Attempts were made to label the termini of dsRNAs corresponding to the two genomic RNAs of grapevine chrome mosaic nepovirus (GCMV). It was not possible to label the 5′ ends of the dsRNAs with [γ-32P]ATP, which suggests that a genome-linked protein blocks their 5′ ends. Both dsRNA species were labelled at their 3′ ends with pCp. The 3′-terminal sequences were determined by ‘wandering spot’ or by partial enzymic cleavage analysis. One strand (presumably positive) ended in a poly(A) 30 to 50 nucleotides long whereas the other (presumably negative) ended in 3′-ACCUUUUAAAAAG (RNA1) or 3′-ACCUUUUAAUAAAG (RNA2). The sequences resemble closely those complementary to the 5′ ends of the RNAs of tomato black ring virus (strain S), which is distantly related to GCMV.
BRETOUTC.1987Contribution à l’étude du phénomène de la prémunition entre deux souches du virus des anneaux noirs de la tomate (TBRV) sur Chenopodium quinoa Wild Ph.D. thesis, Université de Bordeaux II
COLLMERC. W., KAPERJ. M.1985; Double-stranded RNAs of cucumber mosaic virus and its satellite contain an unpaired guanosine: implications for replication. Virology 145:249–259
DE WACHTERR., FIERSW.1982 Two-dimensional gel electrophoresis of nucleic acids. Gel Electrophoresis of Nucleic Acids: A Practical Approach77–116 Edited by RickwoodD., HamesB. D. Oxford: IRL Press;
DODDS. M., ROBINSOND. J.1984; Nucleotide sequence homologies among RNA species of strains of tomato black ring virus and other nepoviruses. Journal of General Virology 65:1731–1740
DOZB., MACQUAIREG., DELBOSR., DUNEZJ.1980; Caractéristiques et rôle du RNA3, RNA satellite du virus des anneaux noirs de la tomate. Annales de Virologie 131E:489–499
HEMMERO., MEYERM., GREIFC., FRITSCHC.1987; Comparison of the nucleotide sequences of five tomato black ring virus satellite RNAs. Journal of General Virology 68:1823–1833
KIKUCHIY., KAZIMIERZT., FLLIPOWICZW., SANGERH. L., GROSSH. J.1982; Circularization of linear viroid RNA via 2′-phosphomonoester, 3′,5′-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas. Nucleic Acids Research 10:7521–7529
MAYOM. A., BARKERH., HARRISONB. D.1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
MEYERM., HEMMERO., FRITSCHC. 1984; Complete nucleotide sequence of a satellite RNA of tomato black ring virus. Journal of General Virology 65:1575–1583