1887

Abstract

Summary

Eight plant species that were believed to be resistant to mechanical inoculation with potato spindle tuber viroid (PSTV) were inoculated with virulent strains the recombinant Ti plasmids of which contained infectious dsDNA copies of PSTV. PSTV-related RNAs (including its circular and linear forms) were detected in galls and roots of the six species which developed crown galls; those which did not ( and ) did not contain detectable levels of PSTV-related RNA. None of the eight species contained detectable PSTV-related RNA in their foliage. Although PSTV appears to be stable and to be translocated in non-hosts, analysis of RNase-resistant dsRNA preparations from -inoculated sunflower suggests that PSTV does not undergo normal RNA-directed replication. -mediated inoculation of accession OCH11603 was followed by the appearance of PSTV in the foliage, suggesting that the previously reported resistance of this plant to PSTV infection is actually resistance to mechanical inoculation rather than immunity to infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-4-879
1988-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/4/JV0690040879.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-4-879&mimeType=html&fmt=ahah

References

  1. ANONYMOUS 1983 Annual Report-CIP 198355–63 Lima: International Potato Center;
    [Google Scholar]
  2. BARKER J. M., MCINNES J. L., MURPHY P. J., SYMONS R. H. 1985; Dot-blot procedure with [32P]DNA probes for the sensitive detection of avocado sunblotch and other viroids in plants. Journal of Virological Methods 10:87–98
    [Google Scholar]
  3. BEIER H., SILER D. J., RUSSELL M. L., BRUENING G. 1977; Survey of susceptibility to cowpea mosaic virus among protoplasts and intact plants from Vigna sinensis. Phytopathology 67:917–921
    [Google Scholar]
  4. BEIER H., BRUENING G., RUSSELL M. L., TUCKER C. L. 1979; Replication of cowpea mosaic virus in protoplasts isolated from immune lines of cowpea. Virology 95:165–175
    [Google Scholar]
  5. BRANCH A. D., ROBERTSON H. D. 1984; A replication cycle for viroids and other small infectious RNA’s. Science 223:450–455
    [Google Scholar]
  6. CRESS D. E., KIEFER M. C., OWENS R. A. 1983; Construction of infectious potato spindle tuber viroid cDNA clones. Nucleic Acids Research 11:6821–6835
    [Google Scholar]
  7. DHAESE P., DE GRèVE H., DECRAEMER H., SCHELL J., VAN MONTAGU M. 1979; Rapid mapping of transposon insertion and deletion mutations in the large Ti-plasmids of Agrobacterium tumefaciens. Nucleic Acids Research 7:1837–1849
    [Google Scholar]
  8. DIENER T. O. 1979 Viroids and Viroid Diseases New York: John Wiley & Sons;
    [Google Scholar]
  9. DIENER T. O., HADIDI A. 1977 Viroids. Comprehensive Virology 11285–337 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  10. FULTON R. W. 1964 Transmission of plant viruses by grafting, dodder, seed and mechanical inoculation. Plant Virology39–67 Edited by Corbett M. K., Sisler H. D. Gainesville: University of Florida Press;
    [Google Scholar]
  11. GARDNER R. C., KNAUF V. C. 1986; Transfer of Agrobacterium DNA to plants requires T-DNA border but not the virE locus. Science 231:725–727
    [Google Scholar]
  12. GARDNER R. C, CHONOLES K. R., OWENS R. A. 1986; Potato spindle tuber viroid infections mediated by the Ti plasmid of Agrobacterium tumefaciens. Plant Molecular Biology 6:221–228
    [Google Scholar]
  13. GRIMSLEY N., HOHN T., DAVIES J. W., HöHN B. 1987; Agrobacterium-mediated delivery of infectious maize streak virus into plants. Nature, London 325:177–179
    [Google Scholar]
  14. GROSS H. J., DOMDEY H., JANK P., LOSSOW C, RABA M., ALBERTY H., SANGER H. L. 1978; Nucleotide sequence and secondary structure of potato spindle tuber viroid. Nature, London 273:203–208
    [Google Scholar]
