1887

Abstract

Summary

Bovine leukaemia virus (BLV) induces syncytia in productively infected ovine and bovine monolayer cells. Expression of the gene directly determines the syncytium-forming activity, since the expression of a cloned gene directed by the simian virus 40 (SV40) early promoter efficiently induced syncytia in transfected ovine embryonic (OE) cells. However a BLV long terminal repeat (LTR)-directed expression plasmid (pLTRenv) failed to induce syncytia in transfected OE cells, suggesting insufficient promoter activity of the LTR sequences. To assess the role of the XBL genes, which are located in the 3′ distal region of the genome, in viral gene expression we constructed SV40 early promoter-directed expression plasmids. These contained open reading frames (ORFs) in the XBL region, and were examined for syncytium-inducing activity by cotransfection with pLTRenv. The results suggest that both XBL-I (the longest ORF: ) and XBL-II (a shorter overlapping ORF: ) are -acting genes which cooperatively activate LTR-directed viral gene expression.

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/content/journal/jgv/10.1099/0022-1317-69-4-797
1988-04-01
2022-08-10
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References

  1. BURNY A., BRUCK C, CHANTRENNE H., CLEUTER Y., DEKEGEL D., GHYSDAEL J., KETTMANN R., LECLERCQ M., MAMMERICKX M., PORTETELLE D. 1980 Bovine leukemia virus: molecular biology and epidemiology. Viral Oncology231–289 Edited by Klein G. New York: Raven Press;
    [Google Scholar]
  2. CANN A. J., ROSENBLATT J. D., WACHSMAN W., SHAH N. P., CHEN I. S. Y. 1985; Identification of the gene responsible for human T-cell leukaemia virus transcriptional regulation. Nature, London 318:571–574
    [Google Scholar]
  3. FELBER B. K., PASKALIS H., KLEINMAN-EWING C, WONG-STAAL F., PAVLAKIS G. N. 1985; The pX protein of HTLV-1 is a transcriptional activator of its long terminal repeats. Nature, London 229:675–679
    [Google Scholar]
  4. GRAHAM F. L., VAN DER EB A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  5. HASELTINE W. A., SODROSKI J., PATARCA R., BRIGGS D., PERKINS D., WONG-STAAL F. 1984; Structure of 3′-terminal region of type II human T lymphotropic virus: evidence for new coding region. Science 225:419–421
    [Google Scholar]
  6. INOUE J., YOSHIDA M., SEIKI M. 1987; Transcriptional (p40x) and post-transcriptional (p27xln) regulators are required for the expression and replication of human T-cell leukemia virus type I genes. Proceedings of the National Academy of Sciences, U.S.A 84:3653–3657
    [Google Scholar]
  7. ITOHARA S., SEKIKAWA K. 1987; Molecular cloning of infectious proviral genomes of bovine leukemia virus. Virology 159:158–160
    [Google Scholar]
  8. KATOH I., YOSHINAKA Y., SAGATA N., IKAWA Y. 1987; The bovine leukemia virus X region encodes a trans-activator of its long terminal repeat. Japanese Journal of Cancer Research (Gann 78:93–98
    [Google Scholar]
  9. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. NAGASHIMA K., YOSHIDA M., SEIKI M. 1987; A single species of pX mRNA of human T-cell leukemia virus type I encodes trans-activator p40x and two other phosphoproteins. Journal of Virology 60:394–399
    [Google Scholar]
  11. RICE N. R., SIMEK S. L., DUBOIS G. C, SHOWALTER S. D., GILDEN R. V., STEPHENS R. M. 1987; Expression of the bovine leukemia virus X region in virus-infected cells. Journal of Virology 61:1577–1585
    [Google Scholar]
  12. ROSEN C. A., SODROSKI J. G., WILLEMS L., KETTMANN R., CAMPBELL K., ZAYA R., BURNY A., HASELTINE W. A. 1986; The 3′ region of bovine leukemia virus genome encodes a trans-activator protein. EMBO Journal 5:2585–2589
    [Google Scholar]
  13. SAGATA N., TSUZUKU-KAWAMURA J., NAGAYOSHI-AIDA M., SHIMIZU F., IMAGAWA K-I., IKAWA Y. 1985a; Identification and some biochemical properties of the major XBL gene product of bovine leukemia virus. Proceedings of the National Academy of Sciences. U.S.A 82:7879–7883
    [Google Scholar]
  14. SAGATA N., YASUNAGA Y., IKAWA Y. 1985b; Two distinct polypeptides may be translated from a single spliced mRNA of the X genes of human T-cell leukemia and bovine leukemia viruses. FEBS Letters 192:37–42
    [Google Scholar]
  15. SAGATA N., YASUNAGA Y., TSUZUKU-KAWAMURA J., OHISHI K., OGAWA Y., IKAWA Y. 1985c; Complete nucleotide sequence of the genome of bovine leukemia virus: its evolutionary relationship to other retroviruses. Proceedings of the National Academy of Sciences, U.S.A 82:677–681
    [Google Scholar]
  16. SEIKI M., HATTORI S., HIRAYAMA Y., YOSHIDA M. 1983; Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proceedings of the National Academy of Sciences, U.S.A 80:3618–3622
    [Google Scholar]
  17. SEIKI M., HIKIKOSHI A., TANIGUCHI T., YOSHIDA M. 1985; Expression of the pX gene of HTLV-1: general splicing mechanism in the HTLV family. Science 228:1532–1534
    [Google Scholar]
  18. SEIKI M., INOUE J., TAKEDA T., YOSHIDA M. 1986; Direct evidence that p40x of human T-cell leukemia virus type I is a rrans-acting transcriptional activator. EMBO Journal 5:561–565
    [Google Scholar]
  19. SHIMOTOHNO K., WACHSMAN W., TAKAHASHI Y., GOLDE D. W., MIWA M., SUGIMURA T., CHEN I. S. Y. 1984; Nucleotide sequence of the 3′ region of an infectious human T-cell leukemia virus type II genome. Proceedings of the National Academy of Sciences, U.S.A 81:6657–6661
    [Google Scholar]
  20. SODROSKI J., ROSEN C, GOH W. C., HASELTINE W. A. 1985; A transcriptional activator protein encoded by the x-lor region of the human T-cell leukemia virus. Science 228:1430–1434
    [Google Scholar]
  21. SOUTHERN P. J., BERG P. 1982; Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. Journal of Molecular and Applied Genetics 1:327–341
    [Google Scholar]
  22. STOW N. D., WILKIE N. M. 1976; An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA. Journal of General Virology 33:447–458
    [Google Scholar]
  23. WACHSMAN W., GOLDE D. W., TEMPLE P. A., ORR E. C, CLARK S. C., CHEN I. S. Y. 1985; HTLV X-gene product: requirement for the env methionine initiation codon. Science 228:1534–1537
    [Google Scholar]
  24. WONG-STAAL F., GALLO R. C. 1985; Human T-lymphotropic retroviruses. Nature, London 317:395–403
    [Google Scholar]
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