1887

Abstract

Summary

Infection of cowpea () cells or protoplasts with cowpea mosaic virus is accompanied by the appearance of characteristic cytopathic structures. A major constituent of these cytopathic structures, represented in thin sections by electrondense material, was shown to contain viral non-structural proteins by its reaction with antisera to non-structural proteins and their detection with colloidal gold-labelled Protein A. With this same technique virus particles were found to accumulate mainly in the cytoplasm.

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/content/journal/jgv/10.1099/0022-1317-69-3-751
1988-03-01
2023-01-27
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References

  1. ASSINK A. M., SWAANS H., VAN KAMMEN A. 1973; The localisation of virus-specific double-stranded RNA of cowpea mosaic virus in subcellular fractions of infected Vigna leaves. Virology 53:384–391
    [Google Scholar]
  2. DE ZOETEN G. A., ASSINK A. M., VAN KAMMEN A. 1974; Association of cowpea mosaic virus-induced double-stranded RNA with a cytopathological structure in infected cells. Virology 59:341–355
    [Google Scholar]
  3. DORSSERS L., VAN DER KROL S., VAN DER MEER J., VAN KAMMEN A., ZABEL P. 1984; Purification of cowpea mosaic virus RNA replication complex: identification of a virus-encoded 110,000 dalton polypeptide responsible for chain elongation. Proceedings of the National Academy of Sciences, U.S.A 81:1951–1955
    [Google Scholar]
  4. GOLDBACH R., VAN KAMMEN A. 1985 Structure, replication and expression of the bipartite genome of cowpea mosaic virus. Molecular Plant Virology 283–120 Edited by Davies J. W. Boca Raton: CRC Press;
    [Google Scholar]
  5. JAEGLE M., WELLINK J., GOLDBACH R. 1987; The genome-linked protein of cowpea mosaic virus is bound to the 5′ terminus of virus RNA by a phosphodiester linkage to serine. Journal of General Virology 68:627–632
    [Google Scholar]
  6. MAULE A. J., BOULTON M. I., EDMUNDS C., WOOD K. R. 1980; Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. Journal of General Virology 47:199–203
    [Google Scholar]
  7. REZELMAN G., GOLDBACH R., VAN KAMMEN A. 1980; Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts. Journal of Virology 36:366–373
    [Google Scholar]
  8. REZELMAN G., FRANSSEN H. J., GOLDBACH R. W., IE T. S., VAN KAMMEN A. 1982; Limits to the independence of bottom component RNA of cowpea mosaic virus. Journal of General Virology 60:335–342
    [Google Scholar]
  9. TOMENIUS K., CLAPHAM D., OXELFELT P. 1983; Localization by immunogold cytochemistry of viral antigen in sections of plant cells infected with red clover mottle virus. Journal of General Virology 64:2669–2678
    [Google Scholar]
  10. VAN KAMMEN A., EGGEN H. I. L. 1986; The replication of cowpea mosaic virus. BioEssays 5:261–266
    [Google Scholar]
  11. VAN LENT J. W. M., VERDUIN B. J. M. 1986 Detection of viral protein and particles in thin sections of infected plant tissue using immunogold labelling. Developments in Applied Biology I. Developments and Applications in Virus Testing193–211 Edited by Jones R. A. C., Torrance L. Wellesbourne: Association of Applied Biologists;
    [Google Scholar]
  12. WELLINK J., JAEGLE M., GOLDBACH R. 1987; Detection of a novel protein encoded by the B RNA of cowpea mosaic virus, using antibodies raised against a synthetic peptide. Journal of Virology 61:236–238
    [Google Scholar]
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