1887

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Volume 69, Issue 3

Evolution of Avian Coronavirus IBV: Sequence of the Matrix Glycoprotein Gene and Intergenic Region of Several Serotypes Free

Abstract

Summary

We have sequenced 200 to 240 bases of the matrix (M) glycoprotein gene of 23 strains of infectious bronchitis virus (IBV) representing the A (D207), B (D3896), C (D3128), D (D212), Massachusetts (Mass), UK11 and UK12 serotypes. The bases examined code for the external, hydrophilic region and the first membrane-embedded hydrophobic region of M, both regions comprising approximately 20 amino acids. As predicted from protein studies the A/D and B/C serotypes had two and one potential glycosylation sites respectively. This variation appeared to derive from a combination of base substitutions and deletions/insertions. The glycosylation sequence Asn-Cys-Thr was highly conserved. Overall, the exposed part of M exhibited a fourfold greater extent of amino acid variation than did the membrane-embedded sequence. The transcription-associated homology region sequence (CUUAACAA) in the 5′ intergenic region was identical in all strains but there was considerable variation as to its location. The M gene of UK12 appeared to have evolved from a group A-like M gene by a two stage process involving a base substitution in the intergenic region which generated a new AUG translation start codon followed by deletion of the original AUG. Isolate UK11 closely resembled Mass strains in the intergenic region but was dissimilar from all strains in the protein coding region. The M sequences of serotypes B and C were identical and those of the A and D serotypes very similar. These results are discussed in relation to recent sequencing of part of the spike glycoprotein gene of some of these strains and the discovery of recombination of murine hepatitis coronavirus.

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  • Accepted:
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Keyword(s): evolution , infectious bronchitis virus and nucleotide sequence
© Society for General Microbiology 1988
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1988-03-01
2024-04-19
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References

