@article{mbs:/content/journal/jgv/10.1099/0022-1317-69-2-293, author = "Routledge, E. G. and Willcocks, M. M. and Samson, A. C. R. and Morgan, L. and Scott, R. and Anderson, J. J. and Toms, G. L.", title = "The Purification of Four Respiratory Syncytial Virus Proteins and Their Evaluation as Protective Agents against Experimental Infection in BALB/c Mice", journal= "Journal of General Virology", year = "1988", volume = "69", number = "2", pages = "293-303", doi = "https://doi.org/10.1099/0022-1317-69-2-293", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-69-2-293", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "purification", keywords = "proteins", keywords = "RSV", keywords = "respiratory syncytial virus", abstract = "Summary The fusion (F) glycoprotein, large glyco- (G) protein, phospho- (P) protein and 22K protein of respiratory syncytial (RS) virus A2 strain were purified by a combination of immunoaffinity adsorption and preparative SDS-PAGE. All four proteins elicited serum antibody in mice after repeated inoculation in adjuvant, although the magnitude of the response as measured by ELISA varied from mouse to mouse. The F protein generated neutralizing antibodies in only 50% of the mice determined to be seropositive by ELISA. The G protein also induced neutralizing antibodies but in this instance neutralization tests and ELISA titres were more closely correlated. No neutralizing activity was detected in mice immunized with the P or 22K proteins although all produced antibody detectable by ELISA. Mice immunized with either the F or the G protein were found to be protected against subsequent RS virus challenge, whether they had developed neutralizing antibody or not. Mice inoculated with the P or 22K proteins were not protected.", }