1887

Abstract

Summary

The complete nucleotide sequence of the RNA segment 10 of Australian BTV serotype 1 has been deduced from a combination of sequencing cDNA inserts cloned into pBR322 and synthetic deoxynucleotide priming on purified double-stranded RNA molecules. The gene segment was 822 nucleotides in length and capable of coding for two proteins on either 229 or 216 amino acids, having net charges of either +4.5 or +5.5 respectively at neutral pH. Comparison with the RNA segment 10 from BTV serotype 10 revealed a high degree of amino acid sequence conservation as well as regions of nucleic acid sequence conservation.

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/content/journal/jgv/10.1099/0022-1317-69-4-945
1988-04-01
2019-10-21
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-4-945
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