1887

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Volume 69, Issue 10

Humoral and Cytotoxic T Cell Immune Responses to Internal and External Structural Proteins of Simian Virus 5 Induced by Immunization with Solid Matrix-Antibody-Antigen Complexes Free

Abstract

Summary

Monoclonal antibodies (MAbs) were complexed to solid matrices and used to purify virus proteins from simian virus 5 (SV5)-infected tissue culture cells, ideally in such a way as to bind equimolar amounts of antigen to antibody. The resulting solid matrix-antibody-antigen (SMAA) complexes were then used as immunogens and successfully induced specific humoral and cytotoxic T cell responses. By attaching more than one MAb to the solid matrix and using such complexes to purify the respective proteins multivalent immunization was achieved. Analysis of the cytotoxic T cell response of immunized animals indicated that both surface and internal SV5 structural proteins can act as target antigens. Immunization with SMAA complexes, in the absence of adjuvant, induced higher levels of antibody than the antigen alone precipitated on alum. However, immunization with SMAA complexes resulted in relatively less antibody being produced to the antigenic determinant through which the protein is coupled, via antibody, to the SMAA complex compared with the amount of antibody produced against other antigenic determinants on that protein. The particulate solid matrix used to form the SMAA complexes in most of the experiments was a ‘fixed’ and killed suspension of the Cowan A strain of , although preliminary results indicated that Protein A-Sepharose could also be used as a solid matrix. Prior immunization with alone did not reduce the level of the immune response to the appropriate antigen on subsequent immunization with -antibody-antigen complexes. In fact on immunization of mice with these complexes the level of antibody produced to the matrix itself was less when -antibody-antigen complexes were used as immunogens than when or -antibody complexes were used. Furthermore, rabbits immunized with -mouse MAb-antigen complexes showed a vigorous immune response to the antigen.

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Keyword(s): immune response , paramyxoviruses and simian virus 5
© Society for General Microbiology 1988
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1988-10-01
2024-04-25
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References

  1. ADAMS A. 1985 Synthetic adjuvants. Modern Concepts in Immunology New York: John Wiley - Interscience;
     [Google Scholar]
  2. BANGHAM C. R. M., CANNON M. J., KARZON D. T., ASKONAS B. A. 1985; Cytotoxic T-cell response to respiratory syncytial virus in mice. Journal of Virology 56:55–59
     [Google Scholar]
  3. CHASE M. W. 1967 Preparation of immunogens. Methods in Immunology and Immunochemistry 1 Preparation of Antigens and Antibodies 197–200 Edited by Williams C. A., Chase M. W. London: Academic Press;
     [Google Scholar]
  4. GOSWAMI K. K. A., LANGE L. S., MITCHELL D. N., CAMERON K. R., RUSSELL W. C. 1984; Does simian virus 5 infect humans?. Journal of General Virology 65:1295–1303
     [Google Scholar]
  5. GOSWAMI K. K. A., RANDALL R. E., LANGE L. S., RUSSELL W. C. 1987; Antibodies against the paramyxovirus SV5 in the cerebrospinal fluids of some multiple sclerosis patients. Nature, London 327:244–247
     [Google Scholar]
  6. JAMES K., BELL G. T. 1987; Human monoclonal antibody production. Current status and future prospects. Journal of Immunological Methods 100:5–40
     [Google Scholar]
  7. JOHANSSON M. E., WADELL G., JACOBSSON P., SVENSSON L. 1979; Preparation of specific antisera against adenoviruses by affinity bead immunization (ABI). Journal of Immunological Methods 26:141–149
     [Google Scholar]
  8. KESSLER S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein-A absorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
     [Google Scholar]
  9. KLAUS G. G. B. 1988 Role of complement in the induction of antibody responses. The Complement System Edited by Rother K., Till G. O. Wien & New York: Springer-Heidelberger;
     [Google Scholar]
  10. MOREIN B., LOVGREN K., HOGLUND S., SUNDQUIST B. 1987; The ISCOM: an immunostimulating complex. Immunology Today 8:333–338
     [Google Scholar]
  11. PEMBERTON R. M., CANNON M. J., OPENSHAW P. J. M., BALL L. A., WERTZ G. W., ASKONAS B. A. 1987; Cytotoxic T cell specificity for respiratory syncytial virus proteins: fusion protein is an important target antigen. Journal of General Virology 68:2177–2182
     [Google Scholar]
  12. RANDALL R. E., NEWMAN C., HONESS R. W. 1984; Isolation and characterization of monoclonal antibodies to structural and nonstructural proteins of herpesvirus saimiri proteins. Journal of Virology 52:872–883
     [Google Scholar]
  13. RANDALL R. E., YOUNG D. F., GOSWAMI K. K. A., RUSSELL W. C. 1987; Isolation and characterization of monoclonal antibodies to simian virus 5 and their use in revealing antigenic differences between human, canine and simian isolates. Journal of General Virology 68:2769–2780
     [Google Scholar]
  14. RANDALL R. E., YOUNG D. F., SOUTHERN J. A. 1988; Immunization with solid matrix-antibody-antigen complexes containing surface or internal virus structural proteins protects mice from infection with the paramyxovirus, simian virus 5. Journal of General Virology 69:2517–2526
     [Google Scholar]
  15. RIECHMANN L., CLARK M., WALDMANN H., WINTER G. 1988; Reshaping human antibodies for therapy. Nature, London 332:323–327
     [Google Scholar]
  16. WATSON D. H., WILDY P. 1969; The preparation of ‘monoprecipitin’ antisera to herpes virus specific antigens. Journal of General Virology 4:163–168
     [Google Scholar]
  17. ZWEERINK J. H., ASKONAS B. A., MILLICAN D., COURTNEIDGE S. A., SKEHEL J. J. 1977; Cytotoxic T-cells to type A influenza virus: viral haemagglutinin induces A-strain specificity while infected cells confer cross-reactive cytotoxicity. European Journal of Immunology 7:630–635
     [Google Scholar]
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Humoral and Cytotoxic T Cell Immune Responses to Internal and External Structural Proteins of Simian Virus 5 Induced by Immunization with Solid Matrix-Antibody-Antigen Complexes
J. Gen. Virol. 69, 2505 (1988); https://doi.org/10.1099/0022-1317-69-10-2505
/content/journal/jgv/10.1099/0022-1317-69-10-2505
/content/journal/jgv/10.1099/0022-1317-69-10-2505
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