1887

Abstract

Summary

Comparison by HPLC of tryptic digests of coat proteins from six biologically and serologically distinct potyviruses, namely bean yellow mosaic virus, Johnson grass mosaic virus, passion-fruit woodiness virus (PWV), potato virus Y (PVY), sugarcane mosaic virus (SCMV) and water-melon mosaic virus II, demonstrated that each potyvirus can be distinguished from the others. HPLC of tryptic peptides from coat proteins of four strains of PVY, two strains of PWV and three strains of SCMV showed that peptide patterns of strains from the same potyvirus were very similar. These findings were supported by amino-terminal amino acid sequence analysis of the peptides. The use of enzymes from different sources and variation in the temperature (35 °C to 40 °C) and time (16 to 20 h) of digestion caused small variations in the profiles but did not change the main features of the peptide patterns of each potyvirus. The results suggest that HPLC profiles of tryptic digests of the coat proteins of potyviruses could be useful criteria for the identification and classification of potyviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-3-493
1988-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/3/JV0690030493.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-3-493&mimeType=html&fmt=ahah

References

  1. ABU-SAMAH N., RANDLES J. W. 1981; A comparison of nucleotide sequence homologies of three isolates of bean yellow mosaic virus and their relationship to other potyviruses. Virology 110:436–444
    [Google Scholar]
  2. ALLISON R. F., DOUGHERTY W. G., PARKS T. D., WILLIS L., JOHNSTON R. E., KELLY M. E., ARMSTRONG F. B. 1985a; Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located at the virion’s surface. Virology 147:309–316
    [Google Scholar]
  3. ALLISON R. F., SORENSON J. C, KELLY M. E., ARMSTRONG F. B., DOUGHERTY W. G. 1985b; Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: evidence for the synthesis and processing of a polyprotein in potyvirus genome expression. Proceedings of the National Academy of Sciences, U.S.A 82:3969–3972
    [Google Scholar]
  4. BOS L. 1970; Bean yellow mosaic virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses40
    [Google Scholar]
  5. DOMIER L. L., FRANKLIN K. M., SHAHABUDDIN M., HELMAN G. M., OVERMEYER J. H., HIREMATH S. T., SIAW M. F. E., LOMONOSSOFF G. P., SHAW J. G., RHOADS E. 1986; The nucleotide sequence of tobacco vein mottling virus. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  6. DOUGHERTY W. G., ALLISON R. F., PARKS T. D., JOHNSTON R. E., FEILD M. J., ARMSTRONG F. B. 1985; Nucleotide sequence at the 3′ terminus of pepper mottle virus genomic RNA: evidence for an alternative mode of potyvirus capsid protein organization. Virology 146:282–291
    [Google Scholar]
  7. EDWARDSON J. R. 1974; Some properties of the potato virus Y-group. Monograph Series, Florida Agricultural Experimental Station 4:85
    [Google Scholar]
  8. GOUGH K. H., SHUKLA D. D. 1981; Coat protein of potyviruses. 1. Comparison of the four Australian strains of sugarcane mosaic virus. Virology 111:455–462
    [Google Scholar]
  9. GOUGH K. H., AZAD A. A., HANNA P. J., SHUKLA D. D. 1987; Nucleotide sequence of the capsid and nuclear inclusion protein genes from the Johnson grass strain of sugarcane mosaic virus RNA. Journal of General Virology 68:297–304
    [Google Scholar]
  10. GREBER R. S. 1978; Watermelon mosaic virus 1 and 2 in Queensland cucurbit crops. Australian Journal of Agricultural Research 29:1235–1245
    [Google Scholar]
  11. HEWISH D. R., SHUKLA D. D., GOUGH K. H. 1986; The use of biotin conjugated antisera in immunoassays for plant viruses. Journal of Virological Methods 13:79–85
    [Google Scholar]
  12. HOLLINGS M., BRUNT A. A. 1981a Potyviruses. Handbook of Plant Virus Infections and Comparative Diagnosis731–807 Edited by Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  13. HOLLINGS M., BRUNT A. A. 1981b; Potyvirus group. Commonwealth Mycological Institute)’Association of Applied Biologists Descriptions of Plant Viruses245
    [Google Scholar]
  14. JONES R. T., DIACHUN S. 1977; Serologically and biologically distinct bean yellow mosaic virus strains. Phytopathology 67:831–838
    [Google Scholar]
  15. MEEK J. L. 1980; Prediction of peptide retention times in high-pressure liquid chromatography on the basis of amino acid composition. Proceedings of the National Academy of Sciences, U.S.A 77:1632–1636
    [Google Scholar]
  16. MOGHAL S. M., FRANCKI R. I. B. 1976; Towards a system for the identification and classification of potyviruses. 1. Serology and amino acid composition of six distinct viruses. Virology 73:350–362
    [Google Scholar]
  17. O’HARE M. J., NICE E. C. 1979; Hydrophobic high-performance liquid chromatography of hormonal polypeptides and proteins on alkylsilane-bonded silica. Journal of Chromatography 171:209–226
    [Google Scholar]
  18. RANDLES J. W., DAVIES C, GIBBS A. J., HATTA T. 1980; Amino acid composition of capsid protein as a taxonomie criterion for classifying the atypical S strain of bean yellow mosaic virus. Australian Journal of Biological Sciences 33:245–254
    [Google Scholar]
  19. REDDICK B. B., BARNETT O. W. 1983; A comparison of three potyviruses by direct hybridization analysis. Phytopathology 73:1506–1510
    [Google Scholar]
  20. SHUKLA D. D., GOUGH K. H. 1984; Serological relationships among four Australian strains of surgarcane mosaic virus as determined by immune electron microscopy. Plant Disease 68:204–206
    [Google Scholar]
  21. SHUKLA D. D., O’DONNELL I. I., GOUGH K. H. 1983; Characteristics of the electro-blot radioimmunoassay (EBRIA) in relation to the identification of plant viruses. Acta Phytopathologica Academiae Scientiarum Hungaricae 18:79–84
    [Google Scholar]
  22. SHUKLA D. D, INGLIS A. S., McKern N. M., GOUGH K. H. 1986; Coat protein of potyviruses. 2. amino acid sequence of the coat protein of potato virus Y. Virology 152:118–125
    [Google Scholar]
  23. SHUKLA D. D., GOUGH K. H., WARD C. W. 1987; Coat protein of potyviruses. 3. Comparison of amino acid sequences of the coat proteins of four Australian strains of sugarcane mosaic virus. Archives of Virology 96:59–74
    [Google Scholar]
  24. TEAKLE D. S., GRYLLS N. E. 1973; Four strains of sugarcane mosaic virus infecting cereals and other grasses in Australia. Australian Journal of Agricultural Research 24:465–477
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-3-493
Loading
/content/journal/jgv/10.1099/0022-1317-69-3-493
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error