavirus growth and estimation of the generation time for a virus particle. Virus Research 5:213–220
LOWRYO. H., ROSEBROUGHJ. H., FARRA. L., RANDALLR. J.1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
MORCOSM. C. 1982; Localization of (Ca2+ + Mg2+) ATPase Ca2+-pump and other ATPase activities in cardiac sarcolemma. Biochimica et biophysica acta 688:747–752
OLDSTONEM. B. A., HOLMSTOENI., WELSHR. E.1977; Alterations of acetylcholine enzymes in neuroblastoma cells persistently infected with lymphocytic choriomeningitis virus. Journal of Cellular Physiology 91:459–472
OLDSTONEM. B. A., SOUTHERNP., RODRIGUEZM., LAMPERTP. W.1984a; Virus persistence in the B cells of islet of Langerhans is associated with the chemical manifestation of diabetes. Science ITA,1440–1443
OLDSTONEM. B. A., RODRIGUEZM., DAUGHADAYW. H., LAMPERTP. W.1984b; Viral perturbations of endocrine functions: disordered cell functions leads to disturbed homeostasis and disease. Nature, London 307:278–281
PASTERNAKC A., MICKLEMK. J.1981; Virally induced alterations in cellular permeability: a basis of cellular and physiological damage?. Bioscience Reports 1:431–448
ROSSIJ. P. F. C, GARRAHANP. I., REGAA. F.1981; Vanadate inhibition of active Ca2+-transport across human red cell membranes. Biochimica et biophysica acta 648:145–150
ROSSIJ. P. F. C, REGAA. F., GARRAHANP. J.1985; Compound 48/80 and calmodulin modify the interaction of ATP with the (Ca2+ + Mg2+)-ATPase of red cell membranes. Biochimica et biophysica acta 816:379–386
ROUFOGALISB. D., AKYEMPONC. K., AL-JOBOREA., MINOCHERHOMJEEA. M.1982; Regulation of the Ca2+-pump of the erythrocyte membrane. Annals of the New York Academy of Sciences 402:349–366
SETHP., PASTANI., WILLINGHAMM. C. 1987; Adenovirus-dependent changes in cell membrane permeability: the role of Na+, K+ ATPase. Journal of Virology 61:883–888
VALSAMAKISA., RIVIEREY., OLDSTONEM. B. A.1987; Perturbation of differentiated functions in vivo during persistent viral infection. Virology 156:214–220