1887

Abstract

Summary

The antibody response to herpes simplex virus (HSV) is complex and involves antibody to at least 33 virus-induced polyeptides. Serum IgG contains four isotypes in mice and it is known that the isotypes differ in their biological functions and that individual antigenic proteins may preferentially elicit restricted isotype responses. We therefore examined the anti-polypeptide isotypes induced in immune mouse serum. By ELISA, we found that the total serum virus-specific antibody activity was 51% IgG1, 39% IgG2a, 11% IgG2b and 1% IgG3 in immune ICR strain mice and 51%, 45%, 4% and 0.4% respectively in strain BALB/c mouse immune serum. These proportions are significantly different from those reported for other virus infections. Sepharose-Protein A affinity-purified isotypes were also studied and showed IgG1 > IgG2a ⩾ IgG2b ≫ IgG3 activity per µg of isotype, indicating that competition between isotypes present in high concentrations did not significantly alter the results. Immunoblotting studies of the purified isotypes showed that the major immunogenic HSV-1 proteins (VP155, gC, gB, pgB, gD and nucleocapsid proteins 42K and 44K) induced all isotypes. However, the isotype responses were not uniform among the glycoproteins and some other proteins. In addition neutralization assays of the purified isotypes indicated that IgG2a and IgG2b had significantly greater neutralizing capacity than IgG1, suggesting that less of the IgG1 was directed against neutralizing virion epitopes. These data are discussed with respect to the biological implications in host defence.

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1988-04-01
2024-11-07
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References

