1887

Abstract

Summary

Macrophages (Mφ) harvested from the peritoneal cavities of mice after thioglycollate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected Mφ were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected Mφ produced moderate amounts of IFN, which was completely neutralized by anti-IFN-α/β serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing Mφ to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the Mφ cultures with anti-IFN-α/β resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of Mφ releasing MCMV (VR-Mφ). Thus, the IFN produced in MCMV-infected Mφ (MCMV-Mφ IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-Mφ observed was more resistant to anti-IFN-α/β treatment than the production of infectious virus. The antiviral effect of MCMV-Mφ IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-α/β. Therefore, MCMV-Mφ IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that Mφ may have a role in the latency of MCMV and that their production of IFN may facilitate the generation of latent infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-12-2961
1988-12-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/12/JV0690122961.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-12-2961&mimeType=html&fmt=ahah

References

  1. AZUMA M. 1976; Viral factors required for interferon induction by Newcastle disease virus in mouse macrophages and chicken embryo cells. Journal of General Virology 30:51–62
    [Google Scholar]
  2. BIXLER G. S., BOOSS J. 1981; Adherent spleen cells from mice acutely infected with cytomegalovirus suppress the primary antibody response. in vitro. Journal of Immunology 127:1294–1299
    [Google Scholar]
  3. BOOSS J. 1980; Establishment of cytomegaloviral infection in mice: role of a macrophage-enriched subpopulation. Journal of Infectious Diseases 141:466–472
    [Google Scholar]
  4. BRÄUTIGAM A. R., DUTKO F. J., OLDING L. B., OLDSTONE M. B. A. 1979; Pathogenesis of murine cytomegalovirus infection: the macrophage as a permissive cell for cytomegalovirus infection, replication and latency. Journal of General Virology 44:349–359
    [Google Scholar]
  5. CHONG K. T., GRESSER I., MIMS C. A. 1983; Interferon as a defence mechanism in mouse cytomegalovirus infection. Journal of General Virology 64:461–464
    [Google Scholar]
  6. DREW W. L., MINTZ L., HOO R., FINLEY T. N. 1979; Growth of herpes simplex and cytomegalovirus in cultured human alveolar macrophages. American Review of Respiratory Disease 119:287–291
    [Google Scholar]
  7. ETTENSOHN D. B., ROBERTS N. J. JR 1984; Influenza virus infection of human alveolar and blood-derived macrophages: differences in accessory cell function and interferon production. Journal of Infectious Diseases 149:942–949
    [Google Scholar]
  8. FERTSCH D., VOGEL J. N. 1984; Recombinant interferons increase macrophage receptor capacity. Journal of Immunology 132:2436–2439
    [Google Scholar]
  9. GLASGOW L. A., HABEL K. 1963; Interferon production by mouse leukocytes in vitro and. in vivo. Journal of Experimental Medicine 117:149–161
    [Google Scholar]
  10. GRUNDY (CHALMER) J. E., TRAPMAN J., ALLAN J. E., SHELLAM G. R., MELIEF C. J. M. 1982; Evidence for a protective role of interferon in resistance to murine cytomegalovirus and its control by non-H-2-linked genes. Infection and Immunity 37:143–150
    [Google Scholar]
  11. HENSON D., SMITH R. D. 1964; Interferon production in vitro by cells infected with the murine salivary gland virus. Proceedings of the Society for Experimental Biology and Medicine 117:517–520
    [Google Scholar]
  12. INADA T., MIMS C. A. 1985; Association of virulence of murine cytomegalovirus with macrophage susceptibility and with virion-bound non-neutralizing antibody. Journal of General Virology 66:879–882
    [Google Scholar]
  13. INADA T., CHONG K. T., MIMS C. A. 1985; Enhancing antibodies, macrophages and virulence in mouse cytomegalovirus infection. Journal of General Virology 66:871–878
    [Google Scholar]
  14. ISAACS A., BARON S. 1960; Antiviral action of interferon in embryonic cells. Lancet ii:946–947
    [Google Scholar]
  15. JORDAN M. C., MAR V. L. 1982; Spontaneous activation of latent cytomegalovirus from murine spleen expiants. Journal of Clinical Investigation 70:762–768
    [Google Scholar]
  16. KATZENSTEIN D. A., YU G. S. M., JORDAN M. C. 1983; Lethal infection with murine cytomegalovirus after early viral replication in the spleen. Journal of Infectious Diseases 148:406–411
    [Google Scholar]
  17. LAGWINSKA E., STEWART C. C, ADLES C., SCHLESINGER S. 1975; Replication of lactic dehydrogenase virus and Sindbis virus in mouse peritoneal macrophages. Induction of interferon and phenotypic mixing. Virology 65:204–214
    [Google Scholar]
  18. LANG D. J., THOMAS M., GRESSER I. 1969; Protection par l’interferon de cellules embryonnaires humaines contres l’infection par le virus cytomegalique. Comptes rendus hebdomadaires des séances de l’Académie des sciences D268:3137–3139
    [Google Scholar]
