1887

Journal of General Virology header logo

Volume 69, Issue 11

Characterization of Papillomavirus Polypeptides from Bovine Cutaneous Fibropapillomas Free

Abstract

Summary

Virions of bovine papilloma virus (BPV) were isolated from a pool of cutaneous bovine fibropapillomas and purified by CsCl gradient centrifugation. SDS-PAGE revealed several polypeptides with an ranging from 76K to 19K. Western blot analysis of the viral isolate identified additional polypeptides when a rabbit anti-BPV serum was used, but only the main capsid component of 57K when a rabbit antiserum raised against human papillomavirus was used. The viral preparation was then I-labelled and further purified by gel filtration. SDS-PAGE of immunoprecipitates of the anti-BPV serum with different fractions from the chromatographic column revealed the polypeptides of 76K, 57K and 28K to be viral structural components. The 28K polypeptide, not previously characterized, was shown to be composed of several molecular forms, migrating over a pH range of 3.5 to 4.6 when analysed by two-dimensional PAGE. Following SDS-PAGE performed under non-reducing conditions, the 28K and 76K polypeptides and the main capsid component of 57K appeared to be linked by disulphide bridges to form hetero- or homopolymers.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BPV , capsid proteins and fibropapillomas
© Society for General Microbiology 1988
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-11-2919
1988-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/11/JV0690112919.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-11-2919&mimeType=html&fmt=ahah

References

  1. CLEVELAND D. W., FISCHER S. G., KIRSCHNER M. W., LAEMMLI U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
     [Google Scholar]
  2. DANOS O., GIRI I., THIERRY F., YANLV M. 1984; Papillomavirus genomes: sequences and consequences. Journal of Investigative Dermatology 83:7s–11s
     [Google Scholar]
  3. FAVRE M., BREITBURD F., CROISSANT O., ORTH G. 1975; Structural polypeptides of rabbit, bovine, and human papillomaviruses. Journal of Virology 15:1239–1247
     [Google Scholar]
  4. FAVRE M., BREITBURD F., CROISSANT O., ORTH G. 1977; Chromatin-like structures obtained after alkaline disruption of bovine and human papillomaviruses. Journal of Virology 21:1205–1209
     [Google Scholar]
  5. GISSMANN L., PFISTER H., ZUR HAUSEN H. 1977; Human papilloma viruses (HPV): characterization of four different isolates. Virology 76:569–580
     [Google Scholar]
  6. GORRA J. B., LANCASTER W. D., KURMAN R. J., JENSON A. B. 1985; Bovine papillomavirus type 1 monoclonal antibodies. Journal of the National Cancer Institute 75:121–125
     [Google Scholar]
  7. HUNTER W. M., GREENWOOD F. C. 1962; Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194:495–496
     [Google Scholar]
  8. JENSON A. B., ROSENTHAL J. D., OLSON C, PASS F., LANCASTER W. D., SHAH K. 1980; Immunologic relatedness of papillomaviruses from different species. Journal of the National Cancer Institute 64:495–500
     [Google Scholar]
  9. KOMLY C. A., BREITBURD F., CROISSANT O., STREECK R. E. 1986; The L2 open reading frame of human papillomavirus type 1a encodes a minor structural protein carrying type-specific antigens. Journal of Virology 60:813–816
     [Google Scholar]
  10. LANCASTER W. D., OLSON C., MEINKE W. 1967; Quantitation of bovine papilloma virus DNA in viral-induced tumors. Journal of Virology 17:824–831
     [Google Scholar]
  11. MEINKE W., MEINKE G. C. 1981; Isolation and characterization of bovine papillomavirus type 1. Journal of General Virology 52:15–24
     [Google Scholar]
  12. NAKAI Y., LANCASTER W. D., LIM L. Y., JENSON A. B. 1986; Monoclonal antibodies to genus and type-specific papillomavirus structural antigens. Intervirology 25:30–37
     [Google Scholar]
  13. O’FARRELL P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
     [Google Scholar]
  14. O’FARRELL P. Z., GOODMAN H. M. 1976; Resolution of simian virus 40 proteins in whole cell extracts by two-dimensional electrophoresis: heterogeneity of the major capsid protein. Cell 9:289–298
     [Google Scholar]
  15. ORTH G., FAVRE M., CROISSANT O. 1977; Characterization of a new type of human papillomavirus that causes skin warts. Journal of Virology 24:108–120
     [Google Scholar]
  16. ORTH G., BREITBURD F., FAVRE M. 1978; Evidence for antigenic determinants shared by the structural polypeptides of (Shope) rabbit papillomavirus and human papillomavirus type 1. Virology 91:243–255
     [Google Scholar]
  17. PFISTER H., GISSMANN L., ZUR HAUSEN H. 1977; Partial characterization of the proteins of human papilloma viruses (HPV) 1-3. Virology 83:131–137
     [Google Scholar]
  18. PFISTER H., LINZ U., GISSMANN L., HUCHTHAUSEN B., HOFFMANN D., ZUR HAUSEN H. 1979; Partial characterization of a new type of bovine papillomavirus. Virology 96:1–8
     [Google Scholar]
  19. PILACINSKI W. P., GLASSMAN D. L., KRZYZEK R. A., SADOWSKI P. L., RABBINS A. K. 1984; Cloning and expression in Escherichia coli of the bovine papillomavirus L1 and L2 open reading frames. Bio Technology 2:356–360
     [Google Scholar]
  20. PONDER B. A. J., CRAWFORD L. V. 1977; The arrangement of nucleosomes in nucleoprotein complexes from polyoma virus and SV40. Cell 11:45–49
     [Google Scholar]
  21. ROSETO A., POTHIER P., GUILLEMIN M.-C, PERIES J., BREITBURD F., BONNEAUD N., ORTH G. 1984; Monoclonal antibodies to the major capsid protein of human papillomavirus type 1. Journal of General Virology 65:1319–1324
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-11-2919
Loading
Characterization of Papillomavirus Polypeptides from Bovine Cutaneous Fibropapillomas
J. Gen. Virol. 69, 2919 (1988); https://doi.org/10.1099/0022-1317-69-11-2919
/content/journal/jgv/10.1099/0022-1317-69-11-2919
/content/journal/jgv/10.1099/0022-1317-69-11-2919
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error