%0 Journal Article %A Darling, Allan J. %T The Principal Hydrogen Donor for the Herpes Simplex Virus Type 1-encoded Ribonucleotide Reductase in Infected Cells Is a Cellular Thioredoxin %D 1988 %J Journal of General Virology, %V 69 %N 3 %P 515-523 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-69-3-515 %K ribonucleotide reductase %K HSV-1 %K thioredoxin %I Microbiology Society, %X Summary In this study herpes simplex virus type 1-encoded ribonucleotide reductase was shown to be able to utilize thioredoxin purified from the cyanobacterium Anabaena variabilis as a hydrogen donor for the enzyme. An assay has been developed to search for proteins which can function as a hydrogen donor for the viral ribonucleotide reductase. A protein has been identified and purified to homogeneity from infected cell extracts by a combination of fast protein liquid chromatography and gel filtration. This protein, which is also present in mock-infected cells, has been identified as a host cell thioredoxin by similarities in its physical characteristics with other thioredoxins. No evidence for the existence of a major virus-induced thioredoxin was obtained, suggesting that the host cell thioredoxin functions as the hydrogen donor for the herpes simplex virus type 1 ribonucleotide reductase in the infected cell. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-69-3-515