1887

Abstract

Summary

Barley tissue with an acute systemic infection of barley stripe mosaic virus contained a large amount of unencapsidated virus RNA which was stable in extracts made in ribosome isolation buffer. The virus RNA in ribosome preparations sedimented in a broad band at 80S to 100S in sucrose gradients, which is less than the virion sedimentation rate of 180S to 200S. A protein of apparent 60000, which sedimented with the virus RNA, was present in ribosome extracts from infected plants but absent from those from uninfected plants. The protein is probably a virus protein because its apparent molecular weight varied slightly with the strain of virus. The structure containing the 60000 protein did not sediment in sucrose gradients in a compact zone as would be expected for a particle of uniform size. The 60000 protein was present at a concentration equal to or slightly higher (up to 400 µg/g leaf tissue) than the unencapsidated virus RNA (up to 300 µg/g leaf tissue). Sedimentation results support the conclusion that the virus RNA and the 60000 protein were combined in polydispersed nucleoprotein particles which may or may not have been attached to ribosomes or ribosome subunits. The 60000 protein was not serologically related to the capsid protein. Gold-labelled antibodies to the 60000 protein stained the cytoplasm in thin sections of infected cells but not that of uninfected cells. However, no distinct immunogold-labelled particle could be identified as the presumed nucleoprotein.

Keyword(s): BMSV , hordeivirus and unencapsidated RNA
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-3-481
1988-03-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/3/JV0690030481.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-3-481&mimeType=html&fmt=ahah

References

  1. ATABEKOV J. G., DOLJA V. V. 1986 Hordeiviruses, structure and replication. The Plant Viruses397–420 Edited by Van Regenmortel M. H. V., Fraenkel-Conrat H. New York & London: Plenum Press;
    [Google Scholar]
  2. ATABEKOV J. G., MOROZOV S. Y. 1979; Translation of plant virus messenger RNAs. Advances in Virus Research 25:1–91
    [Google Scholar]
  3. BRAKKE M. K. 1962; Stability of purified barley stripe mosaic virus. Virology 17:131–142
    [Google Scholar]
  4. BRAKKE M. K. 1963; Photometric scanning of centrifuged density gradient columns. Analytical Biochemistry 5:271–283
    [Google Scholar]
  5. BRAKKE M. K. 1972; Slowly sedimenting infectious entities of southern bean mosaic virus. Virology 50:669–680
    [Google Scholar]
  6. BRAKKE M. K., WHITE J. L., SAMSON R. G., JOSHI J. 1987; Chlorophyll, chloroplast ribosomal RNA and the amount of DNA per barley leaf are reduced by barley stripe mosaic virus systemic infection. Phytopathology in press
    [Google Scholar]
  7. DAVIES E., LARKINS B. A., KNIGHT R. H. 1972; Polyribosomes from peas. An improved method for their isolation in the absence of ribonuclease inhibitors. Plant Physiology 50:581–584
    [Google Scholar]
  8. DEAN C., LEECH R. M. 1982; Genome expression during normal leaf development. I. Cellular and chloroplast numbers and DNA, RNA and protein levels in tissues of different ages within a seven-day-old wheat leaf. Plant Physiology 69:904–910
    [Google Scholar]
  9. DIENER T. O. 1962; Isolation of an infectious, ribonuclease-sensitive fraction from tobacco leaves recently infected with tobacco mosaic virus. Virology 16:140–146
    [Google Scholar]
  10. DOLJA V. V., ATABEKOV J. G. 1987; The structure of barley stripe mosaic virus double-stranded RNAs. FEBS Letters 214:313–316
    [Google Scholar]
  11. DOLJA V. V., LUNINA N. A., LEISER R.-M., STANARIUS T., BELZHELARSKAYA S. N., KOZLOFF YU. V., ATABEKOV J. G. 1983; A comparative study on the in vitro translation products of individual RNAs from two-, three-, and four-component strains of barley stripe mosaic virus. Virology 127:1–14
    [Google Scholar]
  12. DOROKHOV Y. L., ALEXANDROVA N. M., MIROSCHNICHENKO N. A., ATABEKOV J. G. 1983; Isolation and analysis of virus-specific ribonucleoprotein of tobacco mosaic virus-infected tobacco. Virology 127:237–252
    [Google Scholar]
  13. DOROKHOV Y. L., ALEXANDROVA N. M., MIROSCHNICHENKO N. A., ATABEKOV J. G. 1984; The informosome-like virus-specific ribonucleoprotein (vRNP) may be involved in the transport of tobacco mosaic virus infection. Virology 137:127–134
    [Google Scholar]
  14. GUSTAFSON G. D., ARMOUR S. L. 1986; The complete nucleotide sequence of RNA beta from the type strain of barley stripe mosaic virus. Nucleic Acid Research 14:3895–3909
    [Google Scholar]
  15. GUSTAFSON G. D., LARKINS B. A., JACKSON A. O. 1981; Comparative analysis of polypeptides synthesized in vivo and in vitro by two strains of barley stripe mosaic virus. Virology 111:579–587
    [Google Scholar]
  16. GUSTAFSON G., HUNTER B., HANAU R., ARMOUR S. L., JACKSON A. O. 1987; Nucleotide sequence and genetic organization of barley stripe mosaic virus RNA gamma. Virology 158:394–406
    [Google Scholar]
  17. HSU Y.-H. 1984; Immunogold for detection of antigen on nitrocellulose paper. Analytical Biochemistry 142:221–225
    [Google Scholar]
  18. JACKSON A. O., BRAKKE M. K. 1973; Multicomponent properties of barley stripe mosaic virus ribonucleic acid. Virology 55:483–494
    [Google Scholar]
  19. JACKSON A. O., LANE L. C. 1981 Hordeiviruses. Handbook of Plant Virus Infections565–652 Edited by Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  20. JACKSON A. O., DAWSON J. R. O., COVEY S. N., HULL R., DAVIES J. W., McFarland J. E., GUSTAFSON G. D. 1983; Sequence relations and coding properties of a subgenomic RNA isolated from barley stripe mosaic virus. Virology 127:37–44
    [Google Scholar]
  21. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  22. LIN N.-S., LANGENBERG W. G. 1983; Immunohistochemical localization of barley stripe mosaic virions in infected cells. Journal of Ultrastructure Research 84:16–23
    [Google Scholar]
  23. McKINNEY H. H., GREELEY L. W. 1965; Biological characteristics of barley stripe mosaic virus strains and their evolution. Technical Bulletin. U.S. Department of Agriculture 1324:84
    [Google Scholar]
  24. MERRIL C. R., GOLDMAN D., SEDMAN S. A., EBERT M. H. 1981; Ultrasensitive stain for proteins in Polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437–1438
    [Google Scholar]
  25. PRING D. R. 1971; Viral and host RNA synthesis in BSMV-infected barley. Virology 44:54–66
    [Google Scholar]
  26. PRING D. R. 1972; Barley stripe mosaic virus replicative form RNA: preparation and characterization. Virology 41:22–29
    [Google Scholar]
  27. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  28. WHITE J. L., BRAKKE M. K. 1983; Protein changes in wheat infected with wheat streak mosaic virus and in barley infected with barley stripe mosaic virus. Physiological Plant Pathology 22:87–100
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-3-481
Loading
/content/journal/jgv/10.1099/0022-1317-69-3-481
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error