1887

Abstract

Summary

Analysis of the 136 possible pairings of the coat protein amino acid sequences from 17 strains of eight distinct potyviruses revealed a bimodal distribution of sequence homology. Distinct members of the group exhibited sequence homologies ranging from 38 to 71% (average 54%) with major differences in the length and sequence of their N termini and high sequence homology in the C-terminal half of the coat proteins. In contrast strains of individual viruses exhibited sequence homologies of 90 to 99% (average 95%) and had very similar N-terminal sequences. These findings cast doubt on the currently held ‘continuum’ hypothesis proposed to explain the unsatisfactory taxonomy of the potyvirus group. The coat protein sequence data, in combination with information on the nature of the potyvirus particle assembly, can be used to develop rationally designed, simple serological techniques that appear to be more useful and more easily applied than those properties previously used for potyvirus identification and classification.

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1988-11-01
2022-01-28
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References

  1. ALLISON R. F, DOUGHERTY W. G., PARKS T. D., WILLIS L., JOHNSTON R. E., KELLY M. E., ARMSTRONG F. B. 1985a; Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion’s surface. Virology 147:309–316
    [Google Scholar]
  2. ALLISON R. F., SORENSON J. C, KELLY M. E., ARMSTRONG F. B., DOUGHERTY W. G. 1985b; Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: evidence for the synthesis and processing of a polyprotein in potyvirus genome expression. Proceedings of the National Academy of Sciences, U.S.A 82:3969–3972
    [Google Scholar]
  3. BOS L. 1970; The identification of three new viruses isolated from Wisteria and Pisum in The Netherlands, and the problems of variation within the potato virus Y group. Netherlands Journal of Plant Pathology 76:8–46
    [Google Scholar]
  4. BROWN F. 1986; The classification and nomenclature of viruses. Summary of results of meetings of the International Committee on Taxonomy of Viruses in Sendai, September, 1984. Intervirology 25:141–143
    [Google Scholar]
  5. COLMAN P. M., WARD C. W. 1985; Structure and diversity of influenza virus neuraminidase. Current Topics in Microbiology and Immunology 114:177–255
    [Google Scholar]
  6. DALE B., BROWN R., MILLER J., WHITE R. T., AIR G. M., CORDELL B. 1986; Nucleotide and deduced amino acid sequence of the influenza neuraminidase genes of two equine serotypes. Virology 155:460–468
    [Google Scholar]
  7. DOMIER L. L., FRANKLIN K. M., SHAHBUDDIN M., HELLMAN G. M., OVERMEYER J. H., HIREMATH S. T., SIAW M. E. E., LOMONOSSOFF G. P., SHAW I. G., RHOADS R. E. 1986; The nucleotide sequence of tobacco vein mottling virus. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  8. DOMIER L. L., SHAW J. G., RHOADS R. E. 1987; Potyviral proteins share amino acid sequence homology with picorna-, como-, and caulimoviral proteins. Virology 158:20–27
    [Google Scholar]
  9. DOUGHERTY W. G., ALLISON R. F., PARKS T. D., JOHNSTON R. E., FEILD M. J., ARMSTRONG F. B. 1985; Nucleotide sequence at the 3′-terminus of pepper mottle virus genomic RNA: evidence for an alternative mode of capsid protein gene organization. Virology 146:282–291
    [Google Scholar]
  10. EDWARDSON J. R. 1974; Some properties of the potato virus Y-group. Monograph Series, Florida Agricultural Experimental Station 4:
    [Google Scholar]
  11. EGGENBERGER A. L., STARK D. M., BEACHY R. N. 1988; Nucleotide sequence and expression of the soybean mosaic virus coat protein coding region in Escherichia coli and tobacco callus. Virology in press
    [Google Scholar]
  12. FAUQUET C, DEJARDIN J., THOUVENEL J.-C. 1986a; Evidence that the amino acid composition of the particle proteins of plant viruses is characteristic of the virus group. 1. Multidimensional classification of plant viruses. Intervirology 25:1–13
    [Google Scholar]
  13. FAUQUET C, DEJARDIN J., THOUVENEL J.-C. 1986b; Evidence that the amino acid composition of the particle proteins of plant viruses is characteristic of the virus group. 2. Discriminant analysis according to structural biological and classification properties of plant viruses. Intervirology 25:190–200
    [Google Scholar]
  14. FRANCKI R. I. B. 1983 Current problems in plant virus taxonomy. A Critical Appraisal of Viral Taxonomy63–104 Edited by Matthews R. E. F. Boca Raton: CRC Press;
    [Google Scholar]
  15. FRANCKI R. I. B., MILNE R. G., HATTA T. 1985 Atlas of Plant Viruses I and II Boca Raton: CRC Press;
    [Google Scholar]
  16. GIBBS A. J. 1977; Tobamovirus group. CMI/AAB Descriptions of Plant Viruses184
    [Google Scholar]
  17. GOUGH K. H., SHUKLA D. D. 1981; Coat protein of potyviruses. 1. Comparison of the four Australian strains of sugarcane mosaic virus. Virology 111:455–462
    [Google Scholar]
  18. GUNYUZLU P. L., TOLIN S. A., JOHNSON J. L. 1987; The nucleotide sequence of the 3′-terminus of soybean mosaic virus. Phytopathology 77:1766
    [Google Scholar]