  1. ALMOND N. M., PARKHOUSE R. M. E. 1986; Immunoglobulin class specific responses to biochemically denned antigens of Trichinella spiralis. Parasite Immunology 8:391–406
    [Google Scholar]
  2. BARRETT D. J., AYOUB E. M. 1986; IgG2 subclass restriction of antibody to pneumococcal polysaccharides. Clinical and Experimental Immunology 63:127–134
    [Google Scholar]
  3. BECK O. E. 1981; Distribution of virus antibody activity among human IgG subclasses. Clinical and Experimental Immunology 43:626–632
    [Google Scholar]
  4. BUCKMASTER E. A., GOMPELS U., MINSON A. 1984; Characterization and physical mapping of an HSV-1 glycoprotein of approximately 115 × 103 molecular weight. Virology 139:408–413
    [Google Scholar]
  5. CHRISTIAANSEN J. E., SEARS D. W. 1984; Unusually efficient tumor cell lysis by human effectors of antibody-dependent cellular cytotoxicity mediated by monoclonal antibodies. Cancer Research 44:3712–3718
    [Google Scholar]
  6. COLEMAN R. M., NAHMIAS A. J., WILLIAMS S. C, PHILLIPS D. J., BLACK C M., REIMER C B. 1985; IgG Subclass antibodies to herpes simplex virus. Journal of Infectious Diseases 151:929–936
    [Google Scholar]
  7. COUTELIER J.-P., VAN SNICK J. 1985; Isotypically restricted activation of B lymphocytes by lactic dehydrogenase virus. European Journal of Immunology 15:250–255
    [Google Scholar]
  8. COUTELIER J.-P., VAN ROOST E., LAMBOTTE P., VAN SNICK J. 1986; The murine antibody response to lactate dehydrogenase-elevating virus. Journal of General Virology 67:1099–1108
    [Google Scholar]
  9. COUTELIER J.-P., VAN DER LOGT J. T. M., HEESSEN F. W. A., WARNIER G., VAN SNICK J. 1987; IgG2a restriction of murine antibodies elicited by viral infections. Journal of Experimental Medicine 165:64–69
    [Google Scholar]
  10. DER BALIAN G. P., SLACK J., CLEVINGER B. L., BAZIN H., DAVIE J. M. 1980; Subclass restriction of murine antibodies. III. Antigens that stimulate IgG2 in mice stimulate IgG2c in rats. Journal of Experimental Medicine 152:209–218
    [Google Scholar]
  11. DJURUP R., WEEKE B. 1986 Methods of detecting IgG subclass proteins and antibodies. Basic and Clinical Aspects of IgG Subclasses91 Edited by Shakib F. Basel: S. Karger;
    [Google Scholar]
  12. EBERLE R., MOU S-W., ZAIA J. A. 1985; The immune response to herpes simplex virus: comparison of the specificity and relative titers of serum antibodies directed against viral polypeptides following primary herpes simplex virus type 1 infections. Journal of Medical Virology 16:147–162
    [Google Scholar]
  13. EY P. L., PROWSE S. J., JENKINS C. R. 1978; Isolation of pure IgG I, IgG2a and IgG2b immunoglobulins from mouse serum using ProteinA-Sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  14. GILLJAM G., SUNDQVIST V.-A., LINDE A., PIHLSTEDT P., EKLUND A. E., WAHREN B. 1985; Sensitive analytic ELISAs for subclass herpes virus IgG. Journal of Virological Methods 10:203–214
    [Google Scholar]
  15. HAMMARSTROM L., SMITH C. I. E. 1986 IgG subclasses in bacterial infections. Basic and Clinical Aspects of IgG Subclasses122–133 Edited by Shakib F. Basel: S. Karger;
    [Google Scholar]
  16. HEILMAN C. J., ZWEIG M., HAMPAR B. 1981; Herpes simplex virus types 1 and 2 intracellular p40: type-specific and cross-reactive antigenic determinants on peptides generated by partial proteolysis. Journal of Virology 40:508–515
    [Google Scholar]
  17. HERLYN D., POWE J., ROSS A. H., HERLYN M., KOPROWSKI H. 1985; Inhibition of human tumor growth by IgG2a monoclonal antibodies correlates with antibody density on tumor cells. Journal of Immunology 134:1300–1304
    [Google Scholar]
  18. ISAKSON P. C. 1986; Antiimmunoglobulin-treated B cells respond to a B cell differentiation factor of IgG1. Journal of Experimental Medicine 164:303–308
    [Google Scholar]
  19. ISAKSON P. C, PURE E., VITETTA E., KRAMMER P. 1982; T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells. Journal of Experimental Medicine 155:734–748
    [Google Scholar]
  20. JOHNSON W. J., STEPLEWSKI Z., MATTHEWS T. J., HAMILTON T. A., KOPROWSKI H., ADAMS D. O. 1986; Cytolytic interactions between murine macrophages, tumor cells, and monoclonal antibodies: characterization of lytic conditions and requirements for effector activation. Journal of Immunology 136:4704–4713
    [Google Scholar]
  21. KIPPS T. J., PARHAM P., PUNT J., HERZENBERG L. A. 1985; Importance of immunoglobulin isotype in human antibody-dependent, cell-mediated cytotoxicity directed by murine monoclonal antibodies. Journal of Experimental Medicine 161:1–17
    [Google Scholar]
  22. KLAUS G. G. B., PEPYS M. B., KITAJIMA K., ASKONAS B. A. 1979; Activation of mouse complement by different classes of mouse antibody. Journal of Immunology 38:687–695
    [Google Scholar]
  23. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  24. LANGLOIS A. J., MATTHEWS T. J., WEINHOLD K. J., BOLOGNESI D. P. 1985; Immunologic control of a retrovirus-associated murine adenocarcinoma. VII. Tumor cell destruction by macrophages and IgG2a. Journal of the National Cancer Institute 75:709–715
    [Google Scholar]
  25. LEE S. I., HEINER D. C., WARA D. 1986 Development of serum IgG subclass levels in children. Basis and Clinical Aspects of IgG Subclasses108–121 Edited by Shakib F. Basel: S. Karger;
    [Google Scholar]
