1887

Abstract

Summary

Double-stranded cDNA copies of RNA-1 and RNA-2 of tomato ringspot virus were cloned into pUC9. Comparison of restriction enzyme maps of eight clones indicated that 1.9 kb at the 3′ termini of RNA-1 and RNA-2 were similar. Five of these clones were used in Northern hybridization analyses and found to contain sequences either unique to RNA-1 or RNA-2, or common to RNA-1 and RNA-2. Southern blot analyses using clones derived from RNA-1 and RNA-2 confirmed that there is a 1.9 kb nucleotide sequence homology at the 3′ termini. A sequence homology of this magnitude has not been reported previously for other plant viruses with multipartite ssRNA genomes.

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/content/journal/jgv/10.1099/0022-1317-69-3-745
1988-03-01
2019-12-05
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-3-745
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