  19. HARRISON B. D. 1985; Usefulness and limitations of the species concept for plant viruses. Intervirology 25:71–78
    [Google Scholar]
  20. HIEBERT E., PURCIFULL D. E., CHRISTIE R. G. 1984; Purification and immunological analysis of plant viral inclusion bodies. Methods in Virology 8:225–280
    [Google Scholar]
  21. HOLLINGS M., BRUNT A. A. 1981a Potyviruses. Handbook of Plant Virus Infections: Comparative Diagnosis731–807 Edited by Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  22. HOLLINGS M., BRUNT A. A. 1981b; Potyvirus group. CMI/AAB Descriptions of Plant Viruses245
    [Google Scholar]
  23. HUNST P. L., TOLIN S. A. 1982; Isolation and comparison of two strains of soybean mosaic virus. Phytopathology 72:710–713
    [Google Scholar]
  24. KOENIG R., LESEMANN D. E. 1979; Tymovirus group. CMI/AAB Descriptions of Plant Viruses214
    [Google Scholar]
  25. MAISS E., BREYEL E., BRISSKE A., CASPER R. 1988; Molecular cloning of cDNA complementary to the RNA genome of plum pox virus (PPV). Journal of Phytopathology 122:222–231
    [Google Scholar]
  26. MATTHEWS R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  27. NELSON M. R., WHEELER R. E. 1978; Biological and serological characterization and separation of potyviruses that infect peppers. Phytopathology 68:979–984
    [Google Scholar]
  28. NESTOROWICZ A., KAWAOKA Y., BEAN W. J., WEBSTER R. G. 1987; Molecular analysis of the hemagglutinin genes of Australian H7 N7 influenza viruses: role of passerine birds in maintenance or transmission. Virology 160:411–418
    [Google Scholar]
  29. PURCIFULL D. E., HIEBERT E., MCDONALD J. G. 1973; Immunochemical specificity of cytoplasmic inclusions induced by viruses in the potato Y group. Virology 55:275–279
    [Google Scholar]
  30. PURCIFULL D. E., ZITTER T. A., HIEBERT E. 1975; Morphology, host range and serological relationships of pepper mottle virus. Phytopathology 65:559–562
    [Google Scholar]
  31. RAVELONANDRO M., VARVERI C, DELBOS R., DUNEZ J. 1988; Nucleotide sequence of the capsid protein gene of plum pox potyvirus. Journal of General Virology 69:1509–1516
    [Google Scholar]
  32. RAYMOND F. L., CATON A. J., COX N. J., KENDAL A. P., BROWNLEE G. G. 1986; The antigenicity and evolution of influenza H1 hemagglutinin, from 1950-1957 and 1977-1983: two pathways from one gene. Virology 148:275–287
    [Google Scholar]
  33. SHUKLA D. D., INGLIS A. S., MCKERN N. M., GOUGH K. H. 1986; Coat protein of potyviruses. 2. Amino acid sequence of coat protein of potato virus Y. Virology 152:118–125
    [Google Scholar]
  34. SHUKLA D. D., GOUGH K. H., WARD C. W. 1987; Coat protein of potyviruses. 3. Comparison of amino acid sequences of the coat proteins of four Australian strains of sugarcane mosaic virus. Archives of Virology 96:59–74
    [Google Scholar]
  35. SHUKLA D. D., JILKA J., TOSIC M., FORD R. E. 1988a; A novel approach to serology of potyviruses involving affinity-purified polyclonal antibodies directed towards virus-specific N termini of coat proteins. Journal of General Virology in press
    [Google Scholar]
  36. SHUKLA D. D., MCKERN N. M., GOUGH K. H., TRACY S. L., LETHO S. G. 1988b; Differentiation of potyviruses and their strains by high performance liquid chromatographic peptide profiling of coat proteins. Journal of General Virology 69:493–502
    [Google Scholar]
  37. SHUKLA D. D., MCKERN N. M., WARD C. W. 1988c; Coat protein of potyviruses. 5. Symptomatology, serology and coat protein sequences of three strains of passion-fruit woodiness virus. Archives of Virology in press
    [Google Scholar]
  38. SHUKLA D. D., STRIKE P. M., TRACY S. L., GOUGH K. H., WARD C. W. 1988d; The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. Journal of General Virology 69:1497–1508
    [Google Scholar]
  39. SHUKLA D. D., THOMAS J. E., MCKERN N. M., TRACY S. L., WARD C. W. 1988e; Coat protein of potyviruses. 4. Comparison of biological properties, serological relationships and coat protein amino acid sequences of four strains of potato virus Y. Archives of Virology in press
    [Google Scholar]
  40. SHUKLA D. D., TOSIC M., JILKA J., FORD R. E., TOLER R. W., LANGHAM M. 1989; Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology in press
    [Google Scholar]
  41. THORNBURY D. W., HELLMAN G. M., RHOADS R. E., PIRONE T. P. 1985; Purification and characterization of potyvirus helper component. Virology 144:260–267
    [Google Scholar]
  42. VAN REGENMORTEL M. H. V. 1986 Tobacco mosaic virus: antigenic structure. The Plant Viruses: The Rod Shaped Plant Viruses 279–104 Edited by Van Regenmortel M. H. V., Fraenkel-Conrat H. New York & London: Plenum Press;
    [Google Scholar]
  43. VANCE V. B., BEACHY R. N. 1984; Translation of soybean mosaic virus RNA in vitro: evidence of protein processing. Virology 132:271–281
    [Google Scholar]
  44. WARD C. W. 1981; Structure of the influenza virus haemagglutinin. Current Topics in Microbiology and Immunology 94/95:1–74
    [Google Scholar]
  45. WILSON A. C, CARLSON S. S., WHITE T. J. 1977; Biochemical evolution. Annual Review of Biochemistry 46:573–639
    [Google Scholar]
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