An Antigenic Analysis of the Adenovirus Type 2 Fibre Polypeptide Free

Abstract

Summary

Twenty-seven monoclonal antisera were generated against the SDS-denatured fibre of adenovirus type 2. The antisera were characterized using radioimmune assay, fluorescent antibody tests, immune precipitation, Western blotting, haemagglutination and neutralization, and formed six groups as follows: A, type-specific neutralizing antisera which exhibited haemagglutination inhibition (Hi); B, type-specific non-neutralizing antisera which did not exhibit haemagglutination inhibition (Hi); C, subgroup-specific neutralizing antisera Hi; D, a subgroup-specific neutralizing antiserum Hi; E, subgroup-specific non-neutralizing antisera Hi; F, a subgroup-specific neutralizing antiserum Hi which did not react in Western blotting tests. The C-terminal 201 amino acids of the fibre were expressed in and a total of six antisera from groups A, B and C recognized five epitopes carried on this region which in several models is thought to form the knob of the fibre. At least eight epitopes were expressed by the entire native fibre. The five epitopes of the C-terminal end of the fibre formed three antigenic sites. Two sites each consisted of a neutralizing type-specific and a neutralizing group-specific epitope which overlapped in position. The remaining site consisted of a type-specific, non-neutralizing epitope.

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1988-03-01
2024-03-28
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References

