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1887

Journal of General Virology header logo Journal of General Virology

Volume 69, Issue 12

Citrus Cachexia Viroid, a New Viroid of Citrus: Relationship to Viroids of the Exocortis Disease Complex No Access

Abstract

Summary

Recovery of highly purified citrus cachexia viroid (CCaV) was accomplished by serial elution following CF-11 cellulose chromatography of a 2 -LiCl-soluble nucleic acid preparation. The alternative herbaceous host, cucumber ( cv. Suyo), yielded greater quantities of the viroid than the highest yielding citrus host, citron ( cv. Etrog). A randomly primed cDNA probe to CCaV purified from cucumber reacted positively to extracts from citron and cucumber inoculated with the same isolate of CCaV. When tested against a broad range of other citrus viroids, the CCaV cDNA hybridized to only one, CV-IIa, which has been identified as the causal agent of a mild form of the citrus exocortis disease. Because of the apparent homology between the nucleotide sequences of CV-IIa and CCaV, and a size difference of only five to ten nucleotides, these RNAs can be considered as members of a common subgroup of citrus viroids. These two viroids have been classified by bioassay reactions as the causal agents of two distinct types of citrus disease, an ‘exocortis-like’ syndrome and cachexia. The properties of and relationships between these two members of the citrus viroid II group and the definition of the exocortis and cachexia (xyloporosis) diseases are presented.

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Keyword(s): citrus cachexia viroid , exocortis disease and viroids
© Society for General Microbiology 1988
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1988-12-01
2025-06-19
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References