  26. LINDE G. A., HAMMARSTROM L., PERSSON M. A. A., SMITH C. I. E., SUNDQVIST V.-A., WAHREN B. 1983; Virus-specific antibody activity of different subclasses of immunoglobulins G and A in cytomegalovirus infections. Infection and Immunity 42:237–244
    [Google Scholar]
  27. MCDONALD T. L., DONNELLY T., WEBER A., QUENETTE L. 1983; Antibody classes and subclasses in circulating immune complexes isolated from mice infected with lactic dehydrogenase virus. Journal of Immunology 48:511–517
    [Google Scholar]
  28. MCKENDALL R. R. 1983; Delayed IgG-mediated clearance of herpes simplex virus type 1 from the CNS but not footpad during the early stages of infection: possible result of relative integrity of the blood-brain barrier. Journal of General Virology 64:1965–1972
    [Google Scholar]
  29. MCKENDALL R. R., PETIT M., WOO W. 1988a; The immunoglobulin response to individual HSV-1 viral proteins: determination of immunogenic proteins and relative antibody titer to individual polypeptides by immunoblotting. Journal of Medical Microbiology 25:49–57
    [Google Scholar]
  30. MCKENDALL R. R., PETIT M., WOO W. 1988b; The immunological response to individual HSV-1 viral polypeptides: kinetics of the response during primary and secondary experimental infection with herpes simplex virus. Journal of Medical Microbiology 25:59–66
    [Google Scholar]
  31. MATSUI H., MOROYAMA T., MENDELSOHN J. 1986; Mechanism of antitumor activity in mice for anti-epidermal growth factor receptor monoclonal antibodies with different isotypes. Cancer Research 46:5592–5598
    [Google Scholar]
  32. MICHAELIDES M. C., SIMMS E. S. 1977; Immune responses in mice infected with lactic dehydrogenase virus. I. Antibody response to DNP-BGG and hyperglobulinaemia in BALB/c mice. Journal of Immunology 32:981–988
    [Google Scholar]
  33. MONGINI P. K. A., PALEL W. E., METCALF E. S. 1982; T cell regulation of immunoglobulin class expression in the antibody response to trinitrophenyl-ficoll: evidence for T cell enhancement of the immunoglobulin class switch. Journal of Experimental Medicine 155:884–902
    [Google Scholar]
  34. MORTIMER G. E., WIDDOWSON J. P. 1979; Predominance of immunoglobulin G subclass 3 among the complement-fixing antibodies to streptococcal m-associated protein. Clinical and Experimental Immunology 37:247–258
    [Google Scholar]
  35. NATSUUME-SAKAI S., MOTONISHI K., MIGITA S. 1977; Quantitative estimations of five classes of immunoglobulin in inbred mouse strains. Immunology 32:861–866
    [Google Scholar]
  36. PERLMUTTER R. M., HANSBURG D., BRILES D. E., NICOLOTTI R. A., DAVIE J. M. 1978; Subclass restriction of murine anti-carbohydrate antibodies. Journal of Immunology 121:566–572
    [Google Scholar]
  37. RADBRUCH A., BURGER C, KLEIN S., MULLER W. 1986; Control of immunoglobulin class switch recombination. Immunological Reviews 89:69–83
    [Google Scholar]
  38. RAUTONEN N., PELKONEN J., SIPINEN S., KAYHTY H., MAKELA O. 1986; Isotype concentrations of human antibodies to group A meningococcal polysaccharide. Journal of Immunology 137:2670–2675
    [Google Scholar]
  39. REALE M. A., BONA C. A., SCHULMAN J. L. 1985; Isotype profiles of anti-influenza antibodies in mice bearing the xid defect. Journal of Virology 53:425–429
    [Google Scholar]
  40. SIDERAS P., BERGSTEDT-LINDQVIST S., SEVERINSON E. 1985; Partial biochemical characterization of IgG1-inducing factor. European Journal of Immunology 15:593–598
    [Google Scholar]
  41. SKVARIL F. 1986 IgG subclasses in viral infections. Basic and Clinical Aspects of IgG Subclasses134–143 Edited by Shakib F. Basel: S. Karger;
    [Google Scholar]
  42. STEPLEWSKI Z., SPIRA G., BLASZCZYK M., LÜBECK M. D., RADBRUCH A., ILLGES H., HERLYN D., RAJEWSKY K., SCHARFF M. 1985; Isolation and characterization of anti-monosialoganglioside monoclonal antibody 19-9 class-switch variants. Proceedings of the National Academy of Sciences, U.S.A 82:8653–8657
    [Google Scholar]
  43. STEVENS R., DICHEK D., KELD B., HEINER D. 1983; IgG1 is the predominant subclass of in vivo- and in vitro-produced anti-tetanus toxoid antibodies and also serves as the membrane IgG molecule for delivering inhibitory signals to anti-tetanus toxoid antibody-producing B cells. Journal of Clinical Immunology 3:65–69
    [Google Scholar]
  44. TARTAKOVSKY B., DE BAETSELIER P., ISAKOV N., KATSAV S., FELDMAN M., SEGAL S. 1983; ClaSS I MHC antigens of different subregions induce different IgG subclasses. Journal of Immunology 130:1017–1018
    [Google Scholar]
  45. TEALE J. M., ABRAHAM K. M. 1987; The regulation of antibody class expression. Immunology Today 8:122–127
    [Google Scholar]
  46. TESCH H., MULLER W., RAJEWSKY K. 1986; Lymphokines regulate immunoglobulin isotype expression in an antigen-specific immune response. Journal of Immunology 136:2892–2895
    [Google Scholar]
  47. THOMSEN A. R., VOLKERT M., MARKER O. 1985; Different isotype profiles of virus-specific antibodies in acute and persistent lymphocytic choriomeningitis virus infection in mice. Journal of Immunology 55:213–223
    [Google Scholar]
  48. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  49. YOUNT W. J., DORNER M. M., KUNKEL H. G., KABAT E. A. 1968; Studies on human antibodies. VI. Selective variations in subgroup composition and genetic markers. Journal of Experimental Medicine 127:633–646
    [Google Scholar]
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