Human monocytes appear to be very important in the pathogenesis of dengue infection. They are thought to be the most active sites of virus replication during dengue infection. We have analysed interferon (IFN) production by dengue virus from peripheral blood mononuclear cells (PBMC). IFN activity was first detected at 12 h after infection of monocytes and reached a maximum level by 48 h. Non-adherent PBMC depleted of monocytes did not produce detectable levels of IFN, and did not contain dengue antigen-positive cells after exposure to dengue virus. The IFN produced was characterized as IFN-α by neutralization tests using specific antisera to HuIFN-α, HuIFN-β and HuIFN-γ, and by radioimmunoassay. The culture fluids of dengue virus-infected monocytes, which contained IFN-α, were able to inhibit infection of human monocytes by dengue virus. These results suggest that IFN-α produced by dengue virus-infected monocytes may play an important role in controlling primary dengue virus infection.
BOONPUCKNAVIGS., BOONPUCKNAVIGV., BHAMARAPRAVATIN., NIMMANITYAS.1979; Immunofluorescence study of skin rash in patients with dengue hemorrhagic fever. Archives of Pathology and Laboratory Medicine 103:463–466
BOONPUCKNAVIGV., BHAMARAPRAVATIN., BOONPUCKNAVIGS., FUTRAKULP., TANPAICHITRP.1976; Glomerular changes in dengue hemorrhagic fever. Archives of Pathology and Laboratory Medicine 100:206–212
BURKED. S., MORRILLJ. C.1987; Levels of interferon in the plasma and cerebrospinal fluid of patients with acute Japanese encephalitis. Journal of Infectious Diseases 155:797–799
ENNISF. A., MEAGERA.1981; Immune interferon produced to high levels by antigenic stimulation of human lymphocytes with influenza virus. Journal of Experimental Medicine 154:1279–1289
GRESSERI., TOVEYM. G., MAURYC., BANDUM. T.1976; Role of interferon in the pathogenesis of virus diseases in mice as demonstrated by the use of anti-interferon serum. II. Studies with herpes simplex, Moloney sarcoma, vesicular stomatitis, Newcastle disease, and influenza viruses. Journal of Experimental Medicine 144:1316–1323
HALSTEADS. B.1980b Immunological parameters of togavirus disease syndromes. The Togaviruses: Biology, Structure, Replication107–173 Edited by SchlesingerR. W. New York: Academic Press;
HALSTEADS. B., O’ROURKEE. J., ALLISONA. C.1977; Dengue viruses and mononuclear phagocytes. II. Identity of blood and tissue leukocytes supporting in vitro infection. Journal of Experimental Medicine 146:218–229
KURANEI., ENNISF. A.1987; Induction of interferon alpha from human lymphocytes by autologous, dengue virus-infected monocytes. Journal of Experimental Medicine 166:999–1010
KURANEI., MEAGERA., ENNISF. A.1986; Induction of interferon alpha and gamma from human lymphocytes by dengue virus-infected cells. Journal of General Virology 67:1653–1661
WHEELOCKE. F., SIBLEYW. A.1965; Circulating virus, interferon and antibody after vaccination with the 17-D strain of yellow-fever virus. New England Journal of Medicine 273:194–198