1887

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Volume 69, Issue 4

Comparative Study on -linked Oligosaccharides of Glycoprotein D of Herpes Simplex Virus Types 1 and 2 Free

Abstract

Summary

Glycoproteins D1 (gD1) and D2 (gD2) of herpes simplex virus type 1 and type 2, respectively, were purified from infected HEp-2 cells labelled with [H]glucosamine for 14 h followed by a 3 h chase using HD1 monoclonal antibody linked to Sepharose. -linked oligosaccharides were found to be present in both glycoproteins. The identification of -acetyl [H]galactosaminitol as the major labelled component in the oligosaccharides generated by mild alkaline borohydride treatment demonstrated that these chains have -acetylgalactosamine at the reducing end. These oligosaccharides consist of mono- and disialylated species with a predominance of the latter species in gD1. Size analysis and radioactive amino sugar composition strongly suggest a structure in which the galactosyl--acetylgalactosamine core is substituted with one or two sialic acid residues. In terms of [H]glucosamine-derived radioactivity, -linked oligosaccharides are less represented than -linked oligosaccharides. The -linked oligosaccharide number determination showed that gD1 and gD2 carry two and three chains, respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): glycoprotein D , glycosylation , HSV and O-linked oligosaccharides
© Society for General Microbiology 1988
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1988-04-01
2024-04-20
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Comparative Study on O-linked Oligosaccharides of Glycoprotein D of Herpes Simplex Virus Types 1 and 2
J. Gen. Virol. 69, 869 (1988); https://doi.org/10.1099/0022-1317-69-4-869
/content/journal/jgv/10.1099/0022-1317-69-4-869
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