  1. ALEXANDER D. J., GOUGH R. E. 1978; A long-term study of the pathogenesis of infection of fowls with three strains of avian infectious bronchitis virus. Research in Veterinary Science 24:228–233
     [Google Scholar]
  2. ARMSTRONG J., NIEMANN H., SMEEKENS S., ROTTIER P., WARREN G. 1984; Sequence and topology of a model intracellular membrane protein, E1 glycoprotein, from a coronavirus. Nature, London 308:751–752
     [Google Scholar]
  3. BINNS M. M., BOURSNELL M. E. G., TOMLEY F. M., BROWN T. D. K. 1986a; Nucleotide sequence encoding the membrane protein of the IBV strain 6/82. Nucleic Acids Research 14:5558
     [Google Scholar]
  4. BINNS M. M., BOURSNELL M. M. G., TOMLEY F. M., BROWN T. D. K. 1986b; Comparison of the spike precursor sequences of coronavirus IBV strains M41 and 6/82 with that of IBV Beaudette. Journal of General Virology 67:2825–2831
     [Google Scholar]
  5. BOURSNELL M. E. G., BROWN T. D. K., BINNS M. M. 1984; Sequence of the membrane protein gene from avian coronavirus IBV. Virus Research 1:303–313
     [Google Scholar]
  6. BOURSNELL M. E. G., BINNS M. M., BROWN T. D. K. 1985; Sequencing of coronavirus IBV genomic RNA: three open reading frames in the 5′ ‘unique’ region of mRNA D. Journal of General Virology 66:2253–2258
     [Google Scholar]
  7. BROWN T. D. K., BOURSNELL M. E. G. 1984; Avian infectious bronchitis virus genomic RNA contains sequence homologies at the intergenic boundaries. Virus Research 1:15–24
     [Google Scholar]
  8. BROWN T. D. K., BOURSNELL M. E. G., BINNS M. M. 1984; A leader sequence is present on mRNA A of avian infectious bronchitis virus. Journal of General Virology 65:1437–1442
     [Google Scholar]
  9. CAVANAGH D. 1983a; Coronavirus IBV glycopolypeptides: size of their polypeptide moieties and nature of their oligosaccharides. Journal of General Virology 64:1187–1191
     [Google Scholar]
  10. CAVANAGH D. 1983b; Coronavirus IBV: further evidence that the surface projections are associated with two glycopolypeptides. Journal of General Virology 64:1787–1791
     [Google Scholar]
  11. CAVANAGH D., DAVIS P. J. 1987; Coronavirus IBV: relationships among recent European isolates studied by limited proteolysis of the virion glycopolypeptides. Avian Pathology 16:1–13
     [Google Scholar]
  12. CAVANAGH D., DARBYSHIRE J. H., DAVIS P., PETERS R. W. 1984; Induction of humoral neutralizing and haemagglutination-inhibiting antibody by the spike protein of avian infectious bronchitis virus. Avian Pathology 13:573–583
     [Google Scholar]
  13. CAVANAGH D., DAVIS P. J., PAPPIN D. J. C. 1986a; Coronavirus IBV glycopolypeptides: locational studies using proteases and saponin, a membrane permeabilizer. Virus Research 4:145–156
     [Google Scholar]
  14. CAVANAGH D., DAVIS P. J., DARBYSHIRE I. H., PETERS R. W. 1986b; Coronavirus IBV: virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virus-neutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection. Journal of General Virology 67:1435–1442
     [Google Scholar]
  15. COLLINS A. R., KNOBLER R. L., POWELL H., BUCHMEIER M. J. 1982; Monoclonal antibodies to murine hepatitis virus (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. Virology 119:358–371
     [Google Scholar]
  16. COOK J. K. A. 1968; Duration of experimental infectious bronchitis in chickens. Research in Veterinary Science 9:506–514
     [Google Scholar]
  17. COOK J. K. A. 1984; The classification of new serotypes of infectious bronchitis virus isolated from poultry flocks in Britain between 1981 and 1983. Avian Pathology 13:733–741
     [Google Scholar]
  18. COOK J. K. A., HUGGINS M. B. 1986; Newly isolated serotypes of infectious bronchitis virus: their role in disease. Avian Pathology 15:129–138
     [Google Scholar]
  19. DAVELAAR F. G., KOUWENHOVEN B., BURGER A. G. 1983; Experience with vaccination against infectious bronchitis in broilers and significance of the vaccination against variant infectious bronchitis viruses in breeders and layers in The Netherlands. Clinica Veterinaria 106:7–11
     [Google Scholar]
  20. HASONY H. J., MacNAUGHTON M. R. 1981; Antigenicity of mouse hepatitis virus strain 3 subcomponents in C57 strain mice. Archives of Virology 69:33–41
     [Google Scholar]
  21. KECK J. G., STOHLMAN S. A., SOE L. H., MAKINO S., LAI M. M. C. 1987; Multiple recombination sites at the 5′-end of murine coronavirus RNA. Virology 156:331–341
     [Google Scholar]
  22. KÜSTERS J. G., MESTERS H. G. M., BLEUMINK-PLUYM N. M. C, DAVELAAR F. G., HORZINEK M. C., VAN DER ZEIIST B. A. M. 1987; Molecular epidemiology of infectious bronchitis virus in The Netherlands. Journal of General Virology 68:343–352
     [Google Scholar]
  23. LAI M. M. C, PATTON C. D., BARIC R. S., STOHLMAN S. A. 1983; Presence of leader sequences in the mRNA of mouse hepatitis virus. Journal of Virology 46:1027–1033
     [Google Scholar]
  24. LAPPS W., HOGUE B. G., BRIAN D. A. 1987; Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes. Virology 157:47–57
     [Google Scholar]
  25. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  26. MOCKETT A. P. A., CAVANAGH D., BROWN T. D. K. 1984; Monoclonal antibodies to the S1 spike and membrane proteins of avian infectious bronchitis coronavirus strain Massachusetts M41. Journal of General Virology 65:2281–2286
     [Google Scholar]
  27. NIESTERS H. G. M. 1987 Molecular epidemiology of infectious bronchitis virus Ph.D. thesis, University of Utrecht
    [Google Scholar]
  28. PFLEIDERER M., SKINNER M. A., SIDDELL S. G. 1986; Coronavirus MHV-JHM: nucleotide sequence of the mRNA that encodes the membrane protein. Nucleic Acids Research 14:6338
     [Google Scholar]
  29. ROTTIER P. J. M., WELLING G. W., WELLING-WESTER S., NIESTERS H. G. M., LENSTRA J. A., VAN DER ZEUST B. A. M. 1986; Predicted membrane topology of the coronavirus protein El. Biochemistry 25:1335–1339
     [Google Scholar]
  30. WOODS R. D., WESLEY R. D. 1987 Complement dependent virus neutralization of TGEV by anti-matrix monoclonal antibodies. Advances in Experimental Medicine and Biology Edited by Lai M. M. C., Stohlman S. A. London: Plenum Press; in press
    [Google Scholar]
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Evolution of Avian Coronavirus IBV: Sequence of the Matrix Glycoprotein Gene and Intergenic Region of Several Serotypes
J. Gen. Virol. 69, 621 (1988); https://doi.org/10.1099/0022-1317-69-3-621
/content/journal/jgv/10.1099/0022-1317-69-3-621
/content/journal/jgv/10.1099/0022-1317-69-3-621
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