1887

Abstract

Summary

The genome of the autonomous parvovirus minute virus of mice (MVM) is organized in two overlapping transcription units: the genes coding for the two non-structural proteins (NS-1 and NS-2) are transcribed from a promoter (P04) located at map unit 4, whereas the promoter controlling the capsid protein genes (P39) lies at map unit 39. We studied the effect of viral proteins on the activity of the P39 promoter . By site-directed mutagenesis we constructed clones encoding only one of the two NS proteins. The activity of the P39 promoter was measured in HeLa or EL-4 cells transfected with these clones, either by an RNase protection assay or by following the expression of a reporter gene, CAT (which codes for chloramphenicol acetyltransferase), placed under the control of this promoter. We found that the P39 promoter of strain MVMi is activated by a viral gene product, and evidence to suggest that NS-1 is the only viral gene product responsible for this -activation. We also determined that the mechanism of -activation is very rapid, since all species of viral mRNAs appear together in non-synchronized infected EL-4 cells within a 2 h interval.

Keyword(s): MVM , parvovirus and trans-activation
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-10-2563
1988-10-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/10/JV0690102563.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-10-2563&mimeType=html&fmt=ahah

References

  1. ANTONIETTI J.-PH., SAHLI R., BEARD P., HIRT B. 1987; Characterization of the cell type-specific determinant in the genome of minute virus of mice. Journal of Virology 62:552–557
    [Google Scholar]
  2. ASTELL C. R., THOMSON M., MERCHLINSKY M., WARD D. C. 1983; The complete DNA sequence of minute virus of mice, an autonomous parvovirus. Nucleic Acids Research 11:999–1018
    [Google Scholar]
  3. ASTELL C. R., GARDINER E. M., TATTERSALL P. 1986; DNA sequence of the lymphotropic variant of minute virus of mice, MVMi, and comparison with the DNA sequence of the fibrotropic strain. Journal of Virology 57:656–669
    [Google Scholar]
  4. BANERJI J., OLSON L., SCHAFFNER W. 1983; A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. Cell 33:729–740
    [Google Scholar]
  5. BARNES W. M., BEVAN M. 1983; Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition. Nucleic Acids Research 11:349–368
    [Google Scholar]
  6. BERNS K. 1984 The Parvoviruses New York & London: Plenum Press;
    [Google Scholar]
  7. BONNARD G. D., MANDERS E. K., CAMPBELL D. A., HERBERMANN R. B., COLLINS M. J. 1976; Immunosuppressive activity of a subline of the mouse EL-4 lymphoma: evidence for minute virus of mice causing the inhibition. Journal of Experimental Medicine 143:187–205
    [Google Scholar]
  8. BRADY J., BOLEN J. B., RADONOVICH M., SALZMAN N., KHOURY G. 1984; Stimulation of simian virus 40 late gene expression by simian virus tumor antigen. Proceedings of the National Academy of Sciences, U.S.A 81:2040–2044
    [Google Scholar]
  9. CLEMENS K. E., PINTEL D. J. 1987; Minute virus of mice mRNAs predominantly polyadenylate at a single site. Virology 160:511–514
    [Google Scholar]
  10. CLEMENS K. E., PINTEL D. J. 1988; The two transcription units of the autonomous parvovirus minute virus of mice are transcribed in a temporal order. Journal of Virology 62:1448–1451
    [Google Scholar]
  11. COTMORE S. F., TATTERSALL P. 1986; Organization of nonstructural genes of the autonomous parvovirus minute virus of mice. Journal of Virology 58:724–732
    [Google Scholar]
  12. COTMORE S. F., TATTERSALL P. 1988; The NS-1 polypeptide of minute virus of mice is covalently attached to the 5′ termini of duplex replicative-form DNA and progeny single strands. Journal of Virology 62:851–860
    [Google Scholar]
  13. CRAWFORD L. V. 1966; A minute virus of mice. Journal of Virology 29:605–612
    [Google Scholar]
  14. DENTE L., CESARENI G., CORTESE R. 1983; pEMBL: a new family of single stranded plasmids. Nucleic Acids Research 11:1645–1655