  19. LODMELL D. L., NIWA A., HAYASHI K., NOTKINS A. L. 1978; Prevention of cell-to-cell spread of herpes simplex virus by leukocytes. Journal of Experimental Medicine 137:706–720
    [Google Scholar]
  20. MCGAVRAN M. H., SMITH M. G. 1965; Ultrastructural, cytochemical, and microchemical observations on cytomegalovirus (salivary gland virus) infection of human cells in tissue culture. Experimental and Molecular Pathology 4:1–10
    [Google Scholar]
  21. MIMS C. A., GOULD J. 1978; The role of macrophages in mice infected with murine cytomegalovirus. Journal of General Virology 41:143–153
    [Google Scholar]
  22. MORAHAN P. S., MORSE S. S., MCGEORGE M. B. 1980; Macrophage extrinsic antiviral activity during herpes simplex virus infection. Journal of General Virology 46:291–300
    [Google Scholar]
  23. MORSE S. S., MORAHAN P. S. 1981; Activated macrophages mediate interferon-independent inhibition of herpes simplex virus. Cellular Immunology 58:72–84
    [Google Scholar]
  24. NUGENT K. M., PESANTI E. L. 1979; Effect of influenza infection on the phagocytic and bactericidal activities of pulmonary macrophages. Infection and Immunity 26:651–657
    [Google Scholar]
  25. OIE H. K., EASTON J. M., ABLASHI D. V., BARON S. 1975; Murine cytomegalovirus: induction of and sensitivity to interferon. in vitro. Infection and Immunity 12:1012–1017
    [Google Scholar]
  26. SAWICKI L. 1961; Influence of age of mice on the recovery from experimental Sendai virus infection. Nature, London 192:1258–1259
    [Google Scholar]
  27. SCHULTZ R. M. 1980; Macrophage activation by interferons. Lymphokine Report 1:63–97
    [Google Scholar]
  28. SCHULTZ R. M., CHIRIGOS M. A., HEINE U. I. 1978; Functional and morphologic characteristics of interferon-treated macrophages. Cellular Immunology 35:84–91
    [Google Scholar]
  29. SELGRADE M. K., OSBORN J. E. 1974; Role of macrophages in resistance of murine cytomegalovirus. Infection and Immunity 10:1383–1390
    [Google Scholar]
  30. SERGIESCU D. 1983; Interferon induced by UV-irradiated murine cytomegalovirus decreases type C virus expression in BALB/c cells treated by 5-iodo-deoxyuridine or cycloheximide. Journal of Interferon Research 3:451–460
    [Google Scholar]
  31. SHANLEY J. D., PESANTI E. L. 1982; Effects of antiviral agents on murine cytomegalovirus-induced macrophage dysfunction. Infection and Immunity 36:918–923
    [Google Scholar]
  32. SHANLEY J. D., PESANTI E. L. 1983; Murine peritoneal macrophages support murine cytomegalovirus replication. Infection and Immunity 41:1352–1359
    [Google Scholar]
  33. SHANLEY J. D., JORDAN M. C, COOK M. L., STEVENS J. G. 1979; Pathogenesis of reactivated latent murine cytomegalovirus infection. American Journal of Pathology 95:67–79
    [Google Scholar]
  34. SHANLEY J. D., JORDAN M. C., STEVENS J. C. 1981; Modification by adoptive humoral immunity of murine cytomegalovirus infection. Journal of Infectious Diseases 143:231–237
    [Google Scholar]
  35. STEBBING N., DAWSON K. M., LINDLEY I. J. D. 1978; Requirement for macrophages for interferon to be effective against encephalomyocarditis virus infection of mice. Infection and Immunity 19:5–11
    [Google Scholar]
  36. STEVENS J. G., COOK M. L. 1971; Restriction of herpes simplex virus by macrophages. An analysis of the cell-virus interaction. Journal of Experimental Medicine 133:19–38
    [Google Scholar]
  37. TÁLAS M., KONSTANTINOVA I, FUKS B. B., STÖGER I., SZOLGAY E. 1973; Interferon, nucleic acid and protein synthesis in mouse spleen cells after acute γ-irradiation. Journal of General Virology 18:393–397
    [Google Scholar]
  38. TEGTMEYER P. J., CRAIGHEAD J. E. 1968; Infection of adult mouse macrophages in vitro with cytomegalovirus. Proceedings of the Society for Experimental Biology and Medicine 129:690–694
    [Google Scholar]
  39. TSUKUI K. 1977; Influenza virus-induced interferon production in mouse spleen cell culture: T cells as the main producer. Cellular Immunology 32:243–251
    [Google Scholar]
  40. VACZI L., HORVATH E., HADHZY G. 1966; Studies on the conditions of interferon production by cells infected with herpesviruses. Acta microbiologica Academiae scientiarum Hungaricae 122:345–349
    [Google Scholar]
  41. VOGEL S. N., FINBLOOM D. S., ENGLISH K. E., ROSENSTREICH D. L., LANGRETH S. G. 1983; Interferon-induced enhancement of macrophage Fc receptor expression: β-interferon treatment of C3H/HeS macrophages results in increased numbers and chemistry of Fc receptors. Journal of Immunology 130:1210–1214
    [Google Scholar]
  42. WILDY P., GELL P. G. H., RHODES J., NEWTON A. 1982; Inhibition of herpes simplex virus multiplication by activated macrophages: a role for arginase?. Infection and Immunity 37:40–45
    [Google Scholar]
  43. YAMAGUCHI T., KURODA Y., SAITO M., EBINA T., HOSHINO F., ISHIDA N. 1984; Immune interferon production by TH69, a lyophilized preparation of Streptococcus faecalis, in murine spleen cell cultures. Microbiology and Immunology 28:601–610
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-12-2961
Loading
/content/journal/jgv/10.1099/0022-1317-69-12-2961
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error