1887

Abstract

Summary

The complete nucleotide sequence of the RNA segment 10 of Australian BTV serotype 1 has been deduced from a combination of sequencing cDNA inserts cloned into pBR322 and synthetic deoxynucleotide priming on purified double-stranded RNA molecules. The gene segment was 822 nucleotides in length and capable of coding for two proteins on either 229 or 216 amino acids, having net charges of either +4.5 or +5.5 respectively at neutral pH. Comparison with the RNA segment 10 from BTV serotype 10 revealed a high degree of amino acid sequence conservation as well as regions of nucleic acid sequence conservation.

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/content/journal/jgv/10.1099/0022-1317-69-4-945
1988-04-01
2021-10-22
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References

  1. BASSEL-DUBY R., SPRIGGS D. R., TYLER K. L., FIELDS B. N. 1986; Identification of attenuating mutations on the reovirus type 3 S1 double-stranded RNA segment with a rapid sequencing technique. Journal of Virology 60:64–67
    [Google Scholar]
  2. CHEN E. Y., SEEBURG P. H. 1985; Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  3. EATON B. T., GOULD A. R. 1987; Isolation and characterization of Orbivirus genotypic variants. Virus Research 6:363–382
    [Google Scholar]
  4. GHIASI H., PURDY M. A., ROY P. 1985; The complete sequence of bluetongue virus serotype 10 segment 3 and its predicted VP3 polypeptide compared with those of BTV serotype 17. Virus Research 3:181–190
    [Google Scholar]
  5. GOULD A. R. 1987a; The complete nucleotide sequence of bluetongue virus serotype 1 RNA3 and a comparison with other geographic serotypes from Australia, South Africa and the United States of America, and with other Orbivirus isolates. Virus Research 7:169–183
    [Google Scholar]
  6. GOULD A. R. 1987b; Conserved and non-conserved regions of the outer coat protein, VP2, of the Australian bluetongue serotype 1 virus revealed by sequence comparison to the VP2 of North American BTV serotype 10. Virus Research in press
    [Google Scholar]
  7. GOULD A. R. 1988; The use of recombinant DNA probes to group and type orbiviruses. A comparison of Australian and South African isolates. Archives of Virology in press
    [Google Scholar]
  8. GOULD A. R., PRITCHARD L. I. 1987; The complete nucleotide sequence of the outer coat protein, VP5, of the Australian bluetongue serotype 1 reveals conserved and non-conserved sequences. Virus Research in press
    [Google Scholar]
  9. HUISMANS H., CLOETE M. 1987; A comparison of different cloned bluetongue virus genome segments as probes for the detection of virus-specified RNA. Virology 158:373–380
    [Google Scholar]
  10. HUISMANS H., ERASMUS B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
    [Google Scholar]
  11. INUMARU S., ROY P. 1987; Production and characterization of the neutralization antigen VP2 of bluetongue virus serotype 10 using a baculovirus expression vector. Virology 157:472–479
    [Google Scholar]
  12. KOWALIK T. F., LI J. K.-K. 1987; The genetic relatedness of United States prototype bluetongue virus by RNA/RNA hybridization. Virology 158:276–284
    [Google Scholar]
  13. KOZAK M. 1981; Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Research 9:5233–5252
    [Google Scholar]
  14. KOZAK M. 1984; Selection of initiation sites by eukaryotic ribosomes: effect of inserting AUG triplets upstream from the coding sequence of preproinsulin. Nucleic Acids Research 12:3873–3893
    [Google Scholar]
  15. LEE J. W., ROY P. 1986; Nucleotide sequence of a cDNA clone of RNA segment 10 of bluetongue virus (serotype 10). Journal of General Virology 67:2833–2837
    [Google Scholar]
  16. PURDY M. A., GHIASI H., RAO C. D., ROY P. 1985; Complete sequence of bluetongue virus L2 RNA that codes for the antigen recognized by neutralizing antibodies. Journal of Virology 55:826–830
    [Google Scholar]
  17. PURDY M. A., RITTER G. D., ROY P. 1986; Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10. Journal of General Virology 67:957–962
    [Google Scholar]
  18. SANGAR D. V., MERTENS P. P. C. 1983 Comparison of type 1 bluetongue virus protein synthesis in vivo and in vitro. Double-stranded RNA Viruses183–191 Edited by Compans R. W., Bishop D. H. L. New York: Elsevier;
    [Google Scholar]
  19. SANGER F., COULSON A. R. 1978; The use of thin acrylamide gels for DNA sequencing. FEBS Letters 87:107–110
    [Google Scholar]
  20. VERWOERD D. W., ELS H. I., DE VILLIERS E. M., HUISMANS H. 1972; Structure of the bluetongue virus capsid. Journal of Virology 10:783–794
    [Google Scholar]
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