    [Google Scholar]
  15. ENGERS H. D., LOUIS J. A., ZUBLER R. H., HIRT B. 1981; Inhibition of T cell-mediated functions by MVMi, a parvovirus closely related to the minute virus of mice. Journal of Immunology 127:2280–2285
    [Google Scholar]
  16. GORMAN C. M., MOFFAT L. F., HOWARD B. H. 1982a; Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  17. GORMAN C. M., MERLINO G. T., WILLINGHAM M. C, PASTAN I., HOWARD B. H. 1982b; The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eucaryotic cells by DNA mediated transfection. Proceedings of the National Academy of Sciences, U.S.A 79:6777–6781
    [Google Scholar]
  18. GRAHAM F. L., VAN DER EB A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–463
    [Google Scholar]
  19. GREEN M. R., MANIATIS T., MELTON D. A. 1983; Human α-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell 32:681–694
    [Google Scholar]
  20. IONGENEEL C. V., SAHLI R., MCMASTER G. K., HIRT B. 1986; A precise map of splice junction in the mRNAs of minute virus of mice, an autonomous parvovirus. Journal of Virology 59:564–573
    [Google Scholar]
  21. KELLER J. M., ALWINE J. C. 1984; Activation of the SV40 late promoter: direct effects of T antigen in the absence of viral DNA replication. Cell 36:381–389
    [Google Scholar]
  22. MCMASTER G. K., BEARD P., ENGERS H. D., HIRT B. 1981; Characterization of an immunosuppressive parvovirus related to the minute virus of mice. Journal of Virology 38:317–326
    [Google Scholar]
  23. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. MELTON D. A., KRIEG P. A., REBAGLIATI M. R., MANIATIS T., ZINN K., GREEN M. R. 1984; Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Research 12:7035–7056
    [Google Scholar]
  25. NEVINS J. R. 1981; Mechanism of activation of early viral transcription by the adenovirus E1A gene product. Cell 26:213–220
    [Google Scholar]
  26. OWEN D., KÜHN L. C. 1987; Noncoding 3′ sequences of the transferrin receptor gene are required for mRNA regulation by iron. EMBO Journal 6:1287–1293
    [Google Scholar]
  27. PINTEL D., DADACHANJI D., ASTELL C. R., WARD D. C. 1983; The genome of minute virus of mice, an autonomous parvovirus, encodes two overlapping transcription units. Nucleic Acids Research 11:1019–1038
    [Google Scholar]
  28. POST L., MACKEM S., ROIZMAN B. 1981; Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters. Cell 24:555–566
    [Google Scholar]
  29. RHODE S. L. III 1985; Transactivation of parvovirus P38 promoter by the 76K noncapsid protein. Journal of Virology 55:886–889
    [Google Scholar]
  30. RHODE S. L. M., RICHARD S. M. 1987; Characterization of the transactivation-responsive element of the parvovirus H-1 P38 promoter. Journal of Virology 61:2807–2815
    [Google Scholar]
  31. SAHLI R., MCMASTER G. K., HIRT B. 1985; DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice. Nucleic Acids Research 13:3617–3633
    [Google Scholar]
  32. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  33. SOMPAYRAC L. M., DANNA K. J. 1981; Efficient infection of monkey cells with DNA of simian virus 40. Proceedings of the National Academy of Sciences, U.S.A 78:7575–7578
    [Google Scholar]
  34. TATTERSALL P., BRATTON J. 1983; Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice. Journal of Virology 46:944–955
    [Google Scholar]
  35. WARD D. C., TATTERSALL P. 1978 Replication of Mammalian Parvoviruses New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  36. ZINN K., DIMALO D., MANIATIS T. 1983; Identification of two distinct regulatory regions adjacent to the human beta interferon gene. Cell 34:865–879
    [Google Scholar]
  37. ZOLLER M. J., SMITH M. 1983; Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods in Enzymology 100:468–500
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-10-2563
Loading
/content/journal/jgv/10.1099/0022-1317-69-10-2563
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error