1887

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Volume 69, Issue 2

Biochemical and Biophysical Characteristics of Rio Bravo Virus () Free

Abstract

Summary

Rio Bravo (RB) virus has been assigned to the family on the basis of its antigenic relatedness to other members of this family. RB virus, unlike most members of the , is believed not to have an arthropod vector. We examined biochemical and biophysical characteristics of RB virus to determine whether it should be assigned to the and to compare it with arthropod-borne flaviviruses. Purified RB virus banded at a density of 1.18 g/ml in sucrose and had a sedimentation coefficient of about 200 S. Virions, negatively stained with ammonium molybdate, were spherical, had diameters of 42 nm, and appeared to be surrounded by envelopes bearing surface projections. The loss of infectivity after infectious virus was incubated with diethyl ether or sodium deoxycholate confirmed the presence of envelopes. Partially purified RB virions contained single-stranded RNA, lacking 3′ poly(A) tracts, that sedimented in a 15% to 30% sucrose gradient as one discrete band with a sedimentation coefficient of about 40 S. Most of the viral proteins in preparations of purified virus and in immunoprecipitates had similar electrophoretic mobilities and glycosylation patterns to known flavivirus proteins. Therefore, they were assigned the following tentative designations using the nomenclature for flavivirus proteins: gp52 and gp47, envelope proteins; gp46, non-structural protein 1;p25, gp20(prM), precursor to membrane protein; gp < 18K. Putative core and membrane proteins were not identified. These physical and biochemical characteristics of RB virus are remarkably similar to those of the arthropod-borne members of the and they confirm the classification of RB virus in this family. This is the first report of biochemical and physical properties of a non-arthropod-borne member of the .

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Keyword(s): biophysical characteristics , flavivirus , non-arthropod-borne and Rio Bravo virus
© Society for General Microbiology 1988
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1988-02-01
2024-04-19
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References

