1887

Abstract

Summary

Seven neutralizing monoclonal antibodies (MAbs) to the rotavirus simian agent 11 were produced. Although displaying variable degrees of haemagglutination-inhibiting activity, they were shown by radioimmunoprecipitation and Western blot analyses to react with the major outer capsid glycoprotein (VP7). In competition binding assays, MAbs defined two distinct VP7 epitopes, which appeared to be close to each other or partially overlapping. In addition, MAbs of the two epitope groups enhanced binding of a broadly reactive, non-neutralizing, MAb specific for rotavirus group antigen.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-4-937
1988-04-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/4/JV0690040937.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-4-937&mimeType=html&fmt=ahah

References

  1. ASTALDI O. C. B., JANSSEN M. C, LANSDORP P., WILLEMS C, ZEIJLEMAKER W. P., OOSTERHOF F. 1980; Human endothelial culture supernatant (HECS): a growth factor for hybridomas. Journal of Immunology 125:1411–1414
    [Google Scholar]
  2. BASTARDO J. W., MCKIMM-BRESCHKIN J. L., SONZA S., MERCER L. D., HOLMES I. H. 1981; Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides. Infection and Immunity 34:641–647
    [Google Scholar]
  3. CHAN W. K., PENARANDA M. E., CRAWFORD S. E., ESTES M. K. 1986; Two glycoproteins are produced from the rotavirus neutralization gene. Virology 151:243–252
    [Google Scholar]
  4. COULSON B. S., FOWLER K. J., BISHOP R. F., COTTON R. G. H. 1985; Neutralizing monoclonal antibodies to human rotavirus and indication of antigenic drift among strains from neonates. Journal of Virology 54:14–20
    [Google Scholar]
  5. COULSON B. S., TURSI J. M., MCADAM W. J., BISHOP R. F. 1986; Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. Virology 154:302–312
    [Google Scholar]
  6. DYALL-SMITH M. L., LAZDINS I., TREGEAR G. W., HOLMES I. H. 1986; Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proceedings of the National Academy of Sciences, U.S.A 83:3465–3468
    [Google Scholar]
  7. ESTES M. K., GRAHAM D. Y., MASON B. B. 1981; Proteolytic enhancement of rotavirus infectivity. molecular mechanisms. Journal of Virology 39:879–888
    [Google Scholar]
  8. FLORES J., MIDTHUN K., HOSHINO Y., GREEN K., GORZIGLIA M., KAPIKIAN A. Z., CHANOCK R. M. 1986; Conservation of the fourth gene among rotaviruses recovered from asymptomatic newborn infants and its possible role in attenuation. Journal of Virology 60:972–979
    [Google Scholar]
  9. GERNA G., BATTAGLIA M., MILANESI G., PASSARANI N., PERCIVALLE E., CATTANEO E. 1984; Serotyping of cell culture-adapted subgroup 2 human rotavirus strains by neutralization. Infection and Immunity 43:722–729
    [Google Scholar]
  10. GORZIGLIA M., HOSHINO Y., BUCKLER-WHITE A., BLUMENTALS I., GLASS R., FLORES J., KAPIKIAN A. Z., CHANOCK R. M. 1986; Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proceedings of the National Academy of Sciences, U.S.A 83:7039–7043
    [Google Scholar]
  11. GREENBERG H. B., FLORES I., KALICA A. R., WYATT R. G., JONES R. W. 1983a; Gene coding assignments for growth restriction, neutralization and subgroup specificities of W and DS-1 strains of human rotavirus. Journal of General Virology 64:313–320
    [Google Scholar]
  12. GREENBERG H. B., MCAULIFFE V., VALDESUSO J., WYATT R. G., FLORES J., KALICA A., HOSHINO Y., SINGH N. 1983b; Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infection and Immunity 39:91–99
    [Google Scholar]
