1887

Abstract

Summary

Equine infectious anaemia virus is related by genome sequence homology to human immunodeficiency virus, caprine arthritis-encephalitis virus and visna virus. Failure of the host to mount a strong neutralizing response detectable or to eliminate persistent infection characterizes lentivirus infections in the natural host. In this study the specificities and neutralizing activity of antibodies induced during experimental infection with equine infectious anaemia virus were investigated using antiviral ELISA, radioimmunoprecipitation and neutralization assays. ELISA antibody titres of 10 to 10 were demonstrated in samples collected 30 and 60 days after infection. Immunoprecipitation titrations demonstrated that antibody titres to the glycoproteins gp90 and gp45 were 10 to 100 times higher than titres to the internal structural protein, p24. Low levels of neutralizing antibody appeared at 23 to 46 days post-infection. The presence of low levels of neutralizing activity in the presence of high levels of anti-glycoprotein activity suggests that the major immunogenic sites on the viral surface are not sensitive to neutralization.

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1988-03-01
2022-08-15
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References

  1. ADAMS D. S., GORHAM J. R. 1986; The gpl35 of caprine arthritis-encephalitis virus affords greater sensitivity than the p28 in immunodiffusion serology. Research in Veterinary Science 40:157–160
    [Google Scholar]
  2. BANKS K. L., HENSON J. B., McGUIRE T. C. 1972; Immunologically mediated glomerulitis of horses. Laboratory Investigation 26:701–707
    [Google Scholar]
  3. BANKS K. L., CRAWFORD T. B., McGULRE T. C. 1978 Cellular immune mechanisms in the immunopathogenesis of equine infectious anemia. Equine Infectious Diseases IV359–362 Edited by Bryans J. T., Gerber H. New Jersey: Veterinary Publications;
    [Google Scholar]
  4. BARIN F., McLane M. F., ALLAN J. S., LEE T. H., GROOPMAN J. E., ESSEX M. 1985; Virus envelope protein of HTLV-III represents major target antigen for antibodies in AIDS patients. Science 228:1094–1096
    [Google Scholar]
  5. BRYANS J. T. 1969; On immunity to disease caused by equine herpesvirus 1. Journal of the American Veterinary Medical Association 155:294–300
    [Google Scholar]
  6. BURROWS R., GOODRIDGE D. 1973 In vivo and in vitro studies of equine rhmopneumonitis virus strains. Proceedings of the Third International Conference on Equine Infectious Diseases306–321 Edited by Bryans J. T., Gerber H. Basel: S. Karger;
    [Google Scholar]
  7. CHARMAN H. P., BLADEN S., GILDEN R. V., COGGINS L. 1976; Equine infectious anemia virus: evidence favoring classification as a retrovirus. Journal of Virology 19:1073–1079
    [Google Scholar]
  8. CHEEVERS W. P., ACKLEY C. M., CRAWFORD T. B. 1978; Structural proteins of equine infectious anemia virus. Journal of Virology 28:997–1001
    [Google Scholar]
  9. CHEEVERS W. P., ROBERTSON G. A., ARCHER B. G., CRAWFORD T. B. 1980; Orientation of structural polypeptides of equine infectious anemia virus. Intervirology 14:44–49
    [Google Scholar]
  10. CHIU I., YANIV A., DAHLBERG J. E., GAZIT A., SKUNTZ S. F., TRONICK S. R., AARONSON S. A. 1985; Nucleotide sequence evidence for relationship of AIDS retrovirus to lentiviruses. Nature, London 317:366–368
    [Google Scholar]
  11. CLAVEL F., KLATZMANN D., MONTAGNIER L. 1985; Deficient LAV, neutralizing capacity of sera from patients with AIDS or related syndromes. Lancet i:879–880
    [Google Scholar]
  12. COFFIN J., HAASE A., LEVY J. A., MONTAGNIER L., OROSZLAN S., TEICH N., TEMIN H., TOYOSHIMA K., VARMUS H., VOGT P., WEISS R. 1986; Human immunodeficiency viruses. Science 232:697–698
    [Google Scholar]
  13. COGGINS L. 1984; Carriers of equine infectious anemia virus. Journal of the American Veterinary Medical Association 184:279–281
    [Google Scholar]
  14. CRAWFORD T. B., McGUIRE T. C., HENSON J. B. 1971; Detection of equine infectious anemia virus in vitro by immunofluorescence. Archiv für die gesamte Virusforschung 34:332–339
    [Google Scholar]
  15. HENSON J. B., McGULRE T. C. 1971; Immunopathology of equine infectious anemia. American Journal of Clinical Pathology 56:306–314
    [Google Scholar]
  16. HO D. D., SARNGADHARAN M. G., HIRSCH M. S., SCHOOLEY R. T., ROTA T. R., KENNEDY R. C, CHANH T. C, SATO V. L. 1987; Human immunodeficiency virus neutralizing antibodies recognize several conserved domains on the envelope glycoproteins. Journal of Virology 61:2024–2028
    [Google Scholar]
  17. JOHNSON G. C, BARBET A. F., KLEVJER-ANDERSON P., McGULRE T. C. 1983; Preferential immune response to virion surface glycoproteins by caprine arthritis-encephalitis virus-infected goats. Infection and Immunity 41:657–665
    [Google Scholar]