  15. HAMMOND R. W., OWENS R. A. 1987; Mutational analysis of potato spindle tuber viroid reveals complex relationships between structure and infectivity. Proceedings of the National Academy of Sciences, U.S.A 84:3967–3971
    [Google Scholar]
  16. HIEBERT E., BANCROFT J. B., BRACKER C. E. 1968; The assembly in vitro of some small spherical viruses, hybrid viruses and other nucleoproteins. Virology 34:492–508
    [Google Scholar]
  17. HUBER R., REZELMAN G., HIBI T., VAN KAMMEN A. 1977; Cowpea mosaic virus infection of protoplasts from Samsun tobacco leaves. Journal of General Virology 34:315–323
    [Google Scholar]
  18. HUTCHINS C. J., KEESE P., VISVADER J. E., RATHJEN P. D., MCINNES J. E., SYMONS R. H. 1985; Comparison of multimeric plus and minus forms of viroids and virusoids. Plant Molecular Biology 4:293–304
    [Google Scholar]
  19. KEESE P., SYMONS R. H. 1985; Domains in viroids: evidence of intermolecular RNA rearrangements and their contribution to viroid evolution. Proceedings of the National Academy of Sciences, U.S.A 82:4582–4586
    [Google Scholar]
  20. MCMASTER G. K., CARMICHAEL G. G. 1977; Analysis of single- and double-stranded nucleic acids on Polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A 74:4835–4838
    [Google Scholar]
  21. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. MATTHEWS R. E. F. 1981 Plant Virology, 2nd edn. New York & London: Academic Press;
    [Google Scholar]
  23. MELTON D. A., KRIEG P. A., REBAGLIATI M. R., MANIATIS T., ZINN K., GREEN M. R. 1984; Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Research 12:7035–7056
    [Google Scholar]
  24. MESSING J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:30–78
    [Google Scholar]
  25. MURRAY M., THOMPSON W. F. 1980; Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8:4321–4325
    [Google Scholar]
  26. ODELL J. T., NAGY F., CHU A N-H. 1985; Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature, London 313:810–812
    [Google Scholar]
  27. OWENS R. A., DIENER T. O. 1982; RNA intermediates in potato spindle tuber viroid replication. Proceedings of the National Academy of Sciences, U.S.A 79:113–117
    [Google Scholar]
  28. OWENS R. A., DIENER T. O. 1984; Spot hybridization for detection of viroids and viruses. Methods in Virology 7:173–187
    [Google Scholar]
  29. PALUKAITIS P. 1987; Potato spindle tuber viroid: investigation of the long distance intra-plant transport route. Virology 158:239–241
    [Google Scholar]
  30. RIESNER D. 1987 Physical-chemical properties: structure formation. The Viroids63–98 Edited by Diener T. O. New York & London: Plenum Press;
    [Google Scholar]
  31. RIESNER D., GROSS H. J. 1985; Viroids. Annual Review of Biochemistry 54:531–564
    [Google Scholar]
  32. SäNGER H. L., RAMM K. 1975 Radioactive labelling of viroids. Modification of the Information Content of Plant Cells229–252 Edited by Markham R., Davies D. R., Hopwood D. A., Horne R. W. Amsterdam: Elsevier;
    [Google Scholar]
  33. SHEWMAKER C. K., CATON J. R., HOUCK C. M., GARDNER R. C. 1985; Transcription of cauliflower mosaic virus integrated into plant genomes. Virology 140:281–288
    [Google Scholar]
  34. SINGH R. 1985; Clones of Solanum berthaultii resistant to potato spindle tuber viroid. Phytopathology 75:1432–1434
    [Google Scholar]
  35. TABLER M., SäNGER H. L. 1985; Infectivity studies on different potato spindle tuber viroid (PSTV) RNAs synthesized in vitro with the SP6 transcription system. EMBO Journal 4:2191–2199
    [Google Scholar]
  36. TURGEON R. 1982 Teratomas and secondary tumors. Molecular Biology of Plant Tumors391–414 Edited by Kahl G., Schell J. New York & London: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-4-879
Loading
/content/journal/jgv/10.1099/0022-1317-69-4-879
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error