  1. BANKS P. A., KASEL J. A., HUBER M., ALFORD R. H., KNIGHT V. 1966; Persistence of neutralizing antibody in adult volunteers immunized with adeno-virus soluble antigens. Proceedings of the Society for Experimental Biology and Medicine 121:240–243
    [Google Scholar]
  2. BIRNBOIM H. C., DOLY J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. BOUDIN M. L., BOULANGER P. 1981; Antibody-triggered dissociation of adenovirus penton. Virology 113:781–786
    [Google Scholar]
  4. DORSETT P. H., GINSBERG H. S. 1975; Characterisation of type 5 adenovirus fibre protein. Journal of Virology 15:208–216
    [Google Scholar]
  5. DRETZEN G., BALLARD M., SASSONE-CORRI P., CHAMBON P. 1981; A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Analytical Biochemistry 112:295–298
    [Google Scholar]
  6. FAZEKAS DE, ST. GROTH A., SCHIEDEGGER D. 1980; Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35:1–21
    [Google Scholar]
  7. GINSBERG H. 1979 Adenovirus structural proteins. Comparative Virology B409–427 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  8. GREEN N. M., WINGLEY N. G., RUSSELL W. C, MARTIN S. R., McLACHLAN A. D. 1983; Evidence for a repeating cross-β sheet structure in the adenovirus fibre. EMBO Journal 2:1357–1365
    [Google Scholar]
  9. HANAHAN D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  10. HAYASHI K., RUSSELL W. C. 1968; A study of the development of adenovirus antigens by the immunofluorescent technique. Virology 34:470–482
    [Google Scholar]
  11. HERISSE J., GALIBERT F. 1981; Nucleotide sequence of the EcoRI-E fragment of adenovirus 2 genome. Nucleic Acids Research 9:1229–1240
    [Google Scholar]
  12. HERISSE J., RIGOLET M., DUPONT DE DIENCHLN S., GALIBERT F. 1981; Nucleotide sequence of adenovirus 2 DNA fragment encoding for the carboxylic region of the fibre protein and the entire E4 region. Nucleic Acids Research 9:4023–4042
    [Google Scholar]
  13. HUNTER W. M. 1978 Radioimmunoassay. Immunochemistry 114.1–14.40 Edited by Weir D. M. Oxford: Blackwell Scientific;
    [Google Scholar]
  14. KJELLÉN L., PEREIRA H. G. 1968; Role of adenovirus antigens in the induction of virus neutralizing antibody. Journal of General Virology 2:177–185
    [Google Scholar]
  15. MESSING J. 1979 A multi-purpose cloning system based on the single stranded DNA bacteriophage M13. Recombinant DNA Technical Bulletin publication no. 79-99 243–48 Bethesda: NIH;
    [Google Scholar]
  16. MESSING J., CREA R., SEEBURG P. H. 1981; A system for shot-gun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  17. NORRBY E. 1966a; The relationship between the soluble antigens and the virion of adenovirus type 3. I. Morphological characteristics. Virology 28:236–248
    [Google Scholar]
  18. NORRBY E. 1966b; The relationship between the soluble antigens and the virion of type 3. II. Identification and characterisation of an incomplete hemagglutinin. Virology 30:608–617
    [Google Scholar]
  19. NORRBY E. 1968; Biological significance of structural adenovirus components. Current Topics in Microbiology and Immunology 43:1–43
    [Google Scholar]
  20. NORRBY E. 1969a; The relationship between the soluble antigens and the virion of adenovirus type 3. IV. Immunological complexity of soluble components. Virology 37:565–576
    [Google Scholar]
  21. NORRBY E. 1969b; The structural and functional diversity of adenovirus capsid components. Journal of General Virology 5:221–236
    [Google Scholar]
  22. NORRBY E., SKAARET P. 1967; The relationship between the soluble antigens and the virion of adenovirus type 3. III. Immunological identification of fibre antigen and isolated vertex capsomer antigen. Virology 32:489–502
    [Google Scholar]
  23. PEREIRA H. G., DE FIGUEIREDO M. V. T. 1962; Mechanism of hemagglutination by adenovirus types 1,2,4,5 and 6. Virology 18:1–8
    [Google Scholar]
  24. PETTERSSON U., HOGLUND S. 1969; Structural proteins and adenovirus type 2 penton antigen. Virology 39:90–106
    [Google Scholar]
  25. PETTERSSON U., PHILIPSON L., HOGLUND S. 1968; Structural proteins of adenoviruses. II. Purification and characterisation of adenovirus type 2 fibre antigen. Virology 35:204–215
    [Google Scholar]
  26. RUSSELL W. C., BLAIR G. E. 1977; Polypeptide phosphorylation in adenovirus-infected cells. Journal of General Virology 34:19–35
    [Google Scholar]
  27. RUSSELL W. C, HAYASHI K., SANDERSON P. J., PEREIRA H. G. 1967; Adenovirus antigens - a study of their properties and sequential development in infection. Journal of General Virology 1:495–507
    [Google Scholar]
  28. RUSSELL W. C, PATEL G., PRECIOUS B., SHARP I., GARDNER P. S. 1981; Monoclonal antibodies against adenovirus type 5: preparation and preliminary characterization. Journal of General Virology 56:393–408
    [Google Scholar]
  29. SIGNAS C, AKUSJARVI G., PETTERSSON U. 1985; Adenovirus 3 fiber polypeptide gene: implications for the structure of the fiber protein. Journal of Virology 53:672–678
    [Google Scholar]
  30. SUNDQUIST B., PETTERSSON U., THELANDER L., PHILIPSON L. 1973; Molecular weight and subunit composition of adenovirus type 2 fibre. Virology 51:252–256
    [Google Scholar]
  31. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  32. VALENTINE R. C., PEREIRA H. G. 1965; Antigens and structure of the adenovirus. Journal of Molecular Biology 13:13–20
    [Google Scholar]
  33. VAN OOSTRUM J., BURNETT R. M. 1985; Molecular composition of the adenovirus type 2 virion. Journal of Virology 56:439–448
    [Google Scholar]
  34. VIERA J., MESSING S. 1982; The pUC plasmids, an M13mp7 derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  35. WADELL C., NORRBY E. 1969; Immunological and other biological characteristics of pentons of human adenoviruses. Journal of Virology 4:671–680
    [Google Scholar]
  36. WILCOX W. C., GINSBERG H. S. 1963; Production of specific neutralizing antibody with soluble antigens of type 5 adenovirus. Proceedings of the Society for Experimental Biology and Medicine 114:37–42
    [Google Scholar]
  37. WINTERS W. D., RUSSELL W. C. 1971; Studies on the assembly of adenoviruses in vitro. Journal of General Virology 10:181–194
    [Google Scholar]
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