  1. ALLEN R., TAYLOR S. E., SULKIN S. E. 1970; Studies of arthropod-borne virus infections in Chiroptera. VII. Evidence of natural St. Louis encephalitis virus infection in bats. American Journal of Tropical Medicine and Hygiene 19:851–859
     [Google Scholar]
  2. AVIV H., LEDER P. 1972; Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proceedings of the National Academy of Sciences, U.S.A 69:1408–1412
     [Google Scholar]
  3. BAER G. M., WOODALL D. F. 1966; Bat salivary gland virus carrier state in a naturally infected Mexican freetail bat. American Journal of Tropical Medicine and Hygiene IS:769–771
     [Google Scholar]
  4. BERGE T. O. 1975 International Catalog of Arboviruses Including Certain Other Viruses of Vertebrates, 2nd edn. Washington: Department of Health, Education and Welfare; Publication Number 75-8301
    [Google Scholar]
  5. BOLOGNESI D. P., LANGLOIS A. J. 1975; Polypeptides of mammalian oncornaviruses. IV. Structural components of murine leukemia virus released as soluble antigens in cell culture. Virology 68:550–555
     [Google Scholar]
  6. BRAWERMAN G. 1974; The isolation of messenger RNA from mammalian cells. Methods in Enzymology 30:605–612
     [Google Scholar]
  7. BRAWERMAN G., MEDECKI J., LEE S. Y. 1972; A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry 11:637–641
     [Google Scholar]
  8. BRINTON M. A. 1986 Replication of flaviviruses. The Togaviridae and Flaviviridae343–373 Edited by Schlesinger S., Schlesinger M. J. New York: Plenum Press;
     [Google Scholar]
  9. BURNS K. F., FARINACCI C. J. 1956; Virus of bats antigenically related to St. Louis encephalitis. Science 123:227
     [Google Scholar]
  10. BURNS K. F., FARINACCI C. J., SHELTON D. F. 1957; Virus of bats antigenically related to group B arthropod-borne encephalitis viruses. American Journal of Clinical Pathology 27:257–264
     [Google Scholar]
  11. CAHOON B. E., HARDY J. L., REEVES W. C. 1978; Initiation and characterization of a diploid cell line from larval tissues of Aedes dorsalis (Meigen). In Vitro 14:255–260
     [Google Scholar]
  12. CALISHER C. H., DAVIS J., COLEMAN P. H., LORD R. D., WORK T. H. 1969; Cowbone Ridge virus, a new group B arbovirus from South Florida. American Journal of Epidemiology 89:211–216
     [Google Scholar]
  13. CHEN A. B., BEN-PORAT T., WHITLEY R. J., KAPLAN A. S. 1978; Purification and characterization of proteins excreted by cells infected with herpes simplex virus and their use in diagnosis. Virology 91:234–242
     [Google Scholar]
  14. CONSTANTINE D. G., WOODALL D. F. 1964; Latent infection of Rio Bravo virus in salivary glands of bats. Public Health Reports 79:1033–1039
     [Google Scholar]
  15. DELLA-PORTA A. J., WESTAWAY W. G. 1972; Rapid preparation of hemagglutinins of togaviruses from infected cell fluids. Applied Microbiology 23:158–160
     [Google Scholar]
  16. DE MADRID A. T., PORTERFIELD J. S. 1974; The flaviviruses (group B arboviruses): a cross-neutralization study. Journal of General Virology 23:91–96
     [Google Scholar]
  17. HEINZ F. X., KUNZ C. 1982; Molecular epidemiology of tick-borne encephalitis virus: peptide mapping of large non-structural proteins of European isolates and comparison with other flaviviruses. Journal of General Virology 62:271–285
     [Google Scholar]
  18. HENDRICKS D. A., HARDY J. L., REEVES W. C. 1983; Comparison of biological properties of St. Louis encephalitis and Rio Bravo viruses. American Journal of Tropical Medicine and Hygiene 32:602–609
     [Google Scholar]
  19. HENDRICKS D. A., BARADARAN K., McINTOSH E., PATTERSON J. L. 1987; Appearance of a soluble form of the G protein of respiratory syncytial virus in fluids of infected cells. Journal of General Virology 67:1705–1714
     [Google Scholar]
  20. HORZINEK M. C. 1981 Non-arthropod-borne Togaviruses New York: Academic Press;
     [Google Scholar]
  21. JOHNSON H. N. 1962; The Rio Bravo virus: virus identified with group B arthropod-borne viruses by hemagglutination inhibition and complement-fixation tests. Proceedings of the Ninth Pacific Science Congress 17:39
     [Google Scholar]
  22. KANG C. Y., PREVEC L. 1971; Proteins of vesicular stomatitis virus. III. Intracellular synthesis and extracellular appearance of virus-specific proteins. Virology 46:678–690
     [Google Scholar]
