Foot-and-mouth disease virus (FMDV) RNA, isolated as virion RNA from purified virus particles or as total RNA from infected cells, has been microinjected into nuclei and cytoplasms of BHK cells. When injected directly into the nucleus FMDV RNA was not infectious, whereas cytoplasmic injection resulted in a high proportion of productive infections. Infectivity microinjection assays on dilution series of various FMDV RNAs showed that both single-stranded positive sense 35S RNA and double-stranded replicative form (Rf) RNA have an infectivity close to 1 p.f.u. per molecule, although only a minor fraction of BHK cells appeared able to support plaque formation following microinjection of Rf FMDV RNA. The infectivity of Rf FMDV RNA was not sensitive to inhibition by actinomycin D. The results are discussed in relation to the high virus particle to p.f.u. ratios observed for FMDV.
BACHRACHH. L., TRAUTMANR., BREESES. S.1964; Chemical and physical properties of virtually pure foot-and-mouth disease virus. American Journal of Veterinary Research 25:333–342
BISHOPJ. M., KOCHG.1967; Purification and characterization of poliovirus-induced infectious double-stranded ribonucleic acid. Journal of Biological Chemistry 242:1736–1743
DETJENB. M., LUCASJ., WIMMERE.1978; Poliovirus single-stranded RNA and double-stranded RNA: differential infectivity in enucleate cells. Journal of Virology 27:582–586
GRAESSMANNM., GRAESSMANA.1986 Microinjection of tissue culture cells using glass capillaries: methods. Microinjection and Organelle Transplantation Techniques, Methods and Applications3–13 Edited by CelisJ. E., GraessmannA., LoyterA. London: Academic Press;
HEWLETTM. J., ROZENBLATTS., AMBROSV., BALTIMORED.1977; Separation and quantitation of intracellular forms of poliovirus RNA by agarose gel electrophoresis. Biochemistry 16:2763–2767
KOCHG., QUINTRELLN., BISHOPJ. M.1967; Differential effect of actinomycin D on the infectivity of single and double-stranded poliovirus RNA. Virology 31:388–390
KUHNR. J., WIMMERE.1987 The replication of picornaviruses. The Molecular Biology of the Positive Strand Viruses, FEMS Symposium3217–51 Edited by RowlandsD. J., MayoM. A., MahyB. W. J. London: Academic Press;
RUECKERTR. R.1976 On the structure and morphogenesis of picornaviruses. Comprehensive Virology6131–213 Edited by Fraenkel-ConratH., WagnerR. R. London: Plenum Press;
VAN DER WERFS., BRADLEYJ., WIMMERE., STUDIERF. W., DüNNJ. L.1986; Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proceedings of the National Academy”of Sciences, U.S.A 83:2330–2334
XIEQ-C, McCAHOND., CROWTHERJ. R., BELSHAMG. J., McCULLOUGHK. C.1987; Neutralization of foot-and-mouth disease virus can be mediated through any of at least three separate antigenic sites. Journal of General Virology 68:1637–1647