1887

Abstract

Summary

The synthesis of caprine arthritis-encephalitis virus structural proteins was analysed in infected cells labelled with [S]methionine and [H]glucosamine and by translation of virion RNA . Viral polypeptides were isolated from infected cell lysates or from translation products by immunoprecipitation with specific antisera and resolved by SDS-PAGE. Results indicated that the gene-encoded p28, p19 and p16 virion core proteins were formed by cleavage processing of a 55K precursor with several intermediate polypeptides. The gp135 virion surface glycoprotein, encoded by the gene, was formed by post-translational modification of a glycosylated precursor of 150K apparent . This precursor was formed by glycosylation of a 90K primary gene product.

Keyword(s): CAEV , lentivirus and structural proteins
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/content/journal/jgv/10.1099/0022-1317-69-3-675
1988-03-01
2021-10-22
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References

  1. ADAMS D. S., GOGOLEWSKI R. P., BARBET A. F., CHEEVERS W. P. 1985; Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines. Journal of General Virology 66:139–143
    [Google Scholar]
  2. CHEEVERS W. P., ROBERSON S. M., KLEVJER-ANDERSON P., CRAWFORD T. B. 1981; Characterization of caprine arthritis-encephalitis virus: a retrovirus of goats. Archives of Virology 67:111–117
    [Google Scholar]
  3. CRAWFORD T. B., ADAMS D. S., CHEEVERS W. P., CORK L. C. 1980; Chronic arthritis in goats caused by a retrovirus. Science 207:997–999
    [Google Scholar]
  4. DIGGELMAN H. 1979; Biosynthesis of an unglycosylated envelope glycoprotein of Rous sarcoma virus in the presence of tunicamycin. Journal of Virology 30:799–804
    [Google Scholar]
  5. DRESLER S., RUTA M., MURRAY M. J., KABAT D. 1979; Glycoprotein encoded by the Friend spleen focus-forming virus. Journal of Virology 30:564–575
    [Google Scholar]
  6. GOGOLEWSKI R. P., ADAMS D. S., McGULRE T. C, BANKS K. L., CHEEVERS W. P. 1985; Antigenic cross-reactivity between caprine arthritis-encephalitis, visna and progressive pneumonia viruses involves all virion-associated proteins and glycoproteins. Journal of General Virology 66:1233–1240
    [Google Scholar]
  7. JACKS T., VARMUS H. E. 1985; Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting. Science 230:1237–1242
    [Google Scholar]
  8. JACOBSON M. F., BALTIMORE D. 1968; Polypeptide cleavages in the formation of poliovirus proteins. Proceedings of the National Academy of Sciences, U.S.A 61:77–84
    [Google Scholar]
  9. JOHNSON G. C, BARBET A. F., KLEVJER-ANDERSON P., McGUIRE T. C. 1983; Preferential immune response to virion surface glycoproteins by caprine arthritis-encephalitis virus-infected goats. infection and Immunity 41:657–665
    [Google Scholar]
  10. KLEVJER-ANDERSON P., CHEEVERS W. P. 1981; Characterization of the infection of caprine synovial membrane cells by the retrovirus caprine arthritis-encephalitis virus. Virology 110:113–119
    [Google Scholar]
  11. KLEVJER-ANDERSON P., McGUIRE T. C. 1982; Neutralizing antibody response of rabbits and goats to caprine arthritis-encephalitis virus. Infection and Immunity 38:455–461
    [Google Scholar]
  12. PYPER J. M., CLéMENTS J. E., GONDA M. A., NARAYAN O. 1986; Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses. Journal of Virology 58:665–670
    [Google Scholar]
  13. ROBERSON S. M., CHEEVERS W. P. 1982; Purification of retrovirus genomic RNA suitable for chemical radioiodination. Journal of Virological Methods 4:315–321
    [Google Scholar]
  14. SCHOCHETMAN G., LONG C. W., OROSZLAN S., ARTHUR L., FINE D. L. 1978; Isolation of separate precursor polypeptides to mouse mammary tumor virus glycoproteins and nonglycoproteins. Virology 85:168–174
    [Google Scholar]
  15. SHAPIRO S. Z., STRAND M., AUGUST J. R. 1976; High molecular weight precursor polypeptides to structural proteins of Rauscher murine leukemia virus. Journal of Molecular Biology 107:459–477
    [Google Scholar]
  16. SONIGO P., ALIZON M., STASKUS K., KLATZMANN D., COLE S., DANOS O., RETZEL E., TIOLLAIS P., HAASE A., WAIN-HOBSON S. 1985; Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus. Cell 42:369–382
    [Google Scholar]
  17. VIGNE R., FLLIPPI P., QUERAT G., SAUZE N., VITU C, RUSSO P., DELORI P. 1982; Precursor polypeptides to structural proteins of visna virus. Journal of Virology 42:1046–1056
    [Google Scholar]
  18. WEISS S. R., HACKETT P. B., OPPERMANN H., ULLRICH A., LEVINTOW L., BISHOP J. M. 1978; Cell-free translation of avian sarcoma virus RNA: suppression of the gag termination codon does not augment synthesis of the joint gag/pol product. Cell 15:607–614
    [Google Scholar]
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