  23. KITANO T., SUZUKI K., YAMAGUCHI T. 1974; Morphological, chemical and biological characterization of Japanese encephalitis virus virion and its hemagglutinin. Journal of Virology 14:631–639
     [Google Scholar]
  24. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  25. LITTLE S. P., HUANG A. S. 1977; Synthesis and distribution of vesicular stomatitis virus-specific polypeptides in the absence of progeny production. Virology 81:37–47
     [Google Scholar]
  26. McEWEN C. R. 1967; Tables estimating sedimentation through linear concentration gradients of sucrose solution. Analytical Biochemistry 20:114–149
     [Google Scholar]
  27. MAIN O. M., HARDY J. L., REEVES W. C. 1977; Growth of arboviruses and other viruses in a continuous line of Culex tarsalis cells. Journal of Medical Entomology 14:107–112
     [Google Scholar]
  28. MURPHY F. A. 1980 Togavirus morphology and morphogenesis. The Togaviruses. Biology, Structure, Replication241–316 Edited by Schlesinger R. W. New York: Academic Press;
     [Google Scholar]
  29. PEAT A., BELL T. M. 1970; Entebbe bat salivary virus: electron microscopic study of morphology and development in new born mice. Archiv für die gesamte Virusforschung 31:230–236
     [Google Scholar]
  30. QURESHI A. A., TRENT D. W. 1973; Group B arbovirus structural and nonstructural antigens. II. Purification of St. Louis encephalitis virus intracellular antigens. Infection and Immunity 8:985–992
     [Google Scholar]
  31. RICE C. M., STRAUSS E. G., STRAUSS J. H. 1986 Structure of the flavivirus genome. The Togaviridae and Flaviviridae279–326 Edited by Schlesinger S., Schlesinger M. J. New York: Plenum Press;
     [Google Scholar]
  32. RUSSELL P. E., BRANDT W. B., DALRYMPLE J. M. 1980 Chemical and antigenic structure of flaviviruses. The Togaviruses. Biology, Structure, Replication503–529 Edited by Schlesinger R. W. New York: Academic Press;
     [Google Scholar]
  33. SCHLESINGER J. J., BRANDRISS M. W., MONATH T. P. 1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition, and immune precipitation of the virus envelope protein. Virology 125:8–17
     [Google Scholar]
  34. SHOPE R. E., SATHER G. E. 1979 Arboviruses. Diagnostic Procedure for Viral, Rickettsial and Chlamydial Infections, 5th edn.767–841 Edited by Lennette E. H., Schmidt N. J. Washington: American Public Health Association;
     [Google Scholar]
  35. SMITH G. W., WRIGHT P. J. 1985; Synthesis of proteins and glycoproteins in dengue type 2 virus-infected Vero and Aedes albopictus cells. Journal of General Virology 66:559–571
     [Google Scholar]
  36. SULKIN S. E. 1962; The bat as a reservoir of viruses in nature. Progress in Medical Virology 4:157–201
     [Google Scholar]
  37. SULKIN S. E., ALLEN R. 1974; Virus infection in bats. Monographs in Virology 8:66–67
     [Google Scholar]
  38. TRENT D. W. 1977; Antigenic characterization of flavivirus structural proteins separated by isoelectric focusing. Journal of Virology 22:608–618
     [Google Scholar]
  39. WESTAWAY E. G., REEDMAN B. M. 1969; Proteins of the group B arbovirus Kunjin. Journal of Virology 4:688–693
     [Google Scholar]
  40. WESTAWAY E. G., SCHLESINGER R. W., DALRYMPLE J. M., TRENT D. W. 1980; Nomenclature of flavivirus-specified proteins. Intervirology 14:114–117
     [Google Scholar]
  41. WESTAWAY E. G., BRINTON M. A., GAIDAMOVICH S. Y., HORZINEK M. C, IGARASHI A., KÄÄRIÄINEN L., LVOV D. K., PORTERFIELD J. S., RUSSELL P. K., TRENT D. W. 1985; Flaviviridae. Intervirology 24:183–192
     [Google Scholar]
  42. WHITMAN L. 1959 Personal communication to Clarke, D. H. and Casals, J. Viral and Rickettsial Infections of Man, 4th edn.646 Edited by Horsfall F. L., Tamm I. Philadelphia: Lippincott;
     [Google Scholar]
  43. WILHELM A. R., GERONE P. J. 1970; Growth of Rio Bravo virus in cell culture. Applied Microbiology 20:612–615
     [Google Scholar]
  44. WRIGHT P. J. 1982; Envelope protein of the flavivirus Kunjin is apparently not glycosylated. Journal of General Virology 59:29–38
     [Google Scholar]
  45. WRIGHT P. J., WARR H. M., WESTAWAY E. G. 1981; Synthesis of glycoproteins in cells infected with the flavivirus Kunjin. Virology 109:418–127
     [Google Scholar]
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Biochemical and Biophysical Characteristics of Rio Bravo Virus (Flaviviridae)
J. Gen. Virol. 69, 337 (1988); https://doi.org/10.1099/0022-1317-69-2-337
/content/journal/jgv/10.1099/0022-1317-69-2-337
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