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Infection of cowpea (Vigna unguiculata) cells or protoplasts with cowpea mosaic virus is accompanied by the appearance of characteristic cytopathic structures. A major constituent of these cytopathic structures, represented in thin sections by electrondense material, was shown to contain viral non-structural proteins by its reaction with antisera to non-structural proteins and their detection with colloidal gold-labelled Protein A. With this same technique virus particles were found to accumulate mainly in the cytoplasm.
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