1887

Abstract

Summary

hybridization with synthetic oligonucleotides was used to fine map the extent and continuity of herpes simplex virus type 1 (HSV-1) latency-related RNA (LR RNA) in trigeminal ganglia of latently infected humans. A series of 34 20 residue oligonucleotides were employed to cover a 3 kb region of the HSV-1 genome to which the latency-related gene had previously been mapped. The 5′ end of the main LR RNA transcript began approximately 1210 nucleotides downstream from the 3′ end of the mRNA from the immediate early gene ICP0. The 3′ end of the LR RNA overlapped the 3′ end of the ICP0 mRNA by approximately 1000 nucleotides. A potential small intron was detected near the 3′ end of the LR RNA.

Keyword(s): HSV-1 , latency and mapping
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-12-3101
1988-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/12/JV0690123101.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-12-3101&mimeType=html&fmt=ahah

References

  1. CROEN K. D., OSTROVE J. M., DRAGOVIC L. I., SMIALEK J. E., STRAUS S. E. 1987; Latent herpes simplex virus in human trigeminal ganglia: detection of an immediate early gene ‘antisense’ transcript by in situ hybridization. New England Journal of Medicine 317:1427–1432
    [Google Scholar]
  2. DEATLY A. M., SPIVACK J. G., LAVI E., FRASER N. W. 1987; RNA from an immediate early region of the type 1 herpes simplex virus genome is present in the trigeminal ganglia of latently infected mice. Proceedings of the National Academy of Sciences, U.S.A 84:3204–3208
    [Google Scholar]
  3. PERRY L. J., RIXON F. J., EVERETT R. D., FRAME M. C., MCGEOCH D. J. 1987; Characterization of the IE110 gene of herpes simplex virus type 1. Journal of General Virology 67:2365–2380
    [Google Scholar]
  4. PUGA A., NOTKINS A. L. 1987; Continued expression of a poly(A)+ transcript of herpes simplex virus type 1 in trigeminal ganglia of latently infected mice. Journal of Virology 61:1700–1703
    [Google Scholar]
  5. ROCK D. L., HAGEMOSER W. A., OSORIO F. A., REED D. E. 1986; Detection of bovine herpesvirus type 1 RNA in trigeminal ganglia of latently infected rabbits by in situ hybridization. Journal of General Virology 67:2515–2520
    [Google Scholar]
  6. ROCK D. L., NESBURN A. B., GHIASI H., ONG J., LEWIS T. L., LOKENSGARD J. R., WECHSLER S. L. 1987; Detection of latency related viral RNAs in trigeminal ganglia of rabbits latently infected with herpes simplex virus type 1. Journal of Virology 61:3820–3826
    [Google Scholar]
  7. SPIVACK J. G., FRASER N. W. 1987; Detection of herpes simplex virus type 1 transcripts during latent infection in mice. Journal of Virology 61:3841–3847
    [Google Scholar]
  8. STEVENS J. G., WAGNER E. K., DEVI-RAO G. B., COOK M. L., FELDMAN L. T. 1987; RNA complementary to a herpesvirus alpha gene mRNA is prominent in latently infected neurons. Science 235:1056–1059
    [Google Scholar]
  9. STROOP W. G., ROCK D. L., FRASER N. W. 1984; Localization of herpes simplex virus in the trigeminal and olfactory systems of the mouse central nervous system during acute and latent infections by in situ hybridization. Laboratory Investigation 51:27–38
    [Google Scholar]
  10. WAGNER E. K., DEVI-RAO G., FELDMAN L. T., DOBSON A. T., ZHANG Y., FLANAGAN W. M., STEVENS J. G. 1988; Physical characterization of the herpes simplex virus latency-associated transcript in neurons. Journal of Virology 62:1194–1202
    [Google Scholar]
  11. WECHSLER S. L., NESBURN A. B., WATSON R. J., SLANINA S. M., GHIASI H. 1988; Fine mapping of the latency related gene of herpes simplex virus type 1: alternative splicing produces distinct latency related-RNAs containing open reading frames. Journal of Virology 62: in press
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-12-3101
Loading
/content/journal/jgv/10.1099/0022-1317-69-12-3101
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error