  18. KLEVJER-ANDERSON P., McGUIRE T. C. 1982; Neutralizing antibody response of rabbits and goats to caprine arthritis-encephalitis virus. Infection and Immunity 38:455–461
    [Google Scholar]
  19. KONO Y. 1969; Viremia and immunological responses in horses infected with equine infectious anemia virus. National Institute of Animal Health Quarterly 9:1–9
    [Google Scholar]
  20. KONO Y., KOBAYASHI K., FUKUNAGA Y. 1973; Antigenic drift of equine infectious anemia virus in chronically infected horses. Archiv für die gesamte Virusforschung 41:1–40
    [Google Scholar]
  21. McGUIRE T. C., CRAWFORD T. B. 1979; Immunology of a persistent retrovirus infection - equine infectious anemia. Advances in Veterinary Science and Comparative Medicine 23:137–159
    [Google Scholar]
  22. McGUIRE T. C, HENSON J. B., QUIST S. E. 1969; Impaired bone marrow response in equine infectious anemia. American Journal of Veterinary Research 30:2099–2104
    [Google Scholar]
  23. McGUIRE T. C, CRAWFORD T. B., HENSON J. B. 1972; Equine infectious anemia: detection of infectious virus-antibody complexes in the serum. Immunological Communications 1:545–551
    [Google Scholar]
  24. MALMQUIST W. A., BARNETT D., BECVAR C. S. 1973; Production of equine infectious anemia antigen in a persistently infected cell line. Archiv für die gesamte Virusforschung 42:361–370
    [Google Scholar]
  25. MONTELARO R. C, LOHREY N., PAREKH B., BLAKENEY E. W., ISSEL C. J. 1982; Isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus. Journal of Virology 42:1029–1038
    [Google Scholar]
  26. MONTELARO R. C, PAREKH B., ORREGO A., ISSEL C. J. 1984; Antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus. Journal of Biological Chemistry 259:10539–10544
    [Google Scholar]
  27. MUMFORD J. A., BATES J. 1984; Trials of an inactivated equid herpesvirus 1 vaccine: challenge with a subtype 2 virus. Veterinary Record 114:375–381
    [Google Scholar]
  28. NARAYAN O., GRIFFIN D. E., CLEMENTS J. E. 1978; Virus mutation during ‘slow infection’; temporal development and characterization of mutants of visna virus recovered from sheep. Journal of General Virology 41:343–352
    [Google Scholar]
  29. NARAYAN O., CLEMENTS J. E., GRIFFIN D. E., WOLINSKY J. S. 1981; Neutralizing antibody spectrum determines the antigenic profiles of emerging mutants of visna virus. Injection and Immunity 32:1045–1050
    [Google Scholar]
  30. NARAYAN O., SHEFFER D., GRIFFIN D. E., CLEMENTS J., HESS J. 1984; Lack of neutralizing antibodies to caprine arthritis-encephalitis lentivirus in persistently infected goats can be overcome by immunization with inactivated Mycobacterium tuberculosis. Journal of Virology 49:349–355
    [Google Scholar]
  31. NISHIMURA M., NAKAJIMA H. 1984; Structural proteins of equine infectious anemia virus and their antigenic activity. American Journal of Veterinary Research 45:5–10
    [Google Scholar]
  32. PAREKH B., ISSEL C. J., MONTELARO R. C. 1980; Equine infectious anemia virus, a putative lentivirus, contains polypeptides analogous to prototype-C oncornaviruses. Virology 107:520–525
    [Google Scholar]
  33. PAYNE S. L., SALINOVICH O., NAUMAN S. M., ISSEL C. J., MONTELARO R. C. 1987; Course and extent of variation of equine infectious anemia virus during parallel persistent infections. Journal of Virology 61:1266–1270
    [Google Scholar]
  34. REED L. J., MUENCH H. 1938; A simple method of estimating fifty per cent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  35. SCHAFER W., BOLOGNESI D. P. 1977; Mammalian C-type oncornaviruses: relationship between viral structural and cell-surface antigens and their possible significance in immunological defense mechanisms. Contemporary Topics in Immunobiology 6:127–167
    [Google Scholar]
  36. SCOTT J. V., STOWRING L., HAASE A. T., NARAYAN O., VIGNE R. 1979; Antigenic variation in visna virus. Cell 18:321–327
    [Google Scholar]
  37. STEPHENS R. M., CASEY J. W., RICE N. R. 1986; Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus. Science 231:589–594
    [Google Scholar]
  38. THORMAR H., BARSHATZKY M. R., ARNESEN K., KOZLOWSKI P. B. 1983; The emergence of antigenic variants is a rare event in long-term visna virus infection in vivo. Journal of General Virology 64:1427–1432
    [Google Scholar]
  39. WEBER J. N., WEISS R. A., ROBERTS C, WELLER I., TEDDER R. S., CLAPHAM P. R., PARKER D., DUNCAN J., CARNE C, PINCHING A. J., CHEINGSONG-POPOV R. 1987; Human immunodeficiency virus infection in two cohorts of homosexual men: neutralizing sera and association of anti-gag antibody with prognosis. Lancet i:119–122
    [Google Scholar]
  40. WEISS R. A., CLAPHAM P., CHEINGSONG-POPOV R., DALGLEISH A. G., CARNE C. A., WELLER I. V. D., TEDDER R. S. 1985; Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients. Nature, London 316:69–71
    [Google Scholar]
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