Glycoproteins D1 (gD1) and D2 (gD2) of herpes simplex virus type 1 and type 2, respectively, were purified from infected HEp-2 cells labelled with [H]glucosamine for 14 h followed by a 3 h chase using HD1 monoclonal antibody linked to Sepharose. -linked oligosaccharides were found to be present in both glycoproteins. The identification of -acetyl [H]galactosaminitol as the major labelled component in the oligosaccharides generated by mild alkaline borohydride treatment demonstrated that these chains have -acetylgalactosamine at the reducing end. These oligosaccharides consist of mono- and disialylated species with a predominance of the latter species in gD1. Size analysis and radioactive amino sugar composition strongly suggest a structure in which the galactosyl--acetylgalactosamine core is substituted with one or two sialic acid residues. In terms of [H]glucosamine-derived radioactivity, -linked oligosaccharides are less represented than -linked oligosaccharides. The -linked oligosaccharide number determination showed that gD1 and gD2 carry two and three chains, respectively.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error