  1. CHILDS J. F. L. 1950; The cachexia disease of Orlando tangelo. Plant Disease Reporter 34:295–298
     [Google Scholar]
  2. CHILDS J. F. L. 1952; Cachexia disease: its bud transmission and relation to xyloporosis and tristeza. Phytopathology 42:265–268
     [Google Scholar]
  3. DURAN-VILA N., FLORES R., SEMANCIK J. S. 1986; Characterization of viroid-like RNAs associated with the citrus exocortis syndrome. Virology 150:75–84
     [Google Scholar]
  4. DURAN-VILA N., PINA J. A., BALLESTER J. F., JUAREZ J., ROISTACHER C. N., RIVERA-BUSTAMANTE R., SEMANCIK J. S. 1988; The citrus exocortis disease: a complex of viroid RNAs. Proceedings of the International Organization of Citrus Virologists 10:152–164
     [Google Scholar]
  5. GARGER S. J., TURPEN T., CARRINGTON J. C, MORRIS T. J., DODDS I. A., GRILL L. K. 1983; Rapid detection of plant viruses by dot blot hybridization. Plant Molecular Biology Reporter 1:21–25
     [Google Scholar]
  6. HALL T. C, WEPPRICH R. K., DAVIES J. W., WEATHERS L. G., SEMANCIK J. S. 1974; Functional distinctions between the ribonucleic acids from citrus exocortis viroid and plant viruses: cell-free translation and aminoacylation reactions. Virology 61:486–492
     [Google Scholar]
  7. IGLOI G. L. 1983; A silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis. Analytical Biochemistry 134:184–188
     [Google Scholar]
  8. LIN J. J., SEMANCIK J. S. 1985; Coordination between host nucleic acid metabolism and citrus exocortis viroid turnover. Virus Research 3:213–230
     [Google Scholar]
  9. MANIATIS T., FRITSCH E. F., SAMBROOK T. 1982 Molecular Cloning: A Laboratory Manual129–132230–234 New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  10. MARTON L., DURAN-VILA N., LIN J.-J., SEMANCIK J. S. 1982; Properties of cell cultures containing the citrus exocortis viroid. Virology 122:229–238
     [Google Scholar]
  11. RIVERA-BUSTAMANTE R., GIN R., SEMANCIK J. S. 1986; Enhanced resolution of circular and linear molecular forms of viroid and viroid-like RNA by electrophoresis in a discontinuous-pH system. Analytical Biochemistry 156:91–95
     [Google Scholar]
  12. ROISTACHER C N. 1988; Cachexia and xyloporosis diseases. A review. Proceedings of the International Organization of Citrus Virologists 10:116–124
     [Google Scholar]
  13. ROISTACHER C. N., BLUE R. L., CALAVAN E. C. 1973; A new test for citrus cachexia. Citrograph 58:261–262
     [Google Scholar]
  14. ROISTACHER C. N., CALAVAN E. C, BLUE R. L., NAVARRO L., GONZALES R. 1977; A new more sensitive citron indicator for detection of mild isolates of citrus exocortis viroid (CEV). Plant Disease Reporter 53:333–336
     [Google Scholar]
  15. ROISTACHER C. N., GUMPF D. J., NAUER E. M., GONZALES R. 1983; Cachexia disease: virus or viroid. Citrograph 68:111–113
     [Google Scholar]
  16. SÄNGER H. L. 1972; An infectious and replicating RNA of low molecular weight: the agent of the exocortis disease of citrus. Advances in Bioscience 8:103–116
     [Google Scholar]
  17. SANO T., HATAYA T., SASAKI A., SHIKATA T. 1986; Etrog citron is latently infected with hop stunt viroid-like RNA. Proceedings of the Japan Academy 62:325–328
     [Google Scholar]
  18. SANO T., HATAYA T., SHIKATA T. 1988; Complete nucleotide sequence of a viroid isolated from Etrog citron, a new member of hop stunt viroid group. Nucleic Acids Research 16:347
     [Google Scholar]
  19. SCHLEMMER A., ROISTACHER C. N., SEMANCIK J. S. 1985; A unique, infectious RNA which causes symptoms in citron typical of citrus exocortis disease. Phytopathology 75:946–949
     [Google Scholar]
  20. SEMANCIK J. S. 1979; Small pathogenic RNA in plants - the viroids. Annual Review of Phytopathology 17:461–484
     [Google Scholar]
  21. SEMANCIK J. S. 1986; Separation of viroid RNAs by cellulose chromatography indicating conformational distinctions. Virology 155:39–45
     [Google Scholar]
  22. SEMANCIK J. S. 1988; The citrus exocortis disease. A review 1976-1986. Proceedings of the International Organization of Citrus Virologists 10:136–151
     [Google Scholar]
  23. SEMANCIK J. S., HARPER K. L. 1984; Optimal conditions for cell-free synthesis of citrus exocortis viroid and the question of specificity of RNA polymerase activity. Proceedings of the National Academy of Sciences, U.S.A 81:4429–4433
     [Google Scholar]
  24. SEMANCIK J. S., WEATHERS L. G. 1972; Exocortis disease: evidence for a new species of ‘infectious’ low molecular weight RNA in plants. Nature New Biology 237:242–244
     [Google Scholar]
  25. SEMANCIK J. S., MORRIS T. J., WEATHERS L. G., RORDORF G. F., KEARNS D. R. 1975; Physical properties of a minimal infectious RNA (viroid) associated with the exocortis disease. Virology 63:160–167
     [Google Scholar]
  26. SEMANCIK J. S., CONEJERO V., GERHART J. 1977; Citrus exocortis viroid: survey of protein synthesis in Xenopus laevis oocytes following addition of viroid RNA. Virology 80:218–221
     [Google Scholar]
  27. SEMANCIK J. S., ROISTACHER C. N., DURAN-VILA N. 1988; A new viroid is the causal agent of the citrus cachexia disease. Proceedings of the International Organization of Citrus Virologists 10:125–135
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-69-12-3059
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Citrus Cachexia Viroid, a New Viroid of Citrus: Relationship to Viroids of the Exocortis Disease Complex
J. Gen. Virol. 69, 3059 (1988); https://doi.org/10.1099/0022-1317-69-12-3059
/content/journal/jgv/10.1099/0022-1317-69-12-3059
/content/journal/jgv/10.1099/0022-1317-69-12-3059
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