  13. GREENBERG H. B., VALDESUSO J., VAN WYKE K., MIDTHUN K., WALSH M., MCAULIFFE V., WYATT R. G., KALICA A. R., FLORES J., HOSHINO Y. 1983c; Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. Journal of Virology 47:267–275
    [Google Scholar]
  14. HEATH R., BIRCH C., GUST I. 1986; Antigenic analysis of rotavirus isolates using monoclonal antibodies specific for human serotypes 1, 2, 3 and 4, and SA11. Journal of General Virology 67:2455–2466
    [Google Scholar]
  15. HOSHINO Y., SERENO M. M., MIDTHUN K., FLORES J., KAPIKIAN A. Z., CHANOCK R. M. 1985; Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proceedings of the National Academy of Sciences, U.S.A 82:8701–8704
    [Google Scholar]
  16. HSU S. M., RAINE L., FANGER H. 1981; The use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. Journal of Histochemistry and Cytochemistry 29:577–580
    [Google Scholar]
  17. KALICA A. R., JAMES H. D., KAPIKIAN A. Z. 1978; Hemagglutination by simian rotavirus. Journal of Clinical Microbiology 7:314–315
    [Google Scholar]
  18. KALICA A. R., GREENBERG H. B., WYATT R. G., FLORES J., SERENO M. M., KAPIKIAN A. Z., CHANOCK R. M. 1981; Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112:385–390
    [Google Scholar]
  19. KALICA A. R., FLORES J., GREENBERG H. B. 1983; Identification of a rotaviral gene that codes for hemagglutination and protease-enhançed plaque-formation. Virology 125:194–205
    [Google Scholar]
  20. LAZDINS I., SONZA S., DYALL-SMITH M. L., COULSON B. S., HOLMES I. H. 1985; Demonstration of an immunodominant neutralization site by analysis of antigenic variants of SA11 rotavirus. Journal of Virology 56:317–319
    [Google Scholar]
  21. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  22. NOVO E., ESPARZA J. 1981; Composition and topography of structural polypeptides of bovine rotavirus. Journal of General Virology 56:325–335
    [Google Scholar]
  23. OFFIT P. A., BLAVAT G. 1986; Identification of the two rotavirus genes determining neutralization specificities. Journal of Virology 57:376–378
    [Google Scholar]
  24. OFFIT P. A., BLAVAT G., GREENBERG H. B., CLARK H. F. 1986; Molecular basis of rotavirus virulence: role of gene segment 4. Journal of Virology 57:46–49
    [Google Scholar]
  25. SABARA M., GILCHRIST J. E., HUDSON G. R., BABIUK L. A. 1985; Preliminary characterization of an epitope involved in neutralization and cell attachment that is located on the major bovine rotavirus glycoprotein. Journal of Virology 53:58–66
    [Google Scholar]
  26. SHAW R. D., VO P. T., OFFIT P. A., COULSON B. S., GREENBERG H. B. 1986; Antigenic mapping of the surface proteins of rhesus rotavirus. Virology 155:434–451
    [Google Scholar]
  27. SONZA S., BRESCHKIN A. M., HOLMES I. H. 1983; Derivation of neutralizing monoclonal antibodies against rotavirus. Journal of Virology 45:1143–1146
    [Google Scholar]
  28. SONZA S., BRESCHKIN A. M., HOLMES I. H. 1984; The major surface glycoprotein of simian rotavirus (SA11) contains distinct epitopes. Virology 134:318–327
    [Google Scholar]
  29. TANIGUCHI K., URASAWA S., URASAWA T. 1985; Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. Journal of General Virology 66:1045–1053
    [Google Scholar]
  30. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  31. WILSON M. B., NAKANE P. K. 1978 Developments in the periodate method of conjugating horseradish peroxidase to antibodies. Immunofluorescence and Related Staining Techniques215–225 Edited by Knapp W. Amsterdam: Elsevier;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-4-937
Loading
/content/journal/jgv/10.1099/0022-1317-69-4-937
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error