Reports on the arrest of herpes simplex virus type 1 (HSV-1) replication by interferon (IFN) are inconsistent. By the use of immunofluorescence and immunoblot assays with monoclonal and polyclonal antibodies, effective arrest of viral translation by human IFN-α in human fibroblasts was detected for the HSV-1 strains KOS and McIntyre. In HeLa cells which are less sensitive to IFN inhibition and in 444 cells, a HeLa-fibroblast hybrid cell line, the inhibition was less pronounced. These results confirm earlier observations that IFN or polyinosinic-polycytidylic acid block the replication of HSV-1 in human, monkey and mouse cells no later than the immediate early phase of infection.
BRANDNERG., MUELLERN.1973; Wirkung von Interferon und poly(I): poly(C) auf Genomfunktionen von Herpes-simplex-Virus Typ 1. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 354:1176
CHATTERJEES., HUNTERE., WHITLEYR.1985; Effect of cloned human interferons on protein synthesis and morphogenesis of herpes simplex virus. Journal of Virology 56:419–425
DOMKE-OPITZI., STRAUBP., KIRCHNERH.1986; Effect of interferon on replication of herpes simplex virus types 1 and 2 in human macrophages. Journal of Virology 60:37–42
LEWISJ. A., MENGHERIG., ESTEBANM.1983; Induction of an antiviral response by interferon requires thymidine kinase. Proceedings of the National Academy of Sciences, U.S.A 80:26–30
LIPPM.1981Wirkung von Interferon auf die Genexpression von Herpes-simplex-Virus und Epstein-Barr-Virus in lytisch infizierten und transformierten Zellen Ph.D. thesis, University of Freiburg
LIPPM., BRANDNERG.1980; Inhibition of herpes simplex virus type 1 specific translation in cells treated with poly(rI).poly(rC). Journal of General Virology 47:97–111
LIPPM., BRANDNERG.1985 Herpes simplex virus gene expression in interferon-treated cells. The Biology of the Interferon System355–360 Edited by KirchnerH., SchellekensH. Amsterdam: Elsevier;
MÜNOZA., CARRASCOL.1984; Formation of non-infective herpesvirus particles in cultured cells treated with human interferon. Journal of General Virology 65:1069–1078
PANETA., FALKH.1983; Inhibition by interferon of herpes simplex virus thymidine kinase and DNA polymerase in infected and biochemically transformed cells. Journal of General Virology 64:1999–2006
PANETA., GLOGERI., FALKH.1985 Mechanisms of herpes simplex virus inhibition by interferon. The Biology of the Interferon System325–331 Edited by KirchnerH., SchellekensH. Amsterdam: Elsevier;
SAKURAGIA. L. Z., SIMONE. H.1986; Selection and characterization of an interferon-responsive clonal cell line of HeLa cells. Journal of General Virology 67:2355–2364
SHOWALTERS. D., ZWEIGM., HAMPARB.1981; Monoclonal antibodies to herpes simplex type 1 proteins, including the immediate early protein ICP 4. Infection and Immunity 34:684–692