Characterization of Attenuated Strains of Rift Valley Fever Virus Free

Abstract

Summary

The wild-type ZH501 strain of Rift Valley fever (RVF) virus and two small-plaque strains (T1 and T46) derived from it were characterized by plaque size, pathogenicity for hamsters and ability to replicate in Vero cells. Additionally, a mutagenized, attenuated, large-plaque, vaccine-candidate strain of RVF virus (ZH548-M12) was also studied. Infections with either the ZH501 or T46 strain were uniformly fatal to hamsters. In contrast, nearly all hamsters infected with either the T1 or ZH548-M12 strains survived and were immune to challenge with 10 LD of the ZH501 strain. Both of these attenuated strains failed to replicate in Vero cells maintained at 41 °C, whereas the more virulent strains (ZH501 and T46) replicated at this temperature. The low virulence and ability to induce protection against lethal RVF virus challenge that is associated with the T1 and ZH548-M12 strains make them potential vaccine candidates.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-4-817
1988-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/4/JV0690040817.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-4-817&mimeType=html&fmt=ahah

References

  1. BOYLE J. J. 1967; Biological characteristics of plaque variants of Rift Valley fever virus. American Journal of Veterinary Research 28:1027–1031
    [Google Scholar]
  2. CAPLEN H., PETERS C. J., BISHOP D. H. L. 1985; Mutagen-directed attenuation of Rift Valley fever virus as a method for vaccine development. Journal of General Virology 66:2271–2277
    [Google Scholar]
  3. COETZER J. A. W., BARNARD B. J. H. 1977; Hydrops amnii in sheep associated with hydranencephaly and arthrogryposis with Wesselbron disease and Rift Valley fever viruses as aetiological agents. Onderstepoort Journal of Veterinary Research 44:119–126
    [Google Scholar]
  4. EARLEY E., PERALTA P. H., JOHNSON K. M. 1967; A plaque neutralization method for arboviruses. Proceedings of the Society for Experimental Biology and Medicine 125:741–747
    [Google Scholar]
  5. GARGAN T. P. II, BAILEY C. L., HIGBEE G. A., GAD A., EL SAID S. 1983; The effect of laboratory colonization on the vector-pathogen interactions of Egyptian Culex pipiens and Rift Valley fever virus. American Journal of Tropical Medicine and Hygiene 32:1154–1163
    [Google Scholar]
  6. MEEGAN J. M. 1979; The Rift Valley fever epizootic in Egypt 1977-1978. 1. Description of the epizootic and virological studies. Transactions of the Royal Society of Tropical Medicine and Hygiene 73:618–623
    [Google Scholar]
  7. MORRILL J. C, JENNINGS G. B., CAPLEN H., TURELL M. J., JOHNSON A. J., PETERS C. J. 1987; Pathogenicity and immunogenicity of a mutagen-attenuated Rift Valley fever virus immunogen in pregnant ewes. American Journal of Veterinary Research 48:1042–1047
    [Google Scholar]
  8. MOUSSA M. I., WOOD O. L., ABDEL WAHAB K. S. E. 1982; Reduced pathogenicity associated with a small plaque variant of the Egyptian strain of Rift Valley fever virus (ZH501). Transactions of the Royal Society of Tropical Medicine and Hygiene 76:482–486
    [Google Scholar]
  9. PETERS C. J., ANDERSON G. W. JR 1981 Pathogenesis of Rift Valley fever. Contributions to Epidemiology and Biostatistics 321–41 Edited by Swartz T. A., Klingberg M. A., Goldblum N., Papier C. M. Basel: S. Karger;
    [Google Scholar]
  10. REED L. J., MUENCH H. 1938; A simple method for estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  11. SMITHBURN K. C. 1949; Rift Valley fever: the neurotropic adaption of virus and experimental use of this modified virus as a vaccine. British Journal of Experimentai Pathology 30:1–16
    [Google Scholar]
  12. VAN HOOSIER G. L. JR, LADIGES W. C. 1984 Biology and diseases of hamsters. Laboratory Animal Medicine123–147 Edited by Fox J., Cohen B., Loew F. New York & London: Academic Press;
    [Google Scholar]
  13. WEISS K. E. 1962; Studies on Rift Valley fever. Passive and active immunity in lambs. Onderstepoort Journal of Veterinary Research 29:3–9
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-4-817
Loading
/content/journal/jgv/10.1099/0022-1317-69-4-817
Loading

Data & Media loading...

Most cited Most Cited RSS feed